Multi-omics approaches for understanding spinetoram resistance in >i<Spodoptera frugiperda>/i< (Lepidoptera: Noctuidae) DOI Creative Commons
Rubens Hideo Kanno

Published: Aug. 1, 2023

Multi-omics approaches for understanding spinetoram resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae)Insecticide is one of the main challenges pest management programs.The fall armyworm, Noctuidae), a polyphagous with high risk evolution to insecticides.Resistance S. has been reported, however, information about molecular basis and possible fitness costs associated it not elucidated.The use multiomics provided insights into mechanisms adaptation processes.Thus, support insecticide managament programs understand frugiperda, this study aimed to: i) characterize employing bulk segragant analysis combined DNA RNA sequencing, ii) assess by comparing several biological parameters constructing fertility life tables resistant, susceptible, heterozygous strains feeding on plants corn, soybean, cotton plants, iii) quantify proteins spinetoram-resistant susceptible when fed soybean using liquid chromatography mass spectrometry (LC-MS), iv) identify metabolites metabolomics-based gas (GC-MS).The genome mapping led identification deletion three nucleotides subunit α6 nicotinic acetylcholine receptor (nAChR α6), transcriptome showed up-regulation some cytochrome P450, ABC transporters cuticle genes that could be involved resistance.Based history traits, there are larvae feed cottons but corn plants.The proteomics host significant factors shaping protein expression strains, majority differentially expressed between these two being specific each plant.Enrichment differential were related metabolic, cellular, developmental, regulation processes.A total 86 detected GC-MS based metabolomics, which 20 abundant strains.The mainly amino acids, carbohydrates, dicarboxylic acids fatty they enriched pathways cysteine methionine metabolism, linoleic acid metabolism aminoacyl-tRNA biosynthesis.The multi-omics allowed comprehensive integrated underlying plant providing holistic perspective adaptive processes.

Language: Английский

Molecular insights into the functional analysis of P450 CYP321A7 gene in the involvement of detoxification of lambda-cyhalothrin in Spodoptera frugiperda DOI
Wanting Li, Jiayu Lin, Jiajie Liu

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 203, P. 106009 - 106009

Published: July 2, 2024

Language: Английский

Citations

4
The power of Drosophila genetics in studying insect toxicology and chemical ecology DOI Creative Commons
Jia Huang, Youngseok Lee

Crop Health, Journal Year: 2023, Volume and Issue: 1(1)

Published: Nov. 27, 2023

Language: Английский

Citations

9
Plasticity of the Gene Transcriptional Level and Microbiota in the Gut Contributes to the Adaptability of the Fall Armyworm to Rice Plants DOI

Wei‐Kang Han,

Feng‐Xian Tang,

Yangyang Yan

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(47), P. 18546 - 18556

Published: Nov. 14, 2023

Insects coordinate a variety of mechanisms to overcome the feeding challenges, including gene transcriptional plasticity and stable symbioses in gut. Here, Spodoptera frugiperda larvae were reared on corn rice plants for successive generations obtain two specific strains. The strain displayed longer developmental period, lower female fecundity, intrinsic growth rate at G1 G5 but not G10. KEGG analysis G1, G5, G11 gut transcriptome indicated that detoxification enzymes might play vital roles host adaptation. RNAi-mediated knockdown CYP12A2 UGT41B8, which highly expressed strain, significantly reduced larval adaptability rice. Besides, dsCYP12A2-treated an increased sensitivity luteolin, flavonoid phytochemical. function prediction microbiota high enrichment level metabolism would essential

Language: Английский

Citations

7
Role of Gut Bacteria in Enhancing Host Adaptation of Tuta absoluta to Different Host Plants DOI Creative Commons
Luo-Hua Shang, Xiang-Yun Cai,

X. Q. Li

et al.

Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 795 - 795

Published: Oct. 13, 2024

The insect gut bacteria play important roles in development and growth, such as immune defense, nutrient metabolism, regulating adaptations for plants, etc.

Language: Английский

Citations

1
HcCYP6AE178 plays a crucial role in facilitating Hyphantria cunea's adaptation to a diverse range of host plants DOI
Tao Li,

Lisha Yuan,

Dun Jiang

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 206, P. 106194 - 106194

Published: Oct. 30, 2024

Language: Английский

Citations

1
An Evaluation of the Crop Preference and Phenotypic Characteristics of Ceracris kiangsu Tsai (Orthoptera: Arcypteridae) under Different Temperatures DOI Creative Commons
Meizhi Wang, Hongmei Li, Abdul Aziz Bukero

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(11), P. 1377 - 1377

Published: Oct. 27, 2023

The yellow-spined bamboo locust (YSBL), Ceracris kiangsu Tsai, has historically had a significant impact on different varieties in East Asia and Southeast Asia. Since 2014, there have been many outbreaks of YSBL populations Laos, YSBLs subsequently invaded Southwest China 2020 2023. However, was limited information about the damage to staple crops. Life table parameters fitness were assessed using wheat, rice, waxy maize, sweet maize under three temperatures (25 °C, 30 35 °C) laboratory. results indicated that feeding wheat seedlings displayed significantly higher survival rate, shorter developmental time, adult emergence rate compared other host species at °C. durations 1st 3rd instar (1st: 8.21 ± 0.35 d; 3rd: 6.32 0.34 d) rice 7.19 0.23 9.00 0.66 than those 13.62 1.22 13.67 6.33 16.00 1.79 18.00 3.49 body lengths male female (male: 29.52 0.40 mm, female: 34.97 0.45 mm) 28.85 0.68 34.66 longer observed when they fed 25.64 1.60 21.93 6.89 mm). There only adults obtained maize. phenotypic characteristics very close seedlings. A relatively slower decline rates nymphs 25 In short, this study implied prefer rice. This is first report direct caused by laboratory, its could be useful improving our understanding preference providing strategies for management pest field

Language: Английский

Citations

3
Transcriptomic and biochemical insights into fall armyworm (Spodoptera frugiperda) responses on silicon-treated maize DOI Creative Commons
Inzamam Ul Haq, Kexin Zhang, Yuping Gou

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e16859 - e16859

Published: Feb. 23, 2024

Background The fall armyworm, Spodoptera frugiperda , is an agricultural pest of significant economic concern globally, known for its adaptability, pesticide resistance, and damage to key crops such as maize. Conventional chemical pesticides pose challenges, including the development resistance environmental pollution. study aims investigate alternative solution: application soluble silicon (Si) sources enhance plant against armyworm. Methods Silicon dioxide (SiO 2 ) potassium silicate (K SiO 3 were applied maize plants via foliar spray. Transcriptomic biochemical analyses performed gene expression changes in armyworm feeding on Si-treated Results indicated a impact expression, with large number differentially expressed genes (DEGs) identified both K treatments. Furthermore, Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis critical DEGs involved specific pathways, amino acid, carbohydrate, lipid, energy, xenobiotics metabolisms, signal transduction, posttranslational modification, significantly altered at Si sources. Biochemical further revealed that treatments inhibited several enzyme activities (glutamate dehydrogenase, trehalase, glucose-6-phosphate chitinase, juvenile hormone esterase, cyclooxygenase while simultaneously inducing others (total protein, lipopolysaccharide, fatty acid synthase, ATPase, cytochrome P450), thus suggesting toxic effect In conclusion, applications influence potentially offering sustainable management strategy.

Language: Английский

Citations

0
The detoxification role of P450 genes implicated in adaptation of Helicoverpa armigera to walnut phytochemistry DOI
Haiqing Li, Xinzheng Huang, Long Yang

et al.

Published: May 24, 2024

Abstract In northwest China, changes in cultivation patterns and the scarcity of preferred hosts have forced Helicoverpa armigera to feed on marginal host walnut (Juglans regia). However, mechanisms allowing this adaptation remain poorly understood. Here, we investigated behavioral, physiological, molecular underlying local pest fruits. Compared kernel, green husk shell generally contained higher levels phytochemicals had greater impact larval feeding preference, fitness, cytochrome P450 activity. These negative effects were further supported by a larger number upregulated detoxification genes insects fed husks or shells based transcriptome sequencing. Together, these data suggest that (such as LOC110371778) may be crucial H. walnuts. Our findings provide significant insight into cotton bollworm walnut, an alternative lower quality.

Language: Английский

Citations

0
Behavioral, Physiological, and Molecular Mechanisms Underlying the Adaptation of Helicoverpa armigera to the Fruits of a Marginal Host: Walnut (Juglans regia) DOI Creative Commons
Haiqiang Li, Xinzheng Huang, Long Yang

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2761 - 2761

Published: Oct. 1, 2024

In northwest China, changes in cultivation patterns and the scarcity of preferred hosts have forced

Language: Английский

Citations

0
Effects of continuous and transgenerational rearing in peanut leaves on the performance and enzyme activity of Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI

Fengluan Yao,

Yaoyao Wu, Shujing Zhou

et al.

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 19, 2024

Abstract The invasive fall armyworm (FAW), Spodoptera frugiperda, is a polyphagous pest that significantly threatens crops worldwide. FAW may undergo adaptation, enhancing its ability to infect specific plant hosts. However, there limited knowledge on this topic. After 8 generations of constant rearing peanut leaves, the performance and enzyme activities were investigated in study. Compared fed cultivars ‘Fuhua 8’ ‘Quanhonghua 1’ for 2 generations, those grown leaves 5 had shorter pre-adult development times total preoviposition periods. Fecundity also increased significantly, resulting an overall improvement population fitness as measured by demographic parameters. F2 generation corn outperformed F8 leaves. In generation, exhibited 30–55% supernumerary larval molts, which decreased substantially F5 generations. Notably, molts displayed pupation emergence rates comparable normal larvae, regardless cultivar or generation. lipase acetylcholinesterase from showing substantial negative positive correlations with time fecundity, respectively. conclusion, demonstrated inferior compared despite was improved after acclimation. These results suggest will continue be primary target crop China.

Language: Английский

Citations

0