Genome-Wide Identification, Evolution, and Female-Biased Expression Analysis of Odorant Receptors in Tuta absoluta (Lepidoptera: Gelechiidae)

Life, Journal Year: 2024, Volume and Issue: 14(7), P. 872 - 872

Published: July 12, 2024

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a highly destructive invasive pest targeting Solanaceae crops. Its olfactory system plays crucial role in host location, mate finding, and other behavioral activities. However, there notable gap the literature regarding characterization of its chemosensory genes. In this study, we conducted genome-wide identification 58 odorant receptors (ORs) T. absoluta. identified ORs exhibit coding sequence (CDS) lengths ranging from 1062 bp to 1419 bp, encoding proteins 354 473 amino acids. Gene structure analysis showed that majority these consist five, seven, eight, or nine exons, collectively representing 67% total identified. Through chromosomal mapping, several tandemly duplicate genes, including TabsOR12a, TabsOR12b, TabsOR12c, TabsOR21a, TabsOR21b, TabsOR34a, TabsOR34b, TabsOR34c, TabsOR62a, TabsOR62b. phylogenetic indicated six TabsORs were clustered within lepidopteran sex pheromone receptor clade, while an expansion clade containing ten resulted tandem duplication events. Additionally, five classified into specific OR Furthermore, through RNA-Seq RT-qPCR analyses, (TabsOR21a, TabsOR26a, TabsOR36) exhibiting female-antennae-biased expression. Our study provides valuable foundation further investigations molecular ecological functions TabsORs, particularly relation oviposition behavior. These findings provide foundational data for future exploration female-biased expression genes absoluta, thereby facilitating development eco-friendly attract-and-kill techniques prevention control

Language: Английский

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Role of Enterococcus mundtii in gut of the tomato leaf miner (Tuta absoluta) to detoxification of Chlorantraniliprole

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 204, P. 106060 - 106060

Published: July 30, 2024

Language: Английский

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Emerging Pests and Disease Vectors

Published: Jan. 1, 2025

Language: Английский

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Protected agriculture matters: Year-round persistence of Tuta absoluta in China where it should not.

Entomologia Generalis, Journal Year: 2023, Volume and Issue: 44(2), P. 279 - 287

Published: May 26, 2023

Tuta absoluta (Lepidoptera: Gelechiidae) originates from the South American tropics but has become a major invasive pest of tomato and other Solanaceae crops worldwide.Agricultural protected facilities (APFs) such as greenhouses plastic tunnels may provide thermal conditions that allow survival T. in temperate zones with cold winters.In this study, CLIMEX model was used to investigate dual effects increasing use APFs climate warming on potential distribution seasonal dynamics China.Our showed northern boundary for year-round population persistence China, ignoring APFs, approximately 30°N, covering about 21% China's area suitable under current climate.The modelled increased 31% shifted 40°N 2080 global warming.When APF refuges are included, 78% 79% warming.This suggests that, future, will increase areas at risk invasion significantly more than because effectively protect harsh addition, vegetable production surrounding open fields be during milder seasons when opened can disperse.Therefore, micro-climate should considered part process, Integrated Pest Management simultaneously implemented inside outside rational management absoluta.

Language: Английский

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Genetic diversity and genetic differentiation pattern of Tuta absoluta across China

Entomologia Generalis, Journal Year: 2023, Volume and Issue: 43(6), P. 1171 - 1181

Published: Nov. 24, 2023

Tuta absoluta (Meyrick) is a cosmopolitan invader and one of the most serious pests tomato.This pest has expanded rapidly in China since it initially invaded Xinjiang northwest 2017 Yunnan south early 2018.More complete knowledge migration patterns genetic structure this important for implementing effective management strategies.To fill gap, COI gene T. was sequenced based on nationwide routine monitoring.This study showed new invasion into Gansu Inner Mongolia, indicating ongoing expansion pest.Most populations absoluta, both worldwide, have high homogeneity.Nevertheless, some degree variability found southern China, especially Yunnan.Two distinct haplogroups were distinguished, clade A predominant at global level.The level sequence divergence indicated possible existence cryptic species.Furthermore, F ST analysis molecular variance (AMOVA) revealed weak but significant differentiation between northwestern group group/subgroups provides compelling evidence different dispersal avenues China.T. likely established bridgehead then spread throughout province neighboring territories China.Knowledge regarding diversity, population pattern valuable improving guidelines pest.Strict plant quarantines, local eradication practical control measures are needed to solve problem caused by absoluta.

Language: Английский

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Insight into the host-specificity of a native and a newly introduced parasitoid of Tuta absoluta and prospect for biological control

Biological Control, Journal Year: 2024, Volume and Issue: 191, P. 105464 - 105464

Published: Feb. 16, 2024

New strategies are urgently needed to control Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), one of the most destructive pests tomato crops. Originating from South America, it is spreading rapidly and has evolved resistance common insecticides making highly challenging control. Regarding biological control, parasitoid Necremnus tutae Ribes & Bernardo (Hymenoptera: Eulophidae) widely present around Mediterranean basin adapted invader playing a substantial role in its In addition, neotropical Dolichogenidea gelechiidivoris Marsh Braconidae) established accidentally following host Spain Algeria classical program using this species ongoing Sub-Saharan Africa. Understanding trophic connections between D. gelechiidivoris, N. tutae, targeted T. potential non-target hosts essential assess their value as augmentative or agents promote establishment conservation biocontrol. We conducted host-specificity tests laboratory four European leafminer evaluate specificity these two parasitoids absoluta. then assessed affinity for by exposing sentinel plants with alternative Phthorimaea operculella (Zeller) Gelechiidae) inside greenhouses measuring each species' field parasitism rate. Our results show that tested laboratory, attacked three, while only P. operculella. greenhouse, did not prefer absoluta, whereas preferred latter. The mean rate over three months monitoring period reached 27 % 35 gelechiidivoris. discuss importance understanding effects natural enemy can have

Language: Английский

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First Report on CRISPR/Cas9-Based Genome Editing in the Destructive Invasive Pest Tuta Absoluta (Meyrick) (Lepidoptera: Gelechiidae)

Frontiers in Genetics, Journal Year: 2022, Volume and Issue: 13

Published: May 19, 2022

The tomato leaf miner Tuta absoluta (Meyrick) is one of the world's most destructive pests tomato, and because its severe economic impacts, as well development pesticide resistance, species has been intensively studied, especially in regard to identification targets for T. control. However, functional genomic studies have constrained by a lack effective genetic tools. Therefore, aim present study was develop CRISPR/Cas9 zygote microinjection protocol generating heritable mutations absoluta, using ommochrome synthesis gene cinnabar an easily evaluated target gene. injection fertilised eggs with Cas9 protein four sgRNAs, which targeted exon 3, resulted mutagenesis rate 31.9% reaching adulthood, either red or mosaic eye colour phenotypes. As such, this first report complete detailed workflow efficient genome editing globally important invasive pest absoluta. application robust genome-editing tool will greatly facilitate discovery suitable RNAi control subsequent novel strategies.

Language: Английский

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Risk assessment, cross-resistance, biochemical mechanism and fitness cost of tetraniliprole resistance in the tomato pinworm Tuta absoluta

Crop Protection, Journal Year: 2024, Volume and Issue: 183, P. 106756 - 106756

Published: May 23, 2024

Language: Английский

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Modelling potential distribution of Tuta absoluta in China under climate change using CLIMEX and MaxEnt

Journal of Applied Entomology, Journal Year: 2023, Volume and Issue: 147(10), P. 895 - 907

Published: Sept. 14, 2023

Abstract The Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) originated in South America and is a major pest of the economically critical solanaceous crops. It devastating to tomatoes, attacking mainly leaves, stems, flowers fruits tomato plants, can cause up 100% damage. T. invaded China 2017 expanded rapidly, severely impacting industry. To illustrate detailed potential distribution China, we used CLIMEX MaxEnt models predict this using historical future climate data. predicts wider area for than MaxEnt, which suggests that most suitable its distribution, except Tarim Basin southern Xinjiang, western Inner Mongolia northwestern Gansu. Both accurately predicted known provinces Yunnan Guangxi, predictions by both suggest total range increase slightly with changes temperature. These will help understand influence change on thus provide theoretical foundation developing early monitoring, quarantine control strategies.

Language: Английский

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Odor learning improves efficacy of egg parasitoids as biocontrol agents against Tuta absoluta

Journal of Pest Science, Journal Year: 2022, Volume and Issue: 96(1), P. 105 - 117

Published: Feb. 28, 2022

Abstract Trichogramma spp. (Hymenoptera: Trichogrammatidae) are generalist egg parasitoids widely used as biological control agents, yet with limited efficacy against Tuta absoluta (Meyrick; Lepidoptera: Gelechiidae), a key pest of tomato. Olfactory cues related to the host-plant complex influence searching behavior in parasitoids. Studies have shown that female can learn odor affecting their preference. We assessed if learning increases response tomato and whether it improves parasitism rate achaeae , evanescens, dendrolimi . The were either naive, reared for one generation on T. eggs, or conditioned associatively an oviposition experience factitious host presence odor. Subsequently, arrestment (searching time) -infested leaves was observed static four-chamber olfactometer. Rearing increased time strongly evanescens slightly but not In contrast, (leaves extract) did improve dendrolimi. Concerning improvement, only showed after associative learning. Hence, capacity should be further explored its

Language: Английский

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