Springer eBooks, Journal Year: 2020, Volume and Issue: unknown, P. 49 - 68
Published: Jan. 1, 2020
Language: Английский
BioTechniques, Journal Year: 2020, Volume and Issue: 68(5), P. 283 - 290
Published: March 23, 2020
The application of RNAi promotes the development novel approaches toward plant protection in a sustainable way. Genetically modified crops expressing dsRNA have been developed as commercial products with great potential insect pest management. Alternatively, some nontransformative approaches, including foliar spray, irrigation and trunk injection, are favorable actual utilization. In this review, we summarize recent progress successful cases RNAi-based management strategy, explore essential implications possibilities to improve efficiency by delivery through transformative highlight remaining challenges important issues related technology.
Language: Английский
Citations
121
Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12
Published: Oct. 15, 2021
The drastic loss of biodiversity has alarmed the public and raised sociopolitical demand for chemical pesticide-free plant production, which is now treated by governments worldwide as a top priority. Given this global challenge, RNAi-based technologies are rapidly evolving promising substitute to conventional pesticides. Primarily, genetically modified (GM) crops expressing double-stranded (ds)RNA-mediating gene silencing foreign transcripts have been developed. However, since cultivation GM RNAi viewed negatively in numerous countries, GM-free exogenous RNA spray applications attract tremendous scientific political interest. sudden rise pesticide alternatives boosted research on sprayable biopesticides, generating significant technological developments advancing potential field near future. Here we review latest advances that could pave way quick lab-to-field transition sprays, which, safe, selective, broadly applicable, cost-effective represent an innovation sustainable crop production. these advances, further discuss limitations, knowledge gaps research, safety concerns regulatory requirements need be considered addressed before sprays can become reliable realistic agricultural approach.
Language: Английский
Citations
73
Insect Science, Journal Year: 2021, Volume and Issue: 29(3), P. 669 - 682
Published: July 20, 2021
RNA interference (RNAi) has developed rapidly as a potential "green" pest management strategy. At present, most studies have focused on the screening of aphid lethal genes, whereas only few been conducted wing development, which is crucial for migration and plant-virus dissemination. Here, Myzus persicae genes vestigial (vg) Ultrabithorax (Ubx) related to were cloned. These two expressed in various tissues 3rd-instar winged aphids. The mRNA level vg was high nymphs, expression Ubx adults. nanocarrier-mediated delivery system delivered double-stranded RNAs RNAi using topical root applications. levels downregulated by 44.0% 36.5%, respectively, application. simultaneous target caused 63.3% 32.2% aberration rates applications, respectively. current study provided promising method controlling alleviate spread insect transmitted plant diseases.
Language: Английский
Citations
62
Journal of Pest Science, Journal Year: 2022, Volume and Issue: 96(1), P. 181 - 193
Published: Feb. 18, 2022
Language: Английский
Citations
58
Agriculture, Journal Year: 2024, Volume and Issue: 14(2), P. 284 - 284
Published: Feb. 9, 2024
Recent advancements in molecular biology have revolutionized plant disease diagnosis and management. This review focuses on through serological techniques, isothermal amplification methods, CRISPR-based approaches, management strategies using RNA-based methods. Exploring high-throughput sequencing RNA interference (RNAi) technologies like host-induced gene silencing (HIGS) spray-induced (SIGS), this delves into their potential. Despite the precision offered by RNAi pest pathogen management, challenges such as off-target effects efficient dsRNA delivery persist. discusses significance of these preventing aphid-mediated virus transmission, emphasizing crucial role meticulous design for effective viral targeting while minimizing harm to RNA. acknowledged challenges, including issues, underscores transformative potential agriculture. Envisaging reduced pesticide dependency enhanced productivity, stand key players future sustainable
Language: Английский
Citations
12
Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 209, P. 106330 - 106330
Published: Feb. 19, 2025
Language: Английский
Citations
1
Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11
Published: June 4, 2020
RNA interference (RNAi) is a powerful technology that offers new opportunities for pest control through silencing of genes are essential the survival arthropod pests. The approach relies on sequence-specificity applied double-stranded (ds) can be designed to have very narrow spectrum both target gene product (RNA) as well organism, and thus allowing highly targeted control. Successful RNAi has been reported from number species belonging various orders. Pest may achieved by applying dsRNA foliar sprays. One main concerns related use adverse environmental effects particularly valued non-target species. Arthropods form an important part biodiversity in agricultural landscapes contribute ecosystem services. Consequently, risk assessment (ERA) potential impacts plant protection products arthropods legally required prior their placement market. We describe how problem formulation used set context develop plausible pathways application dsRNA-based could harm species, such those contributing biological current knowledge regarding exposure hazard posed spray reviewed suggestions provided select most suitable test conduct laboratory-based toxicity studies provide robust, reliable interpretable results support ERA.
Language: Английский
Citations
62
Journal of Pest Science, Journal Year: 2021, Volume and Issue: 95(1), P. 1 - 15
Published: Nov. 25, 2021
Language: Английский
Citations
48
Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10
Published: June 28, 2022
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas) system and RNA interference (RNAi)-based non-transgenic approaches are powerful technologies capable of revolutionizing plant research breeding. In recent years, the use these modern has been explored in various sectors agriculture, introducing or improving important agronomic traits crops, such as increased yield, nutritional quality, abiotic- and, mostly, biotic-stress resistance. However, limitations each technique, public perception, regulatory aspects hindering its wide adoption for development new crop varieties products. an attempt to reverse mishaps, scientists have researching alternatives increase specificity, uptake, stability CRISPR RNAi components target organism, well reduce chance toxicity nontarget organisms minimize environmental risk, health problems, issues. this review, we discuss several related risk assessment, toxicity, advances CRISPR/Cas topical RNAi-based management The present study also highlights advantages possible drawbacks technology, provides a brief overview how circumvent off-target occurrence, strategies on-target harm/benefits association with nanotechnology, perception available techniques, worldwide frameworks regarding technologies, lastly, presents successful case studies biotechnological solutions derived from both raising potential challenges reach market being social environmentally safe.
Language: Английский
Citations
33
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 271, P. 132455 - 132455
Published: May 23, 2024
Language: Английский
Citations
8