Dual-Fluorescent Quantum Dot Nanobead-Based Lateral Flow Immunoassay for Simultaneous Detection of C-Reactive Protein and Procalcitonin DOI
Xinyue Tang, Wenwen Xia,

Huanxing Han

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 7659 - 7665

Published: Nov. 1, 2024

Simultaneous detection of C-reactive protein (CRP) and procalcitonin (PCT) at the point care is crucial for management infections in patients with inflammation critical settings. The challenge detecting high concentrations CRP alongside low PCT plasma from inflammatory has limited clinical application multiplexed immunoassays. Herein, we developed a lateral flow immunoassay (LFIA) that employs quantum dot nanobeads (QDNBs) varying sizes colors to enable simultaneous quantification human plasma. To extend dynamic range detection, combined QDNBs smaller particle antibodies, thereby increasing assay's reducing hook effect. At same time, stronger fluorescence emitted by these larger QDNBs, conjugation allows nanogram level, meeting demand sensitivity. results show this method can detect 0.1 3 mg/L limit 0.09 ng/mL, which on par clinically used methods. By employing dual-color dual-size QDNB labeling strategy, successfully achieved broad sensitivity one-step point-of-care rapid test.

Language: Английский

Ag-coated Au nanostar-Based Lateral Flow Immunoassay for Highly Sensitive Influenza A virus antibody Detection in Colorimetric and Surface-Enhanced Raman Scattering (SERS) modes DOI
Jingwen Li, Min Liu,

Jinyue Zhu

et al.

Talanta, Journal Year: 2024, Volume and Issue: 285, P. 127351 - 127351

Published: Dec. 6, 2024

Language: Английский

Citations

4
pH-sensitive oligopeptide magnetic mesoporous silica beads for deoxyribonucleic acid extraction DOI Creative Commons
Sihua Qian, Yi‐Ting Wang, Junjie Fan

et al.

Nanoscale Advances, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

pH-Sensitive oligopeptide magnetic mesoporous silica beads were successfully prepared by using large-pore dendritic colloids as templates for DNA extraction.

Language: Английский

Citations

0
Magnetic nanoprobe-enabled lateral flow assays in the applications of food safety and in vitro diagnostic DOI
Shipeng Gao,

Xueyun Zheng,

Junyong Zhu

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 534, P. 216588 - 216588

Published: March 9, 2025

Language: Английский

Citations

0
Dual readout biosensor based on AIE-type immunomagnetic ZIF-8: from sample pretreatment to multi-residue detection DOI
Shuai Zhang, Jincheng Xiong,

Boyan Sun

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137601 - 137601

Published: March 1, 2025

Language: Английский

Citations

0
Engineering an Ionic Aggregation-Induced Luminescence-Labeled Fluorescence Lateral Flow Immunoassay for C-Reactive Protein in Human Plasma DOI

Pei-Yuan Sun,

Q. Li, Ning Gao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 97(1), P. 565 - 573

Published: Dec. 27, 2024

The surge of lateral flow immunoassays (LFAs) stimulates researchers to explore the novel vibrant aggregation-induced emission luminogen (AIEgen)-doped nanoparticles improve accuracy and reliability LFAs. However, loading amount AIEgens currently used for LFA in microspheres is limited due their symmetrical large conjugated skeleton structure, which significantly reduces fluorescence brightness signal reporter LFA. Herein, an ionic with a donor–acceptor type was developed as C-reactive protein (CRP). Ionic are unable enter hydrophobic cavity polystyrene (PS) because low nature. By altering extended alkyl chains, it possible increase hydrophobicity, thereby potentially increasing capacity within PS. Notably, fluorescent (denoted AIETPANPs) formed by embedding (E)-4-(4-(diphenylamino)styryl)-1-octadecylpyridin-1-ium iodide (TPA) PS showed orange-red have high quantum yield. Anti-CRP antibody (mAb1) could be effectively surface AIETPANPs amino-carboxyl reaction, resulting AIETPANPs-mAb1. AIETPANPs-mAb1 exhibited at 613 nm, point detectable naked eye minimal background interference. entire analysis accomplished just 10 min, achieving limit detection 4.06 ng/mL CRP. AIETPANPs-mAb1-based demonstrates excellent stability specificity fully meets requirements clinical diagnosis.

Language: Английский

Citations

3
Lychee-shaped magneto-gold nanohybrid with highly retained magnetic-plasmonic activity for ultrasensitive and multiplex immunochromatographic assay DOI
Yuhao Wu,

Xiaolong Wang,

Jiayi Sun

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157262 - 157262

Published: Oct. 1, 2024

Language: Английский

Citations

1
Dual-Fluorescent Quantum Dot Nanobead-Based Lateral Flow Immunoassay for Simultaneous Detection of C-Reactive Protein and Procalcitonin DOI
Xinyue Tang, Wenwen Xia,

Huanxing Han

et al.

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(11), P. 7659 - 7665

Published: Nov. 1, 2024

Simultaneous detection of C-reactive protein (CRP) and procalcitonin (PCT) at the point care is crucial for management infections in patients with inflammation critical settings. The challenge detecting high concentrations CRP alongside low PCT plasma from inflammatory has limited clinical application multiplexed immunoassays. Herein, we developed a lateral flow immunoassay (LFIA) that employs quantum dot nanobeads (QDNBs) varying sizes colors to enable simultaneous quantification human plasma. To extend dynamic range detection, combined QDNBs smaller particle antibodies, thereby increasing assay's reducing hook effect. At same time, stronger fluorescence emitted by these larger QDNBs, conjugation allows nanogram level, meeting demand sensitivity. results show this method can detect 0.1 3 mg/L limit 0.09 ng/mL, which on par clinically used methods. By employing dual-color dual-size QDNB labeling strategy, successfully achieved broad sensitivity one-step point-of-care rapid test.

Language: Английский

Citations

1