Albumin-seeking near-infrared-II probe evaluating blood–brain barrier disruption in stroke

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 29, 2024

Blood-brain barrier (BBB) disruption after stroke is closely associated with brain tissue edema and neuronal injury, which requires accurate assessment. However, there a lack of appropriate BBB imaging modality in vivo. As albumin the blood could cross damaged into stroke, it serves as biomarker for disruption. Therefore, we aimed to develop an albumin-seeking near-infrared (NIR) probe assess stroke. We proposed chemoselective strategy seeking NIR dyes identified optimal evaluate The combined fluorescent dye inherent albumin-targeting moieties exhibited high affinity selectivity binding albumin. Using mouse model, displayed high-resolution visualization location extent vivo correlated well leakage measured by Evans blue ex A dual-channel NIR-II was successfully used simultaneously cerebral perfusion Furthermore, applied this method dynamically process reperfusion thrombolytic therapy model real time, showed excellent application value. developed that accurately evaluated safe, non-invasive real-time manner various models, has great potential guiding treatment manner.

Language: Английский

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Biomimetic Nanocomposites for Glioma Imaging and Therapy

Chemistry - A European Journal, Journal Year: 2024, Volume and Issue: 30(31)

Published: March 28, 2024

Glioma, the most common primary brain tumor, is highly invasive and grows rapidly. As such, survival of glioma patients relatively short, highlighting vital importance timely diagnosis treatment glioma. However, blood barrier (BBB) non-targeting delivery systems contrast agents drugs greatly hinder effective imaging therapy. Fortunately, in recent years, investigators have constructed various biomimetic platforms utilizing exceptional advantages nanocomposites, such as immune evasion, homologous targeting ability, BBB penetrating to achieve efficient precise substances sites for improved treatment. In this concept, we present application these nanocomposites fluorescence (FI), magnetic resonance (MRI), multi-modal imaging, well chemotherapy, phototherapy, combined therapy Lastly, provide our perspective on research field.

Language: Английский

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Enhanced brightness of ultra-small gold nanoparticles in the second biological window through thiol ligand shell control

Journal of Materials Chemistry C, Journal Year: 2023, Volume and Issue: 11(42), P. 14714 - 14724

Published: Jan. 1, 2023

Near-infrared absorption and photoluminescence in the second biological window of ultra-small gold nanoparticles could be tuned by controlling physicochemical properties ligand shell.

Language: Английский

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Bone Marrow Stromal Cells Sorted by Semiconducting Polymer Nanodots for Bone Repair

ACS Biomaterials Science & Engineering, Journal Year: 2023, Volume and Issue: 9(10), P. 5772 - 5781

Published: Sept. 21, 2023

The use of bone marrow stromal cells (BMSCs) for defect repair has shown great promise due to their differentiation potential. However, isolating the BMSCs from various cell types within remains challenging. To tackle this issue, we utilized semiconducting polymer dots (Pdots) as markers select a specific time frame. therapeutic efficacy obtained Pdot-labeled was assessed in model. Initially, evaluated binding capacity Pdots with four different present including BMSCs, osteoblasts, macrophages, and vascular endothelial cells, vitro. Notably, showed most rapid uptake Pdots, being labeled only one h coculture, while other took become labeled. Moreover, by colocalizing Prrx1, Sca-1, OSX, F480, CD105 monocortical tibial (MTD) mice vivo, determined proportions among all 1 8 after injection. It found that have highest proportion (92%) extracted Pdots-labeled (1 h) Results new accrual significantly increased treatment compared cell-treated group. Our study revealed screened could improve repair, suggesting promising application healing.

Language: Английский

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Visualizing Singularity Phenomenon

Biophysics and Physicobiology, Journal Year: 2024, Volume and Issue: 21(Supplemental), P. n/a - n/a

Published: Jan. 1, 2024

In a multicellular system, cells interact and communicate with the surrounding cells.A small number of that considerably impact emerge, either by chance or necessity.When cell-cell interaction centered in these minority triggers an avalanche interactions, system irreversibly undergoes phase transition collective state cells.In case is essential fatal for life phenomena, such as development, metamorphosis, lesions, cell defined "singularity cell," phenomenon."This definition nominal.Systems constantly undergo transitions physical regardless whether observer looking.Based on this definition, humans can explore mechanisms only exist rather than complex combinations principles.In conventional biology, which based statistical quantification using average values, emergence singularity recognized outlier has not been experimentally predicted because its extreme rarity.In recent years, owing to development single-cell omics technologies, experimental data sciences have become more voluminous, enabling detection rare-emerged cells.However, they could prove causal relationship between lack temporal information data.It necessary measure dynamic parameters quantitatively represent screening express detail discover cells.The basic procedure identifying follows: (1) macroscopically detect entire system; (2) microscopically retrogradely search all candidates starting (or) interaction; (3) theoretically mathematically screen candidate causally related transition.Therefore, first principle visualization biology "see forest trees."This means behaviors interactions comprise everywhere, simultaneous observation behavior system.This poses dilemmas measurement systems, example, wide field view high resolution are incompatible optical microscope.Innovations elemental technology will gradually resolve dilemmas; abovementioned incompatibility be overcome specially upsizing apertures objective lens detector [1,2].On other hand, era specification improvements but also simplifying lowering prices needed, various trials biological events what establish new discipline.Research management team overcame dilemma combination simple machinery.They adopted machine vision camera telecentric macro instead at expense three-

Language: Английский

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