CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications

Agriculture, Journal Year: 2022, Volume and Issue: 12(11), P. 1896 - 1896

Published: Nov. 10, 2022

Insect pests impose a serious threat to agricultural productivity. Initially, for pest management, several breeding approaches were applied which have now been gradually replaced by genome editing (GE) strategies as they are more efficient and less laborious. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat/CRISPR-associated system) was discovered an adaptive immune system of bacteria with the scientific advancements, it has improvised into revolutionary technique. Due its specificity easy handling, CRISPR/Cas9-based wide range organisms various research purposes. For control, diverse utilizing CRISPR/Cas9-like systems, thereby making susceptible insecticides, compromising reproductive fitness pest, hindering metamorphosis there many other benefits. This article reviews efficiency proposes potential ideas integrated management. technology successfully insect species. However, is no review available thoroughly summarizes application technique in control. Further, authors highlighted advancements discussed future possibilities

Language: Английский

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Genome editing in pests: basic science to applications

Journal of Pest Science, Journal Year: 2024, Volume and Issue: 97(3), P. 1135 - 1152

Published: Jan. 24, 2024

Language: Английский

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CRISPR-Cas Genome Editing for Insect Pest Stress Management in Crop Plants

Stresses, Journal Year: 2022, Volume and Issue: 2(4), P. 493 - 514

Published: Dec. 7, 2022

Global crop yield and food security are being threatened by phytophagous insects. Innovative methods required to increase agricultural output while reducing reliance on hazardous synthetic insecticides. Using the revolutionary CRISPR-Cas technology develop insect-resistant plants appears be highly efficient at lowering production costs increasing farm profitability. The genomes of both a model insect, Drosophila melanogaster, major insect genera, viz. Spodoptera, Helicoverpa, Nilaparvata, Locusta, Tribolium, Agrotis, etc., were successfully edited toolkits. This new method, however, has ability alter an insect’s DNA in order either induce gene drive or overcome tolerance certain rapid progress methodologies CRISPR their diverse applications show high promise development plant varieties other strategies for sustainable management pests ensure security. paper reviewed critically discussed use genome-editing long-term pest management. emphasis this review was prospective uses system stress through creation genome-edited potential difficulties using reduce examined discussed.

Language: Английский

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Unveiling the Genetic Symphony: Harnessing CRISPR-Cas Genome Editing for Effective Insect Pest Management

Plants, Journal Year: 2023, Volume and Issue: 12(23), P. 3961 - 3961

Published: Nov. 24, 2023

Phytophagous insects pose a significant threat to global crop yield and food security. The need for increased agricultural output while reducing dependence on harmful synthetic insecticides necessitates the implementation of innovative methods. utilization CRISPR-Cas (Clustered regularly interspaced short palindromic repeats) technology develop insect pest-resistant plants is believed be highly effective approach in production expenses enhancing profitability farms. Insect genome research provides vital insights into gene functions, allowing better knowledge biology, adaptability, development targeted pest management disease prevention measures. editing technique has capability modify DNA insects, either trigger drive or overcome their resistance specific insecticides. advancements CRISPR its various applications have shown potential developing insect-resistant varieties other strategies through sustainable approach. This could consequences ensuring involves using create modified plants. article critically analyzed discussed challenges associated with exploring utilizing pressure

Language: Английский

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Multiple independent origins of the female W chromosome in moths and butterflies

Science Advances, Journal Year: 2024, Volume and Issue: 10(25)

Published: June 19, 2024

Lepidoptera, the most diverse group of insects, exhibit female heterogamy (Z0 or ZW), which is different from other insects (male heterogamy, XY). Previous studies suggest a single origin Z chromosome. However, lepidopteran W chromosome remains poorly understood. Here, we assemble genome females down to level model insect ( Bombyx mori ) and identify approximately 10.1 megabase using newly developed tool. In addition, 3593 genes that were not previously annotated in genomes B. . Comparisons 21 species (including 17 ZW four Z0 systems) three trichopteran system) reveal formation Ditrysia involves multiple mechanisms, including proposed canonical noncanonical models, as well mechanism called single-Z turnover. We conclude there are independent origins (most moths all butterflies) Lepidoptera.

Language: Английский

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CRISPR/Cas9: A New Frontier in Biocontrol Strategies for Insect Pest Management

Published: Jan. 1, 2025

Language: Английский

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Identification and functional analysis of the doublesex gene in the mud crab Scylla paramamosain

Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology, Journal Year: 2022, Volume and Issue: 266, P. 111150 - 111150

Published: Jan. 10, 2022

Language: Английский

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The potential of CRISPR-Cas genome editing technologies to mitigate biotic stress in plants

CABI Agriculture and Bioscience, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Language: Английский

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The Sex Determination Cascade in the Silkworm

Genes, Journal Year: 2021, Volume and Issue: 12(2), P. 315 - 315

Published: Feb. 23, 2021

In insects, sex determination pathways involve three levels of master regulators: primary signals, which determine the sex; executors, control sex-specific differentiation tissues and organs; transducers, link signals to executors. The differ widely among insect species. Diptera alone, several unrelated determiners have been identified. However, doublesex (dsx) gene is highly conserved as executor component across multiple orders. transducer level shows an intermediate conservation. many, but not all examined a key role performed by transformer (tra), controls splicing dsx. Lepidoptera, studies focused on lepidopteran model species Bombyx mori (the silkworm). B. mori, signal cascade starts from Fem, female-specific PIWI-interacting RNA, its targeting Masc, apparently specific Lepidoptera. Tra has found in Instead, PSI protein binds directly dsx pre-mRNA regulates alternative produce male- transcripts. Despite this basic understanding molecular mechanisms underlying determination, links transducers executors remain largely unknown review, we focus latest findings regarding functions working genes involved feminization masculinization Lepidoptera discuss directions for future research silkworm.

Language: Английский

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Masculinizer and Doublesex as Key Factors Regulate Sexual Dimorphism in Ostrinia furnacalis

Cells, Journal Year: 2022, Volume and Issue: 11(14), P. 2161 - 2161

Published: July 11, 2022

Sex determination is an important and traditional biological process. In Lepidoptera, Masculinizer (Masc) doublesex (dsx) are the essential genes for sex play critical roles in sexual differentiation development. The functions of Masc dsx have been characterized several model insect species. However, molecular mechanism Ostrinia furnacalis, agricultural pest, still unknown. Here, we successfully used CRISPR/Cas9 genome editing system to knock out OfMasc Ofdsx. Mutation induced male external genital defects sterility. Disruptions Ofdsx common region caused sex-specific genitals adult addition, found that can regulate pigmentation control wing patterns. These results demonstrate key O. suggest novel genetic approaches management pests, including furnacalis.

Language: Английский

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