Network Pharmacology and Transcriptomics to Explore the Pharmacological Mechanisms of 20(S)-Protopanaxatriol in the Treatment of Depression DOI Open Access

Xiangjuan Guo,

Lili Su,

Meiling Shi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7574 - 7574

Published: July 10, 2024

Depression is one of the most common psychological disorders nowadays. Studies have shown that 20(S)-protopanaxatriol (PPT) can effectively improve depressive symptoms in mice. However, its mechanism needs to be further explored. In this study, we used an integrated approach combining network pharmacology and transcriptomics explore potential mechanisms PPT for depression. First, targets pathways treatment depression were screened through pharmacology. Secondly, BMKCloud platform was obtain brain tissue transcription data chronic unpredictable mild stress (CUMS) model mice screen PPT-altered differential expression genes (DEGs). Gene ontology (GO) analysis Kyoto Encyclopedia Genes Genomes (KEGG) performed using transcriptomics. Finally, above results verified by molecular docking, Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR). demonstrated improved depression-like behavior histopathological changes CUMS mice, downregulated nitric oxide (NO) interleukin-6 (IL-6) levels, elevated serum levels 5-hydroxytryptamine (5-HT) brain-derived neurotrophic factor (BDNF) after compared group. Eighty-seven 350 DEGs identified Comprehensive showed transthyretin (TTR), klotho (KL), FOS, phosphatidylinositol 3-kinase–protein kinase B (PI3K-AKT) signaling pathway closely associated with therapeutic effects PPT. Molecular docking had a high affinity PI3K, AKT, TTR, KL, FOS targets. protein level could increase phosphorylation PI3K (p-PI3K), AKT (p-AKT), KL inhibit depressed Our suggest may achieve inhibiting enhancing TTR modulating PI3K-AKT pathway.

Language: Английский

Xanthones explore the mechanism of p53/p21 signaling pathway to prevent cardiac aging and epigenetic regulation of Nrf2 gene DOI
Yuxin Wei, Yanyan Wang,

Xuebing Jiang

et al.

Archives of Gerontology and Geriatrics, Journal Year: 2025, Volume and Issue: 131, P. 105759 - 105759

Published: Jan. 13, 2025

Language: Английский

Citations

0
Comparative Study of Ginsenoside Rg2, 20(S)-Protopanaxatriol, and Afg from Ginseng on Aging-Related Kidney Injury in Samp8 Mice DOI
Ivan Stève Nguepi Tsopmejio, Jingtian Zhang, Zi Wang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Ginseng in Delaying Brain Aging: Progress and perspectives DOI
Jingwen Niu, Guoqi Zhu, Junjie Zhang

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156587 - 156587

Published: March 1, 2025

Language: Английский

Citations

0
Comparative Study of Ginsenoside Rg2, 20(S)-protopanaxatriol, and AFG from Ginseng on Aging-related Kidney Injury in SAMP8 Mice DOI
Ivan Stève Nguepi Tsopmejio, Jingtian Zhang, Zi Wang

et al.

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119807 - 119807

Published: April 1, 2025

Language: Английский

Citations

0
Eight new triterpenoid saponins from the leaves of Astragalus membranaceus (Fisch.) Bunge and their neuroprotective effects DOI

Wen-xiang Xu,

Zhen-yun Liu,

Qing‐Zhou Bu

et al.

Fitoterapia, Journal Year: 2025, Volume and Issue: 183, P. 106559 - 106559

Published: April 19, 2025

Language: Английский

Citations

0
AFG, an important maillard reaction product in red ginseng, alleviates D-galactose-induced brain aging in mice via correcting mitochondrial dysfunction induced by ROS accumulation DOI
Junjie Zhang,

Ke‐cheng Chen,

Ji-ying Yin

et al.

European Journal of Pharmacology, Journal Year: 2023, Volume and Issue: 952, P. 175824 - 175824

Published: May 31, 2023

Language: Английский

Citations

10
Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis DOI Creative Commons
Shan Tang,

Yun‐yi Hou,

Rui‐yi Hu

et al.

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 115, P. 106102 - 106102

Published: March 5, 2024

Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's anti-aging properties remains unclear. This study established an in vitro D-galactose-induced N2a cells subacute aging model to investigate activity and potential mechanisms. Results indicated that AFG treatment (8 μM) significantly reduces overexpression of aging-related proteins (p53/p21/p16) induced by D-galactose, highlighting its efficacy. concurrently diminishes ROS accumulation, lowering extracellular lactate dehydrogenase alleviating intracellular oxidative stress. Notably, suppresses PERK/CHOP/caspase 12 pathway, reducing Ca2+ concentration mitigating endoplasmic reticulum (ER) Additionally, decreases Bax caspase 3 expression, elevates Bcl-2 mitochondrial membrane potential, apoptosis. NAC with 4-PBA validated results. Overall, protects ER stress apoptosis, suggesting as key substance for treating neurodegenerative diseases.

Language: Английский

Citations

1
Network Pharmacology and Transcriptomics to Explore the Pharmacological Mechanisms of 20(S)-Protopanaxatriol in the Treatment of Depression DOI Open Access

Xiangjuan Guo,

Lili Su,

Meiling Shi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7574 - 7574

Published: July 10, 2024

Depression is one of the most common psychological disorders nowadays. Studies have shown that 20(S)-protopanaxatriol (PPT) can effectively improve depressive symptoms in mice. However, its mechanism needs to be further explored. In this study, we used an integrated approach combining network pharmacology and transcriptomics explore potential mechanisms PPT for depression. First, targets pathways treatment depression were screened through pharmacology. Secondly, BMKCloud platform was obtain brain tissue transcription data chronic unpredictable mild stress (CUMS) model mice screen PPT-altered differential expression genes (DEGs). Gene ontology (GO) analysis Kyoto Encyclopedia Genes Genomes (KEGG) performed using transcriptomics. Finally, above results verified by molecular docking, Western blotting, quantitative real-time polymerase chain reaction (qRT-PCR). demonstrated improved depression-like behavior histopathological changes CUMS mice, downregulated nitric oxide (NO) interleukin-6 (IL-6) levels, elevated serum levels 5-hydroxytryptamine (5-HT) brain-derived neurotrophic factor (BDNF) after compared group. Eighty-seven 350 DEGs identified Comprehensive showed transthyretin (TTR), klotho (KL), FOS, phosphatidylinositol 3-kinase–protein kinase B (PI3K-AKT) signaling pathway closely associated with therapeutic effects PPT. Molecular docking had a high affinity PI3K, AKT, TTR, KL, FOS targets. protein level could increase phosphorylation PI3K (p-PI3K), AKT (p-AKT), KL inhibit depressed Our suggest may achieve inhibiting enhancing TTR modulating PI3K-AKT pathway.

Language: Английский

Citations

0