Plant Secondary Metabolites as Modulators of Mitochondrial Health: An Overview of Their Anti-Oxidant, Anti-Apoptotic, and Mitophagic Mechanisms

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 380 - 380

Published: Jan. 4, 2025

Plant secondary metabolites (PSMs) are a diverse group of bioactive compounds, including flavonoids, polyphenols, saponins, and terpenoids, which have been recognised for their critical role in modulating cellular functions. This review provides comprehensive analysis the effects PSMs on mitochondrial health, with particular emphasis therapeutic potential. Emerging evidence shows that these improve function by reducing oxidative stress, promoting biogenesis, regulating key processes such as apoptosis mitophagy. Mitochondrial dysfunction, hallmark many pathologies, neurodegenerative disorders, cardiovascular diseases, metabolic syndrome, has shown to benefit from protective PSMs. Recent studies show can dynamics, stabilise membranes, enhance bioenergetics, offering significant promise prevention treatment mitochondrial-related diseases. The molecular mechanisms underlying effects, modulation signalling pathways direct interactions proteins, discussed. integration into strategies is highlighted promising avenue improving efficacy while minimising side commonly associated synthetic drugs. also highlights need future research elucidate specific roles individual synergistic within complex plant matrices, may further optimise utility. Overall, this work valuable insights health potential natural agents targeting dysfunction.

Language: Английский

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Ocular Bioanalysis of Moxifloxacin and Ketorolac Tromethamine in Rabbit Lacrimal Matrix Using Liquid Chromatography–Tandem Mass Spectrometry

Bioanalysis, Journal Year: 2024, Volume and Issue: 16(4), P. 219 - 232

Published: Jan. 10, 2024

Aim: The fixed-dose combination of moxifloxacin (MOXI) and ketorolac tromethamine (KTR) is widely used for the treatment bacterial keratitis. Thus, a new LC–MS/MS method was developed to determine MOXI KTR in lacrimal fluid. Methods: Bioanalysis performed using Shimadzu 8050 electrospray ionization-positive mode validated per US FDA guidelines. Isocratic separation with Waters Symmetry C18 column methanol 0.1% formic acid containing deionized water (85:15, v/v). Results & conclusion: An easy, quick selective established applied assess ocular pharmacokinetic profile commercially available formulation KTR. Based on data, this work describes pharmacokinetics-based dosage regimen calculations their clinical significance.

Language: Английский

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Modulation in NF-κB-p65/NLRP3, TXNIP-mediated signaling using an ethanolic fruit extract of Withania coagulans mitigates silica-induced pulmonary fibrosis in rats

Fitoterapia, Journal Year: 2025, Volume and Issue: 183, P. 106578 - 106578

Published: May 1, 2025

Language: Английский

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Codetermination of antimicrobial agents in rabbit tear fluid using LC–MS/MS assay: Insights into ocular pharmacokinetic study

Journal of Mass Spectrometry, Journal Year: 2024, Volume and Issue: 59(6)

Published: May 10, 2024

Abstract Managing ocular microbial infections typically requires pharmacotherapy using antibiotic eye drops, such as moxifloxacin hydrochloride (MFX), combined with an antifungal agent like amphotericin B (AB). We carried out and validated LC–MS/MS assay to quantify these compounds in rabbit tear fluid order look into the pharmacokinetics of two drugs. employed a protein precipitation technique for extraction drugs under examination. A Waters Symmetry C 18 column was used separate analytes internal standard. The composition mobile phase (A) 0.1% v/v formic acid water (B) methanol. detection MFX AB accomplished through utilization positive ion electrospray ionization multiple reaction monitoring mode. linearity curves both exhibited acceptable trendline across concentration range 2.34–300 ng/mL 7.81–1000 surrogate fluid. lower limit quantitation 2.34 ng/mL, while AB, it 7.81 ng/mL. approach strictly validated, encompassing tests selectivity, (with r 2 > 0.99), precision, accuracy, matrix effects, stability. Consequently, we this method evaluate profiles following single topical doses.

Language: Английский

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Hesperidin, a Potential Antiviral Agent against SARS-CoV-2: The Influence of Citrus Consumption on COVID-19 Incidence and Severity in China

Medicina, Journal Year: 2024, Volume and Issue: 60(6), P. 892 - 892

Published: May 28, 2024

This review examines hesperidin, a citrus bioflavonoid, as potential antiviral agent against SARS-CoV-2. The COVID-19 pandemic has demanded an urgent need to search for effective compounds, including those of natural origin, such hesperidin. provides comprehensive analysis the chemical properties, bioavailability and mechanisms particularly its efficacy A databases, PubMedPico, Scopus Web Science, was conducted using specific keywords criteria in accordance with PRISMA (Re-porting Items Systematic Reviews Meta-Analysis) guidelines between 2020 2024. Of 207 articles, 37 were selected review. key aspect is correlation vitro, silico clinical studies on effects hesperidin epidemiological data consumption China during 2020–2024. importance integrating laboratory findings actual patterns better understand role mitigating highlighted, attempt made analyze examine association juice source incidence severity example. identifies consistencies discrepancies experimental data, highlighting correlate two fields Challenges limitations interpreting results future research perspectives this area are discussed. aim bridge gap evidence contribute understanding their correlation.

Language: Английский

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Eco-friendly extraction of hesperidin from citrus peels: a comparative study of Soxhlet, ultrasound-assisted, and microwave-assisted methods for improved yield and antioxidant properties

International Journal of Environmental Health Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: March 26, 2025

This study compares Soxhlet extraction, ultrasound-assisted extraction (UAE), and microwave-assisted (MAE) for isolating hesperidin from citrus peels. UAE achieved the highest yield (89.7%) demonstrated favorable kinetic parameters (rate constant: 0.96 h-1, half-life: 0.72 h), highlighting its efficiency as an eco-friendly alternative. The extract also exhibited total phenolic flavonoid contents, maximizing bioactive compound recovery. While MAE showed superior antioxidant activity in DPPH (IC₅₀: 31.49 µg/mg DE) ABTS 73.38 assays, HPLC analysis confirmed a purity of 89.7%, with yielding comparable results. These findings underscore UAE's potential enhancing while promoting sustainability through waste valorization. research provides valuable insights into optimizing techniques compounds supporting their industrial applications.

Language: Английский

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