CRISPR/Cas12a-Powered Gold/Nickel Foam Surface-Enhanced Raman Spectroscopy Biosensor for Nucleic Acid specific Detection in foods DOI
Yan Liu,

Shirui Gou,

Long Qiu

et al.

The Analyst, Journal Year: 2024, Volume and Issue: 149(17), P. 4343 - 4350

Published: Jan. 1, 2024

Food is a necessary source of energy, but it also serves as pathway for transmitting infectious pathogens, making food safety matter great concern. Rapid, accurate, and specific detection methods foodborne viruses are crucial. Surface-Enhanced Raman Scattering (SERS), due to its superior sensitivity characteristic fingerprint spectra, holds enormous potential. However, the limitations SERS, requires conditions achieve specificity. In order enhance specificity accuracy nucleic acid based on we have developed CRISPR-Cas12a-mediated SERS technique identify target DNA, harnessing targeting recognition capability CRISPR-Cas12a ultra-sensitive tags successfully addressing SERS' lack capability. This system includes gold/nickel foam substrate (Au-NFs) reporter (ssDNA-ROX). The phenomenon colloidal gold/silver nano-aggregation magnesium ions, which commonly encountered in CRISPR-SERS, was simultaneously solved using AuNFs. qualitative quantitative analysis DNA drinking water performed by monitoring intensity change ROX molecules. results showed that sensor detected within 30 min limit (LOD) 8.23 fM. expected become one alternative rapid high

Language: Английский

CRISPR/Cas-based nucleic acid detection strategies: Trends and challenges DOI Creative Commons
Jian Zhou, Zhuo Li, Joshua Seun Olajide

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e26179 - e26179

Published: Feb. 1, 2024

CRISPR/Cas systems have become integral parts of nucleic acid detection apparatus and biosensors. Various such as CRISPR/Cas9, CRISPR/Cas12, CRISPR/Cas13, CRISPR/Cas14 CRISPR/Cas3 utilize different mechanisms to detect or differentiate biological activities nucleotide sequences. Usually, CRISPR/Cas-based are combined with polymerase chain reaction, loop-mediated isothermal amplification, recombinase amplification transcriptional technologies for effective diagnostics. Premised on these, many biosensors been developed acids viral bacterial pathogens in clinical samples, well other applications life sciences including biosecurity, food safety environmental assessment. Additionally, showed better specificity compared molecular diagnostic methods. In this review, we give an overview various methods highlight some advances their development components. We also discourse operational challenges advantages disadvantages systems. Finally, important considerations offered the improvement testing.

Language: Английский

Citations

27
CRISPR/Cas-Powered Amplification-Free Detection of Nucleic Acids: Current State of the Art, Challenges, and Futuristic Perspectives DOI
Yaru Li, Yajie Liu, Xiaoqin Tang

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(12), P. 4420 - 4441

Published: Nov. 18, 2023

CRISPR/Cas system is becoming an increasingly influential technology that has been repositioned in nucleic acid detection. A preamplification step usually required to improve the sensitivity of CRISPR/Cas-based The striking biological features CRISPR/Cas, including programmability, high and sequence specificity, single-base resolution. More strikingly, target-activated trans-cleavage could act as a biocatalytic signal transductor amplifier, thereby empowering it potentially perform detection without step. reports such work are on rise, which not only scientifically significant but also promising for futuristic end-user applications. This review started with introduction methods acids diagnostics (CRISPR-Dx). Next, we objectively discussed pros cons steps CRISPR-Dx. We then illustrated highlighted recently developed strategies CRISPR/Cas-powered amplification-free can be realized through uses ultralocalized reactors, cascade reactions, ultrasensitive systems, or others. Lastly, challenges perspectives were proposed. It expected this makes researchers better understand current emerging field, provides insight designing novel CRISPR-Dx win practicable use near future.

Language: Английский

Citations

38
Argonaute-DNAzyme tandem biosensing for highly sensitive and simultaneous dual-gene detection of methicillin-resistant Staphylococcus aureus DOI
Yaru Li, Xiaoqin Tang,

Nan Wang

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 244, P. 115758 - 115758

Published: Oct. 26, 2023

Language: Английский

Citations

30
A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance DOI
San Hae Im, Akhmad Irhas Robby,

Heewon Choi

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(5), P. 5637 - 5647

Published: Jan. 26, 2024

Rapid and accurate diagnosis is crucial for managing the global health threat posed by multidrug-resistant bacterial infections; however, current methods have limitations in either being time-consuming, labor-intensive, or requiring instruments with high costs. Addressing these challenges, we introduce a wireless electrochemical sensor integrating CRISPR/Cas system electroconductive polymer dot (PD) nanoparticles to rapidly detect pathogens from human sputum. To enhance properties, synthesized copper-ion-immobilized PD (PD-Cu), followed conjugation of deactivated Cas9 protein (dCas9) onto PD-Cu-coated Si electrodes generate dCas9-PD-Cu sensor. The integrated isothermal amplification can specifically target nucleic acids bacteria, such as antibiotic resistance genes kpc-2 mecA. exhibits sensitivity, allowing detection ∼54 femtograms acids, based on measuring changes resistivity through capture dCas9. Furthermore, sensing platform was established using Bluetooth module microcontroller unit smartphone. We demonstrate feasibility diagnosing pneumonia patients' sputum samples, achieving 92% accuracy. study presents versatile biosensor that overcome conventional diagnostics clinic.

Language: Английский

Citations

9
CRISPR-based nucleic acid assays for food authentication DOI
Ruijie Deng, Lin Xu, Yong Zhang

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 145, P. 104351 - 104351

Published: Jan. 28, 2024

Language: Английский

Citations

9
An Argonaute-mediated bio-barcode bioassay for one-tube and on-site detection of Staphylococcus aureus DOI
Yaru Li, Yajie Liu, Qiang Zhang

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 410, P. 135713 - 135713

Published: March 27, 2024

Language: Английский

Citations

9
Layer-by-layer (LBL) self-assembly efficient immobilization of glucose oxidase onto PDMS microfluidic chip towards glucose biosensing DOI

Kemeng Zhou,

Yaoyao Yu,

Zhihua Wang

et al.

Journal of Polymer Research, Journal Year: 2025, Volume and Issue: 32(1)

Published: Jan. 1, 2025

Language: Английский

Citations

1
Multiple gRNAs-assisted CRISPR/Cas12a-based portable aptasensor enabling glucometer readout for amplification-free and quantitative detection of malathion DOI

Yi Tian,

Jiaxuan Chen,

Feng‐Zheng Chen

et al.

Analytica Chimica Acta, Journal Year: 2025, Volume and Issue: 1341, P. 343662 - 343662

Published: Jan. 13, 2025

Language: Английский

Citations

1
Seizing the Hidden Assassin: Current Detection Strategies for Staphylococcus aureus and Methicillin-Resistant S. aureus DOI
Qi Wang, Qingli Yang

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(30), P. 16569 - 16582

Published: July 20, 2024

(

Citations

7
Argonaute-Based Nucleic Acid Detection Technology: Advantages, Current Status, Challenges, and Perspectives DOI
Yaru Li, Lu Zhao,

Jiali Wang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(11), P. 5665 - 5682

Published: Nov. 11, 2024

Rapid and accurate detection is a prerequisite for precise clinical diagnostics, ensuring food safety, facilitating biotechnological applications. The Argonaute system, as cutting-edge technique, has been successfully repurposed in biosensing beyond the CRISPR/Cas system (clustered regularly interspaced short palindromic repeats CRISPR-associated proteins), which extensively researched, but recognition of PAM sequences remains restricted. Argonaute, programmable target-activated nuclease, fabricating novel methods due to its unparalleled biological features. In this comprehensive review, we initially elaborate on current nucleic acid testing nucleases, followed by delving into structure nuclease activity system. advantages compared with are highlighted discussed. Furthermore, summarize applications Argonaute-based provide an in-depth analysis future perspectives challenges. Recent research demonstrated that innovative rapidly advancing technology can overcome limitations existing potentially replace them. summary, implementation integration other technologies hold promise developing customized intelligent across various aspects.

Language: Английский

Citations

6