Carboxymethyl chitosan/oxidized hyaluronic acid hydrogel-encapsulated hucMSC-derived exosomes for enhanced hepatic regeneration and post-hepatectomy applications

Carbohydrate Polymers, Journal Year: 2025, Volume and Issue: 353, P. 123248 - 123248

Published: Jan. 13, 2025

Language: Английский

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Dermal Fibroblast-Derived Exosomes Promotes Bone-to-Tendon Interface Healing of Chronic Rotator Cuff Tear in Rabbit Model

Arthroscopy The Journal of Arthroscopic and Related Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Advancements in extracellular vesicles biomanufacturing: a comprehensive overview of large-scale production and clinical research

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 19, 2025

Extracellular vesicles (EVs) are nano-sized, membranous structures secreted by cells into the extracellular space, have attracted considerable attention in field of biosciences for their role intercellular communication various physiological and pathological processes. Their ubiquitous presence bodily fluids cell-specific characteristics make them promising candidates as biomarkers. Additionally, ability to transport biological therapeutics across different barriers specific target underscores significant translational potential diagnostic therapeutic purposes. Significant progress has been achieved translation EVs research clinical applications, however, challenges persist large-scale production EVs, particularly areas scalable manufacturing, efficient isolation methods, drug loading techniques, advanced characterization technology. This review critically examines complex processes involved biogenesis explores recent developments production. By synthesizing knowledge from these fields, this aims provide a holistic perspective on evolving landscape its underscoring both accomplishments obstacles that lie ahead fully realizing biomedicine.

Language: Английский

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Leptin Enhances M1 Macrophage Polarization and Impairs Tendon-Bone Healing in Rotator Cuff Repair: A Rat Model

Clinical Orthopaedics and Related Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Background Rotator cuff tears are common, affecting more than 60% of individuals older 80 years, and they have been implicated in 70% patients with shoulder pain. M1 polarization-related inflammation has reported to be associated poor healing outcomes rotator injury, leptin, an adipokine, a potential activator inflammation. However, whether leptin affects repair remains unknown. Questions/purposes Using vitro cell experiments vivo rat tear model, we therefore asked: (1) Does promote the polarization macrophages vivo? (2) impair biomechanical strength, histologic structure tendon-bone interface, bone mineral density (BMD), or gait scenario? (3) by upregulating tumor necrosis factor (TNF) pathway? Methods The impact on macrophage was determined reverse transcription–polymerase chain reaction (RT-PCR), Western blot test, immunofluorescence staining. effect assessed model comparing group suture terms gait, tensile BMD. In experiments, 8-week-old male Sprague Dawley rats were used, adapting previously developed model. supraspinatus tendon resected from greater tuberosity bilaterally, then secured its anatomical footprint using transosseous single-row technique. total, 30 randomized into two groups (suture, leptin) drawing lots (15 each group). They at 2, 4, 8 weeks after surgery. group, 100 µL normal saline injected subacromial space deltoid muscle restitched original position. solution (200 ng/mL) Biomechanical properties including maximal failure load, stiffness, stress assess strength 4 Histologic staining conducted compare interface between treatment groups. Micro-MRI micro-CT assessments overall outcome BMD Gait analysis stride length Finally, explore underlying mechanism effects Necrostatin-1 (Nec-1) used block TNF signaling pathway study, RT-PCR mechanism. Results Leptin enhanced LPS-induced vitro, showing increased gene expression CD86, Nos2, TNF-α as well protein TNF-α, interleukin-6 (IL-6), inducible NO synthase (iNOS). showed that promoted. At 2 postoperatively, there cells (53 ± 5 versus 77 8, mean difference 24 [95% confidence interval (CI) 11 37]; p = 0.002), although proportion (ratio number total macrophages) not higher (18.6% 2.9% 21.5% 1.7%, CI -2.8% 8.7%]; 0.36). exhibited (31 50 6, 19 6 32]; 0.008) (16.4% 2.6% 23.0% 3.0%, 6.6% 0.8% 12.4%]; 0.03). impaired healing. both different load (12.7 1.6 N 12.4 1.8 N, -0.3 -2.6 1.8]; 0.91), stiffness (5.1 0.7 N/mm 4.6 0.8 N/mm, -0.5 -1.3 0.5]; 0.44), (2.0 0.3 2.0 , 0.0 -0.4 0.4]; 0.99). worse (17.6 1.4 14.1 -3.5 -5.7 -1.3]; (7.0 0.6 5.2 -1.8 -2.7 -0.9]; < 0.001), (3.4 2.8 0.4 -0.6 -1.0 -0.2]; 0.007). staining, image assessments, also demonstrated process. upregulated molecules pathway, CCL2 receptor-interacting kinase 1 (RIPK1), markers, such Nos2; addition Nec-1 neutralized polarization, reducing blocking RIPK. similar trends. Conclusion Based results this appears tear, upregulate polarization. Clinical Relevance Obesity fatty infiltration tears. adipose factor, explored. Future studies might focus developing novel approaches improve obesity targeting aid biomaterials.

Language: Английский

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Restoring tendon microenvironment in tendinopathy: Macrophage modulation and tendon regeneration with injectable tendon hydrogel and tendon-derived stem cells exosomes

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 152 - 169

Published: Jan. 22, 2025

Language: Английский

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Advances and challenges in biomaterials for tendon and enthesis repair

Bioactive Materials, Journal Year: 2025, Volume and Issue: 47, P. 531 - 545

Published: Feb. 20, 2025

Language: Английский

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Biomaterials that passively and actively target macrophages promote the regeneration of injured tissues

Biomedical Technology, Journal Year: 2024, Volume and Issue: 8, P. 17 - 49

Published: Oct. 1, 2024

Language: Английский

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Efficacy of adipose, bone marrow, and tendon stem cell-derived exosomes on tendon healing

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: unknown, P. 106844 - 106844

Published: March 1, 2025

Language: Английский

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Nanoparticle-Driven Tendon Repair: Role of Vasoactive Intestinal Peptide in Immune Modulation and Stem Cell Enhancement

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Tendon repair remains challenging owing to the limited capacity for endogenous repair. Vasoactive intestinal peptide (VIP) promotes bone tissue regeneration; however, its role in tendon unclear. In present study, we demonstrated that VIP stimulated M2 polarization of macrophages and facilitated regeneration by regulating immune homeostasis maintaining function stem/progenitor cells (TSPCs). Additionally, established GelMa-loaded VIP@PLGA@ZIF-8 (VPZ) nanoparticles (VPZG) enable sustained localized release at site patellar injury SD rats. The results vitro experiments VPZG regulated macrophage downregulating NF-κB axis. also promoted efferocytosis suppressed proinflammatory factors. enhanced tenogenic differentiation TSPCs when cocultured with macrophages. vivo, implanted injury, where it released sustainably slowly promote regeneration. This effect was achieved through downregulation expression levels various inflammatory factors, as well regulation local homeostasis. conclusion, our enhancing TSPC function. These findings suggest is a promising avenue clinical improvement treatment.

Language: Английский

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Revealing the molecular mechanism of Buzhong Yiqi Decoction for tendon bone healing on the basis of network pharmacology, molecular docking and experimental validation

Journal of Ethnopharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 119726 - 119726

Published: April 1, 2025

Language: Английский

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