Biomacromolecules,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Sugar
beet
pectin,
an
anionic
polysaccharide,
and
silk
fibroin,
a
high
molecular
weight
protein,
undergo
gelation
through
ionic
interactions
conformational
changes,
leading
to
hydrogel
formation.
Although
many
studies
have
focused
on
bulk
gel
systems
involving
polysaccharides
proteins,
more
research
is
needed
investigate
their
properties
at
the
microscale
level.
In
this
context,
we
developed
microgel
system
based
pectin/fibroin
combination
investigated
its
properties.
We
two
mechanisms:
physical
cross-linking
enzymatic
covalent
cross-linking.
The
microgels
were
fabricated
using
droplet-based
microfluidics,
secondary
structure,
mechanical
properties,
degradation
profiles
investigated.
Our
experimental
results
show
that
exhibit
ordered
β-sheet
Young's
modulus
in
range
of
10
20
kPa,
can
be
promoted
protease
enzymes.
Finally,
biocompatibility
assessed
Alamar
Blue
cell
viability
assay
with
human
pulmonary
fibroblasts
(HPFs).
This
presents
highly
functional
hybrid
biomaterial
produced
from
waste
products
structural
demonstrating
compatibility
potential
tissue
engineering
applications.
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: Jan. 19, 2023
Abstract
Living
materials
bring
together
material
science
and
biology
to
allow
the
engineering
augmenting
of
living
systems
with
novel
functionalities.
Bioprinting
promises
accurate
control
over
formation
such
complex
through
programmable
deposition
cells
in
soft
materials,
but
current
approaches
had
limited
success
fine-tuning
cell
microenvironments
while
generating
robust
macroscopic
morphologies.
Here,
we
address
this
challenge
use
core-shell
microgel
ink
decouple
from
structural
shell
for
further
processing.
Cells
are
microfluidically
immobilized
viscous
core
that
can
promote
both
microbial
populations
mammalian
cellular
spheroids,
followed
by
interparticle
annealing
give
covalently
stabilized
functional
scaffolds
controlled
microporosity.
The
results
show
strategy
mitigates
leakage
affording
a
favorable
environment
culture.
Furthermore,
demonstrate
different
consortia
be
printed
into
range
applications.
By
compartmentalizing
separate
microgels,
collective
bioprocessing
capability
scaffold
is
significantly
enhanced,
shedding
light
on
strategies
augment
capabilities.
Small,
Journal Year:
2022,
Volume and Issue:
18(51)
Published: Oct. 19, 2022
Abstract
Hyaluronic
acid
(HA)
is
a
naturally
occurring
polysaccharide
which
has
been
extensively
exploited
in
biomedical
fields
owing
to
its
outstanding
biocompatibility.
Self‐assembly
of
HA
and
polycations
through
electrostatic
interactions
can
generate
colloidal
polyelectrolyte
complexes
(PECs),
offer
wide
range
applications
while
being
relatively
simple
prepare
with
rapid
“green”
processes.
The
advantages
HA‐based
PECs
stem
from
the
combined
benefits
nanomedicine,
green
chemistry,
inherent
properties
HA,
namely
high
biocompatibility,
biodegradability,
biological
targeting
capability.
Accordingly,
have
received
increasing
attention
recent
years
as
high‐performance
materials
for
applications.
Considering
their
potential,
this
review
aimed
provide
comprehensive
understanding
complex
polycations,
most
fundamental
aspects
preparation
process
various
applications,
notably
nanocarriers
delivering
small
molecule
drugs,
nucleic
acids,
peptides,
proteins,
bioimaging
agents
or
construction
multifunctional
platforms.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
35(16)
Published: Feb. 1, 2023
Adequate
vascularization
is
required
for
the
successful
translation
of
many
in
vitro
engineered
tissues.
This
study
presents
a
novel
collagen
derivative
that
harbors
multiple
recognition
peptides
orthogonal
enzymatic
crosslinking
based
on
sortase
A
(SrtA)
and
Factor
XIII
(FXIII).
SrtA-mediated
enables
rapid
co-engineering
human
blood
lymphatic
microcapillaries
mesoscale
capillaries
bulk
hydrogels.
Whereas
tuning
gel
stiffness
determines
extent
neovascularization,
relative
number
recapitulates
ratio
endothelial
cells
originally
seeded
into
hydrogel.
Bioengineered
readily
form
luminal
structures
exhibit
typical
maturation
markers
both
vivo.
The
secondary
enzyme
used
situ
tethering
VEGF
mimetic
QK
peptide
to
collagen.
approach
supports
formation
absence
exogenous
VEGF.
Orthogonal
further
bioengineer
hydrogels
with
spatially
defined
polymer
compositions
pro-
anti-angiogenic
properties.
Finally,
macroporous
scaffolds
microgels
enable
independent
from
supporting
fibroblasts.
Overall,
this
work
demonstrates
first
time
mature
micro-
meso-sized
using
highly
versatile
derivative.
Journal of Colloid and Interface Science,
Journal Year:
2023,
Volume and Issue:
638, P. 363 - 374
Published: Feb. 1, 2023
Organic
solvents
are
often
used
for
cleaning
highly
water-sensitive
artifacts
in
modern/contemporary
art.
Due
to
the
toxicity
of
most
solvents,
confining
systems
must
be
formulated
use
these
fluids
a
safe
and
controlled
way.
We
propose
here
castor
oil
(CO)
organogels,
obtained
thorough
cost-effective
sustainable
polyurethane
crosslinking.
This
methodology
is
complementary
previously
demonstrated
hydrogels,
when
conservators
opt
organic
over
aqueous
formulations.The
gels
were
characterized
via
Small-angle
Neutron
Scattering
rheology
before
after
swelling
two
commonly
adopted
paintings.
The
removal
photo-aged
acrylic-ketonic
varnish
was
evaluated
under
visible
ultraviolet
light,
with
FTIR
2D
imaging.The
new
dry
that
can
easily
stored
loaded
use.
Their
nanoscale
organization,
viscoelasticity
action
changing
amount
crosslinking,
polymeric
backbone,
solvents.
confined
nanosized
mesh
gels,
which
retentive,
granting
release
delicate
paint
layers,
transparent,
allowing
monitoring
process.
These
features,
along
their
synthesis,
candidate
CO
organogels
as
feasible
solutions
cultural
heritage
preservation,
expanding
palette
advanced
tools
traditional
thickeners.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(28), P. 19555 - 19565
Published: July 4, 2024
Gelation
of
protein
condensates
formed
by
liquid–liquid
phase
separation
occurs
in
a
wide
range
biological
contexts,
from
the
assembly
biomaterials
to
formation
fibrillar
aggregates,
and
is
therefore
interest
for
biomedical
applications.
Soluble-to-gel
(sol–gel)
transitions
are
controlled
through
macroscopic
processes
such
as
changes
temperature
or
buffer
composition,
resulting
bulk
conversion
liquid
droplets
into
microgels
within
minutes
hours.
Using
microscopy
mass
spectrometry,
we
show
that
an
engineered
mini-spidroin
(NT2repCTYF)
undergo
spontaneous
sol–gel
transition
loss
exchange
proteins
between
soluble
condensed
phase.
This
feature
enables
us
specifically
trap
silk-domain-tagged
target
spidroin
microgels.
Surprisingly,
laser
pulses
trigger
near-instant
gelation.
By
loading
with
fluorescent
dyes
drugs,
can
control
wavelength
at
which
gelation
triggered.
Fluorescence
reveals
laser-induced
significantly
further
increases
partitioning
molecules
condensates.
In
summary,
our
findings
demonstrate
direct
individual
condensates,
opening
new
avenues
functional
structural
characterization.
Science Advances,
Journal Year:
2024,
Volume and Issue:
10(9)
Published: Feb. 28, 2024
Single-cell
analyses
of
viral
infections
reveal
heterogeneity
that
is
not
detected
by
traditional
population-level
studies.
This
study
applies
drop-based
microfluidics
to
investigate
the
dynamics
herpes
simplex
virus
type
1
(HSV-1)
infection
neurons
at
single-cell
level.
We
used
micrometer-scale
Matrigel
beads,
termed
microgels,
culture
individual
murine
superior
cervical
ganglia
(SCG)
or
epithelial
cells.
Microgel-cultured
cells
are
encapsulated
in
media-in-oil
droplets
with
a
dual-fluorescent
reporter
HSV-1,
enabling
real-time
observation
gene
expression
and
replication.
Infection
within
drops
revealed
kinetics
initial
replication
were
dependent
on
inoculating
dose.
Notably,
increasing
doses
led
earlier
onset
more
frequent
productive
These
observations
provide
crucial
insights
into
complexity
HSV-1
emphasize
importance
studying
outcomes
infection.
techniques
for
cell
foundation
future
virology
neurobiology
investigations.
