Susceptibility of Lymantria dispar to Beauveria bassiana under short-term Cd stress: humoral immunostimulation cannot offset cellular immunotoxicity DOI

Aoying Zhang,

Yaning Li,

Mingtao Tan

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 480, P. 136037 - 136037

Published: Oct. 5, 2024

Language: Английский

Comprehensive analysis of the overexpressed cytochrome P450-based insecticide resistance mechanism in Spodoptera litura DOI
Wenlin Li, Wen Yang, Shi Yao

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 461, P. 132605 - 132605

Published: Sept. 22, 2023

Language: Английский

Citations

29
Lactic acid bacteria modulate the CncC pathway to enhance resistance to β-cypermethrin in the oriental fruit fly DOI Creative Commons
Tian Zeng,

Qianyan Fu,

Fangyi Luo

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract The gut microbiota of insects has been shown to regulate host detoxification enzymes. However, the potential regulatory mechanisms involved remain unknown. Here, we report that bacteria increase insecticide resistance by activating cap “n” collar isoform-C (CncC) pathway through enzymatically generated reactive oxygen species (ROS) in Bactrocera dorsalis. We demonstrated Enterococcus casseliflavus and Lactococcus lactis, two lactic acid-producing bacteria, B. dorsalis β-cypermethrin regulating cytochrome P450 (P450) enzymes α-glutathione S-transferase (GST) activities. These symbionts also induced expression CncC muscle aponeurosis fibromatosis. BdCncC knockdown led a decrease caused bacteria. Ingestion ROS scavenger vitamin C resistant strain affected BdCncC/BdKeap1/BdMafK, resulting reduced GST activity. Furthermore, feeding with E. or L. lactis showed BdNOX5 increased production, BdCncC/BdMafK genes. Moreover, acid activated ROS-associated regulation Collectively, our findings indicate symbiotic modulate intestinal pathways affecting physiological biochemistry, thus providing new insights into involvement insect microbes development resistance.

Language: Английский

Citations

13
Functional Characterization of CYP6QE1 and CYP6FV21 in Resistance to λ-Cyhalothrin and Imidacloprid in Bradysia odoriphaga DOI
Chunni Zhang, Yao Li, Tian Qiu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(6), P. 2925 - 2934

Published: Jan. 30, 2024

Cytochrome P450 monooxygenases (P450s) belong to a family of metabolic enzymes that are involved in the detoxification insecticides. In this study, our bioassay results showed field-collected population Bradysia odoriphaga displayed moderate resistance λ-cyhalothrin and imidacloprid. Compared susceptible population, CYP6QE1 CYP6FV21 were significantly overexpressed field population. The expression was more abundant third fourth larval stages, most highly expressed midgut Malpighian tubules. Exposure imidacloprid increased levels CYP6FV21. Furthermore, silencing susceptibility B. larvae overexpression enhanced tolerance transgenic Drosophila melanogaster lines addition, molecular docking revealed these two proteins have strong binding affinity toward Taken together, indicate is responsible for odoriphaga.

Language: Английский

Citations

7
Transcriptome reveals the roles and potential mechanisms of lncRNAs in the regulation of albendazole resistance in Haemonchus contortus DOI Creative Commons
Xindi Chen,

Tengyu Wang,

Wenrui Guo

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Feb. 17, 2024

Haemonchus contortus (H. contortus) is the most common parasitic nematode in ruminants and prevalent worldwide. H. resistance to albendazole (ABZ) hinders efficacy of anthelmintic drugs, but little known about molecular mechanisms that regulate this drug resistance. Recent research has demonstrated long noncoding RNAs (lncRNAs) can exert significant influence as pivotal regulators emergence

Language: Английский

Citations

5
Contribution of UDP-glycosyltransferases to clothianidin resistance in Bradysia odoriphaga DOI
Chunni Zhang, Ruifang Liu, Xiang‐Long Chen

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 209, P. 106349 - 106349

Published: Feb. 25, 2025

Language: Английский

Citations

0
Dynamic Monitoring of Chilo suppressalis Resistance to Insecticides and the Potential Influencing Factors DOI Creative Commons

Wujia Mo,

Qiang Li, Zhongxian Lü

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 724 - 724

Published: Feb. 27, 2025

Chilo suppressalis is one of the most important rice pests worldwide, and chlorantraniliprole, abamectin, methoxyfenozide have been widely used to control this pest in China. However, efficiency field has dramatically decreased recent years. Therefore, assessing impacts different factors on C. resistance essential for maintaining effectiveness managing resistant populations. Herein, we investigated insecticide its potential influencing (biotic abiotic factors) populations, using bioassays biochemical molecular diagnostic approaches. The results showed that levels populations evolved moderate-to-high tested insecticides. toxicity correlation analysis indicated there was a significant positive between abamectin methoxyfenozide, whereas GST activity positively correlated with suppressalis. EST P450 activities significantly chlorantraniliprole while increase temperature enhanced enzyme evolution methoxyfenozide. Overall, our study provides systematic understanding dynamic status These findings will help clarify development suppressalis, providing theoretical basis management insect species.

Language: Английский

Citations

0
NADPH-cytochrome P450 reductase mediates resistance to neonicotinoid insecticides in Bradysia odoriphaga DOI

Xinxiang Wang,

Xiang‐Long Chen,

Taoling Zhou

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106406 - 106406

Published: April 1, 2025

Language: Английский

Citations

0
RNAi Biopesticides for Root-Feeding Insects DOI
Ivy W. Chen, Stephen J. Fletcher, Wei‐An Tsai

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
A Comparative Study of Transcriptional Regulation Mechanism of Cytochrome P450 CYP6B7 between Resistant and Susceptible Strains of Helicoverpa armigera DOI
Yun Huang, Junyue Zheng, Peizhuo Wu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(24), P. 9314 - 9323

Published: June 8, 2023

Cytochrome P450 CYP6B7 has previously been proved to be associated with fenvalerate-resistance in Helicoverpa armigera. Here, how is regulated and involved the resistance of H. armigera studied. Seven base differences (M1-M7) were found promoter between a fenvalerate-resistant (HDTJFR) susceptible (HDTJ) strain M1-M7 sites HDTJFR mutated into corresponding HDTJ, pGL3-CYP6B7 reporter genes different mutation constructed. Fenvalerate-induced activities at M3, M4, M7 significantly reduced. Transcription factors Ubx Br, whose binding contain M3 M7, respectively, overexpressed HDTJFR. Knockdown Br results significant expression inhibition other resistance-related genes, increase sensitivity fenvalerate. These indicate that regulate mediate

Language: Английский

Citations

8
Investigating the role of the ROS/CncC signaling pathway in the response to xenobiotics in Spodoptera frugiperda using Sf9 cells DOI

Dries Amezian,

Thierry Fricaux,

Georges de Sousa

et al.

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 195, P. 105563 - 105563

Published: Aug. 1, 2023

Language: Английский

Citations

7