N-Deficient B-Doped g-C₃N₄/CdS Heterojunction-Based PEC-FL Biosensor Assisted by CRISPR-Cas12a System for Ultrasensitive Determination of microRNA

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Near-infrared light (NIR)-driven photoelectrochemical (PEC) processes are mainly faced with the limitation of weak photocurrents. Here, N-deficient B-doped g-C3N4/CdS (NB-g-C3N4/CdS) is proposed to construct a NIR-driven PEC biosensor assisted by CRISPR-Cas12a system for determination microRNA-21 (miRNA-21). To promote optical absorption as well separation photogenerated electrons and holes g-C3N4, NB-g-C3N4/CdS constructed via engineering electronic band structure in terms N defect, B doping, heterojunction, achieving high performance. obtain luminescence efficiency exciting under NIR, core–shell NaYF4:Yb3+, Tm3+@NaYF4 upconversion nanoparticles (UCNPs) repaired defects prepared. Furthermore, rolling circle amplification (RCA)-assisted integrated fragment DNA on UCNPs, sensitive detection miRNA-21. On one hand, uncleavaged signal probes UCNPs combined through π–π stacking interaction, generating photocurrents irradiation NIR. other cleavaged which cannot link exhibited fluorescence (FL) signals. The PEC-FL dual-mode provides mutual authentication testing results demonstrates ultrasensitivity (the limit 1.1 fM mode 7.0 FL mode) excellent specificity, promising clinical analysis miRNA.

Language: Английский

---

CRISPR/Cas System: The Accelerator for the Development of Non-nucleic Acid Target Detection in Food Safety

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13577 - 13594

Published: Sept. 1, 2023

Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.

Language: Английский

---

CRISPR/Cas-based nanobiosensors: A reinforced approach for specific and sensitive recognition of mycotoxins

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103110 - 103110

Published: Sept. 9, 2023

Language: Английский

---

Nucleic Acid Enzyme‐Activated CRISPR‐Cas12a With Circular CRISPR RNA for Biosensing

Small, Journal Year: 2023, Volume and Issue: 19(41)

Published: June 9, 2023

clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems are increasingly used in biosensor development. However, directly translating recognition events for non-nucleic acid targets by CRISPR into effective measurable signals represents an important ongoing challenge. Herein, it is hypothesized and confirmed that RNAs (crRNAs) a circular topology efficiently render Cas12a incapable of both site-specific double-stranded DNA cutting nonspecific single-stranded trans cleavage. Importantly, shown nucleic enzymes (NAzymes) with RNA-cleaving activity can linearize the crRNAs, activating CRISPR-Cas12a functions. Using ligand-responsive ribozymes DNAzymes as molecular elements, demonstrated target-triggered linearization crRNAs offers great versatility biosensing. This strategy termed "NAzyme-Activated Circular RNA (NA3C)." Use NA3C clinical evaluation urinary tract infections using Escherichia coli-responsive DNAzyme to test 40 patient urine samples, providing diagnostic sensitivity 100% specificity 90%, further demonstrated.

Language: Английский

---

Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(32), P. 12061 - 12082

Published: Sept. 10, 2023

Food safety problems have become one of the most important public health issues worldwide. Therefore, development rapid, effective and robust detection is great importance. Amongst a range methods, nucleic acid isothermal amplification (NAIA) plays role in food detection. However, widespread application remains limited due to few shortcomings. CRISPR/Cas system has emerged as powerful tool detection, which could be readily integrated with NAIA improve sensitivity, specificity, adaptability versatility dependability. currently there was lack comprehensive summary regarding integration field In this review, recent advances based on CRISPR/Cas-integrated were comprehensively reviewed. To begin with, summarized. Then, types working principles introduced. The applications for mainly introduced objectively discussed. Lastly, current challenges future opportunities proposed. summary, technology expected an approach leading safer more reliable industry.

Language: Английский

---

Self-assembled bifunctional nanoflower-enabled CRISPR/Cas biosensing platform for dual-readout detection of Salmonella enterica

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 471, P. 134323 - 134323

Published: April 16, 2024

Language: Английский

---

Construction of two-dimensional fluorescent covalent organic framework nanospheres for the detection and removal of tetracycline

Separation and Purification Technology, Journal Year: 2023, Volume and Issue: 330, P. 125294 - 125294

Published: Oct. 7, 2023

Language: Английский

---

Emerging programmable nuclease-based detection for food safety

Trends in biotechnology, Journal Year: 2023, Volume and Issue: 42(2), P. 151 - 155

Published: Oct. 7, 2023

Language: Английский

---

Engineering Assembly of Plasmonic Virus‐Like Gold SERS Nanoprobe Guided by Intelligent Dual‐Machine Nanodevice for High‐Performance Analysis of Tetracycline

Small, Journal Year: 2024, Volume and Issue: 20(27)

Published: Jan. 28, 2024

Abstract Accurate detection of trace tetracyclines (TCs) in complex matrices is great significance for food and environmental safety monitoring. However, traditional recognition amplification tools exhibit poor specificity sensitivity. Herein, a novel dual‐machine linkage nanodevice (DMLD) proposed the first time to achieve high‐performance analysis TC, with padlock aptamer component as initiation command center, nucleic acid‐encoded multispike virus‐like Au nanoparticles (nMVANs) signal indicator, cascade walkers circuit processor. The existence spike vertices interspike nanogaps MVANs enables intense electromagnetic near‐field focusing, allowing distinct surface‐enhanced Raman scattering (SERS) activity. Moreover, through sequential activation between multistage walker catalytic circuits, DLMD system converts limited TC into massive engineering assemblies SERS probes guided by DNA amplicons, resulting synergistic enhancement bulk plasmonic hotspot entities. continuously guaranteed target progressively promoted ensure highly specific limit low 7.94 × 10 −16 g mL −1 . Furthermore, reliable recoveries real samples confirm practicability sensing platform, highlighting enormous potential intelligent nanomachines analyzing hazards environment food.

Language: Английский

---

Amplified Assembly of G-Quadruplex-Decorated DNA Network Nanostructure toward AIE Signaling-Based Sensitive Biosensing

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 1749 - 1755

Published: April 8, 2024

Aggregation-induced emission (AIE) has offered a promising approach for developing low-background fluorescent methods; however, its applications often suffer from complex probe synthesis and poor biocompatibility. Herein, novel AIE biosensing method kanamycin antibiotic assays was developed by utilizing DNA network nanostructure assembled an aptamer recognition reaction to capture large number of tetraphenylethylene fluorogen-labeled signal (DTPE) probes. Due the excellent hydrophilicity oligonucleotides, DTPE exhibited water solubility without obvious background emission. Based on ingenious nucleotide design, abundance G-quadruplex blocks neighboring captured were formed nanostructure. Because greatly restricted free motion this unique nanostructure, strong fluorescence response produced construct transduction strategy. Together with target recycling rolling circle amplification-based cascade nucleic acid amplification, wide linear range 75 fg mL–1 1 ng detection limit down 24 mL–1. The analytical performance effective manipulation improvement over previous approaches determine potential various applications.

Language: Английский

---