Sulfated Glycomimetic α-Helical Polypeptides for Antiviral Activity

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(7), P. 3115 - 3126

Published: June 14, 2023

In this work, we developed a library of sulfated glycomimetic polypeptides with high degree (up to 99%) via click reaction and sulfation modification, enabling control over the helicity, molecular weight, rigidity, side-chain structure. Their potentials as inhibitors SARS-CoV-2 common enterovirus were investigated, structure–activity relationship was explored in detail. The vitro results revealed crucial role α-helical conformation sugar since all glycopolypeptides exhibited outperformed activity suppressing infection inhibition efficiency up 85%. Other structural properties, including rigid chain structure moderate also contributed blocking viral entry into host cells. Among glycopolypeptides, L60-SG-POB showed highest an IC50 0.71 μg/mL. Furthermore, these optimized capable preventing 86%. This work opens new avenues for development synthetic bearing sugars against other viruses.

Language: Английский

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Potential and risks of nanotechnology applications in COVID-19-related strategies for pandemic control

Journal of Nanoparticle Research, Journal Year: 2023, Volume and Issue: 25(11)

Published: Nov. 1, 2023

Abstract The ongoing battle against viral infections highlighted so recently by the COVID-19 pandemic demonstrates need to develop new approaches using nanotechnology in antiviral strategies. Nanoparticles have emerged as promising tools fight outbreaks, offering various options for application such biosensors, vaccine nanoparticles, disinfectants, and functionalized nanoparticles. In this comprehensive review, we evaluate role of nanoparticles control, exploring their potential applications, benefits, associated risks. We first discuss importance outbreak management, particularly development. Although lipid play a crucial mRNA vaccines, there are concerns about side effects. functionalization protective face masks metallic has sustainable alternative disposable masks, reducing waste production enhancing virus filtration, improper disposal leads environmental contamination ecological harm. Second, address adverse effects with nanoparticle-based vaccines containing polyethylene glycol other components, which trigger autoimmune diseases alter menstrual cycles. To manage outbreaks effectively, must minimize risks impacts. Thus, when developing effective strategies future it is understand advantages challenges nanoparticle usage.

Language: Английский

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Pathogen‐binding nanoparticles to inhibit host cell infection by heparan sulfate and sialic acid dependent viruses and protozoan parasites

Smart Medicine, Journal Year: 2024, Volume and Issue: 3(2)

Published: March 1, 2024

Global health faces an immense burden from infectious diseases caused by viruses and intracellular protozoan parasites such as the coronavirus disease (COVID-19) malaria, respectively. These pathogens propagate through infection of human host cells. The first stage this cell mechanism is attachment, which typically involves interactions between agent surface components on membranes, specifically heparan sulfate (HS) and/or sialic acid (SA). Hence, nanoparticles (NPs) contain or mimic HS/SA that can directly bind to pathogen inhibit are emerging potential candidates for alternative anti-infection therapeutic strategy. NPs be prepared metals, soft matter (lipid, polymer, dendrimer), DNA, carbon-based materials among others designed include aspects multivalency, broad-spectrum activity, biocidal mechanisms, multifunctionality. This review provides overview anti-pathogen nanomedicines beyond drug delivery. Nanoscale inhibitors acting against obligate discussed. In future, availability broadly applicable nanotherapeutics would allow early tackling existing upcoming viral diseases. Invasion inhibitory could also provide urgently needed effective treatments parasitic infections.

Language: Английский

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Development of Nanomaterials as Drug Candidates

BENTHAM SCIENCE PUBLISHERS eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 66 - 107

Published: July 4, 2024

Nanomaterials, with their unique therapeutic traits such as antioxidant, antiinflammatory, antibacterial, antiviral, and anticancer properties, can be used drug candidates to treat a wide range of diseases. Nano complexes like dendrimers, carbon nanotubes, fullerenes, graphene-based nanomaterials, quantum dots, nanohydrogels, peptide nanostructures, MXenes, Silicene, Antimonene have been distinguished by researchers, among the many nanomaterials because lower toxicity, ease tuning desired end use, complex interactions biological macromolecules, solubility properties. This chapter will present most recent research details on that developed number illnesses.<br>

Language: Английский

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Dose-Response Effect of Various Concentrations of Cl-Containing Water-Soluble Derivatives of C60 Fullerenes on a Selective Regulation of Gene Expression in Human Embryonic Lung Fibroblasts (HELF)

Frontiers in Bioscience-Landmark, Journal Year: 2024, Volume and Issue: 29(10)

Published: Oct. 12, 2024

Background: The new synthesized water-soluble derivatives of C60 fullerenes are a great interest to researchers since they can potentially be promising materials for drug delivery, bioimaging, biosonding, and tissue engineering. Surface functionalization fullerene changes their chemical physical characteristics, increasing solubility suitability different biological systems applications, however, any in functionalized modulate cytotoxicity antioxidant properties. toxic or protective effect on cells is realized through the activation inhibition genes proteins key signaling pathways responsible regulation cellular reactive oxygen species (ROS) level, proliferation, apoptosis. Methods: 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was used assess viability. Flow cytometry analyses applied measure levels human embryonic lung fibroblasts (HELF) cells. HELF standard, stable well described cell line that passaged many times. Quantitation ROS assessed using H2DCFH-DA. Fluorescence images were obtained microscopy. Expression BCL2, CCND1, CDKN2A, BRCA1, BAX, NFKB1, NOX4, NRF2, TBP (reference gene) analyzed real-time Polymerase chain reaction (PCR). Results: We found high low concentrations with five residues potassium salt 6-(3-phenylpropanamido)hexanoic (F1) 6-(2-(thiophen-2-yl)acetamido)hexanoic (F2) acid chlorine atom attached directly cage cause diametrically opposite regulating level oxidative stress apoptosis HELF. High F1 F2 have genotoxic effect, causing NADPH oxidase 4 (NOX4) expression 24–72 hours (2–4 fold increase), synthesis induction (increase by 30–40%), DNA damage breaks (2–2.5 8-oxodG increases), nuclear factor kappa-light-chain-enhancer activated B (NF-κB) (by 40–80%) against background reduced NF-E2-related 2 (NRF2) 20–45%). Low produced cytoprotective effect: reduce 20–40%), decrease number double-strand 20–30%), increase anti-apoptotic enhance response activating NRF2 (NRF2 gene increases 1.5–2.3 fold, phosphorylated form protein 2–3 fold). Conclusions: Obtained results show doses studied fullrens may serve as perspective protectors damaging factors.

Language: Английский

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Fullerenol C60(OH)40 Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12221 - 12255

Published: Nov. 1, 2024

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the nasal cavity, penetrates epithelial cells through interaction of its spike protein with host cell receptor angiotensin-converting enzyme (ACE2) and then triggers a cytokine storm. We aimed to assess biocompatibility fullerenol nanoparticles C

Language: Английский

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