Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106160 - 106160
Published: Oct. 3, 2024
Language: Английский
Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Sitophilus zeamais is a highly destructive pest of stored grains worldwide. To develop novel RNA insecticides, the double-stranded S. cytochrome c oxidase (dsSzCOXs: dsSzCOXI, dsSzCOXII, and dsSzCOXIII) was successfully expressed in pET28a-BL21(DE3)-RNaseIII- system remarkably downregulated expression level SzCOXs through injection or feeding methods, leading to high mortalities both larvae adults. Under their optimal conditions (isopropyl β-d-thiogalactoside, 0.6 mM), yields dsSzCOXI (at 28 °C for 4 h), dsSzCOXII 37 6 dsSzCOXIII h) were 8.49, 5.80, 6.04 μg/mL respectively. The chitosan-based gene delivery composed dsSzCOXs (CS@dsSzCOXs) established insecticidal effect adults via mixing wheat seeds, with mortality reaching 50% on day ten. Consequently, this nanoparticle-mediated expected be an effective approach control.
Language: Английский
Citations
0
Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown
Published: March 13, 2025
ABSTRACT RNA interference (RNAi) is increasingly used for plant protection against pathogens and pests. However, the traditional delivery method causes tissue damage, affected by environmental factors, faces difficulties in penetrating barriers of cell walls limitations species, ultimately leading to low efficiency. With advances nanotechnology, nanomaterials (NMs) have been identified as effective carriers nucleic acid because their ability operate independently external mechanical forces, prevent degradation bioenzymes, exhibit good biocompatibility, offer high loading capacity. This review summarizes application NM‐mediated RNAi pests, focusing on how different NMs break through plants, pathogens, pests according size, morphology, charge characteristics. Furthermore, we discuss advantages improvement strategies carriers, alongside assessing potential management
Language: Английский
Citations
0
Insects, Journal Year: 2025, Volume and Issue: 16(4), P. 348 - 348
Published: March 27, 2025
The fall armyworm, Spodoptera frugiperda, a lepidopteran pest from the family Noctuidae, has become major invasive since 2016. Using RNAi methods to control S. frugiperda is currently under investigation. This study first target V-ATPaseD gene of using RNAi. Injection dsRNA-V-ATPaseD into hemolymph 4th-instar larvae significantly suppressed expression at 24 and 48 h post-injection. Treated showed delayed development reduced pupation after 7 days. Subsequently, silencing was achieved through topical or oral administration chitosan/dsRNA-V-ATPaseD nanoparticles. Larvae fed these nanoparticles exhibited significant reductions in mRNA 72 h, persisting until 96 before normalizing. Additionally, treated displayed disrupted molting impaired pupation. Furthermore, were more susceptible emamectin benzoate–lufenuron LC30 concentrations, resulting 68% mortality—27% higher than pesticide alone—72 post-exposure. Combining with enhanced efficacy, providing new insights reduction sustainable for this species.
Language: Английский
Citations
0
Deleted Journal, Journal Year: 2025, Volume and Issue: 77(3)
Published: April 22, 2025
Language: Английский
Citations
0
Agronomy, Journal Year: 2024, Volume and Issue: 14(8), P. 1807 - 1807
Published: Aug. 16, 2024
The most important requirement in sustainable agriculture is to significantly reduce the application of chemical pesticides, and environmentally friendly effective approaches pest management should be established control population size dominant pests. To promote development promising plant protection measures, substantial efforts have been made regarding identification secondary botanical chemicals, pheromones, RNA as well optimization feeding conditions biocontrol agents treatment methods defensive phytohormones. Advances these areas revealed strategies for management, some which widely implemented agricultural practices. Although effectiveness attempts evidenced by their success combating herbivore insects, significant challenges remain. Thus, this review summarizes potential applications associated with alternative currently management. It that a combination will impede evolution pests, leading maximum efficiency
Language: Английский
Citations
3
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 11, 2024
The
Language: Английский
Citations
2
Insects, Journal Year: 2024, Volume and Issue: 15(7), P. 509 - 509
Published: July 7, 2024
Sugar transporters play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells numerous organisms. In insects, sugar not only a role but may also act as receptors virus entry accumulation plant defense compounds. The brown planthopper, Nilaparvata lugens, inflicts damage on rice plants by feeding their phloem sap, which is rich sugars. present study, we identified 34 N. were classified three subfamilies based phylogenetic analysis. motif numbers varied from seven to eleven, motifs 2, 3, 4 functional domains all NlST proteins. Chromosome 1 was found possess highest number genes, harboring 15. gut, salivary glands, fat body, ovary different tissues enriched with gene expression. expression levels NlST2, 4, 7, 20, 27, 28, 31 higher gut than other tissues. When expressed Saccharomyces cerevisiae hexose transporter deletion mutant (strain EBY.VW4000), ApST4 (previously characterized) NlST4, glucose fructose, resulting rescue yeast mutant. These results provide valuable data further studies lugens lay foundation finding potential targets control lugens.
Language: Английский
Citations
1
Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 205, P. 106160 - 106160
Published: Oct. 3, 2024
Language: Английский
Citations
0