Revolutionary self-powered transducing mechanism for long-lasting and stable glucose monitoring: achieving selective and sensitive bacterial endospore germination in microengineered paper-based platforms

Microsystems & Nanoengineering, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 12, 2024

Abstract We introduce a groundbreaking proof-of-concept for novel glucose monitoring transducing mechanism, marking the first demonstration of spore-forming microbial whole-cell sensing platform. The approach uses selective and sensitive germination Bacillus subtilis spores in response to potassium-rich bodily fluids such as sweat. As rate number metabolically active germinating cells are directly proportional concentration, electrogenic activity these cells—manifested electricity—serves self-powered signal detection. Within microengineered, paper-based fuel cell (MFC), electrical power outputs measurable can be visually displayed through compact interface, providing real-time alerts. dormant extend shelf-life, self-replicating bacteria ensure robustness. MFC demonstrated remarkable sensitivity 2.246 µW·(log mM) −1 ·cm −2 concentrations ranging from 0.2 10 mM, with notably lower limit detection at ~0.07 mM. sensor exhibited exceptional selectivity, accurately detecting even presence various interferents. Comparative analyses revealed that, unlike conventional enzymatic biosensors whose performance degrades significantly time when inactive, spore-based is stable extended periods promptly regains functionality needed. This preliminary investigation indicates that strategy holds considerable promise efficient diabetes management toward noninvasive wearable monitoring, overcoming critical challenges current technologies paving way advanced biosensing applications.

Language: Английский

Integrating Renewable Microbial Fuel Cells in Dual In‐Line Package for Chip‐On‐Board Circuits

Advanced Materials Technologies, Journal Year: 2023, Volume and Issue: 8(22)

Published: Sept. 3, 2023

Abstract Single‐use electrical systems represent the future of multiple fields such as diagnostic medical technologies, environmental studies, and biofuel manufacturing with significant advantages over conventional unrecyclable bulky that are partly disposable at best. require miniaturized bio‐friendly energy sources meet recyclability or reusability requirement application without creating toxic waste. Herein, a storable, scalable, single‐use electronics‐compatible bio‐battery is designed fabricated using completely reusable recyclable components developed. The battery dual‐in‐line package microbial fuel cell (DIP‐MFC) can be activated on demand via introduction moisture through anodic fluid chamber. incorporates dormant bacteria cells an abiotic stainless steel mesh serves anode attracts electrons being transferred from biocatalyst. DIP‐MFC uses infestation bacterial biofilm conductive to harness deliver electricity selected circuit pins. A single continuously operates for 140 min maximum open voltage 0.55 V, which stacked connected match power requirements targeted electronics. DIP nature proposed allows simple integration MFC electronics boards

Language: Английский