Antioxidant and anti-inflammatory injectable hydrogel microspheres for in situ treatment of tendinopathy DOI Creative Commons
Qibin Han, Lang Bai,

Yinhua Qian

et al.

Regenerative Biomaterials, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 1, 2024

Tendinopathy is a common disorder that causes local dysfunction and reduces quality of life. Recent research has indicated alterations in the inflammatory microenvironment play vital role pathogenesis tendinopathy. Herein, injectable methacrylate gelatin (GelMA) microspheres (GM) were fabricated loaded with heparin-dopamine conjugate (HDC) hepatocyte growth factor (HGF). GM@HDC@HGF designed to balance by inhibiting oxidative stress inflammation, thereby regulating extracellular matrix (ECM) metabolism halting tendon degeneration. Combining factors heparin was expected improve encapsulation rate maintain long-term efficacy HGF. In addition, catechol groups on dopamine have adhesion antioxidant properties, allowing potential attachment at injured site, better function synergized injected

Language: Английский

In Situ Remodeling of Efferocytosis via Lesion‐Localized Microspheres to Reverse Cartilage Senescence DOI Creative Commons
Wei Xiong, Zeyu Han, Shenglong Ding

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(19)

Published: March 13, 2024

Abstract Efferocytosis, an intrinsic regulatory mechanism to eliminate apoptotic cells, will be suppressed due the delayed apoptosis process in aging‐related diseases, such as osteoarthritis (OA). In this study, cartilage lesion‐localized hydrogel microspheres are developed remodel situ efferocytosis reverse senescence and recruit endogenous stem cells accelerate repair. Specifically, aldehyde‐ methacrylic anhydride (MA)‐modified hyaluronic acid (AHM), loaded with pro‐apoptotic liposomes (liposomes encapsulating ABT263, A‐Lipo) PDGF‐BB, namely A‐Lipo/PAHM, prepared by microfluidic photo‐cross‐linking techniques. By a degraded porcine explant OA model, lesion location experiment illustrated that aldehyde‐functionalized promote affinity for cartilage. vitro data showed A‐Lipo induced of senescent chondrocytes (Sn‐chondrocytes), which can then phagocytosed macrophages, remodeling facilitated protection normal maintained chondrogenic differentiation capacity MSCs. vivo experiments confirmed localized reversed promoted repair OA. It is believed strategy great significance tissue regeneration diseases.

Language: Английский

Citations

15
Exploring precision treatments in immune‐mediated inflammatory diseases: Harnessing the infinite potential of nucleic acid delivery DOI Creative Commons
Lingxiao Xu, Z. Shao, Xia Fang

et al.

Exploration, Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Immune-mediated inflammatory diseases (IMIDs) impose an immeasurable burden on individuals and society. While the conventional use of immunosuppressants disease-modifying drugs has provided partial relief control, their inevitable side effects limited efficacy cast a shadow over finding cure. Promising nucleic acid have shown potential to exert precise at molecular level, with different classes acids having regulatory functions through varying mechanisms. For better delivery acids, safe effective viral vectors non-viral systems (including liposomes, polymers, etc.) been intensively explored. Herein, after describing range categories vectors, we focus application therapeutic in various IMIDs, including rheumatoid arthritis, bowel disease, psoriasis, multiple sclerosis, asthma, ankylosing spondylitis, systemic lupus erythematosus, uveitis. Molecules implicated inflammation immune dysregulation are abnormally expressed series meticulous modulation therapy results degrees remission improvement these diseases. By synthesizing findings centered specific targets, this review delivers systematic elucidation perspective towards advancing utilization therapeutics for managing IMIDs.

Language: Английский

Citations

13
Biomaterials for inflammatory bowel disease: treatment, diagnosis and organoids DOI
Jia Wang, Yuying Shi,

Bei Mao

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 36, P. 102078 - 102078

Published: Jan. 20, 2024

Language: Английский

Citations

9
Kirigami triboelectric spider fibroin microneedle patches for comprehensive joint management DOI Creative Commons
Shuhuan Li,

Suwen Cao,

Lu Huihui

et al.

Materials Today Bio, Journal Year: 2024, Volume and Issue: 26, P. 101044 - 101044

Published: April 4, 2024

Joint injuries are among the leading causes of disability. Present concentrations were focused on oral drugs and surgical treatment, which brings severe unnecessary difficulties for patients. Smart patches with high flexibility intelligent drug control-release capacity greatly desirable efficient joint management. Herein, we present a novel

Language: Английский

Citations

9
Bioinspired Hybrid Nanostructured PEEK Implant with Enhanced Antibacterial and Anti-inflammatory Synergy DOI
Ji Ao, Xin Zhang, Yunhao You

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(30), P. 38989 - 39004

Published: July 22, 2024

Implant-associated infections and excessive immune responses are two major postsurgical issues for successful implantation. However, conventional strategies including antibiotic treatment inflammatory regulation always compromised due to the comodification of various biochemical agents instances functional interference. It is imperative provide implant surfaces with satisfactory antibacterial anti-inflammatory properties. Here, a dual-effect nanostructured polyetheretherketone (PEEK) surface (NP@PDA/Zn) bionic mechano-bactericidal nanopillars immobilized immunomodulatory Zn2+ designed. The constructed hybrid display remarkable performance against Gram-negative Gram-positive strains through synergy physical chemical bactericidal effects imposed by Zn2+. Meanwhile, immunoregulatory property evaluated investigation macrophage polarization both in vitro vivo, results reveal that NP@PDA/Zn could downregulate expression M1-related cytokines decrease M1 recruitment lower response. Notably, exhibited exceptional biocompatibility discerning biocidal activity between bacterial mammalian cells antioxidant effectively scavenges ROS, minimizing potential cytotoxicity. Taken together, presents convenient promising strategy combining synergistic without any mutual interference, which can support development multifunctional implant-associated materials.

Language: Английский

Citations

9
Slide‐Ring Structured Stress‐Electric Coupling Hydrogel Microspheres for Low‐Loss Transduction Between Tissues DOI Open Access
Fan Wang, Xiaoyu Han, Zeyu Han

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

High transductive loss at tissue injury sites impedes repair. The high dissipation characteristics in the electromechanical conversion of piezoelectric biomaterials pose a challenge. Therefore, supramolecular engineering and microfluidic technology is utilized to introduce slide-ring polyrotaxane conductive polypyrrole construct stress-electric coupling hydrogel microspheres. molecular slippage mechanism structure stores releases mechanical energy, reducing loss, barium titanate enables stress-electricity conversion, conjugated π-electron movement network improves internal electron transfer efficiency microspheres, thereby for first time. Compared traditional low-dissipation microspheres increased by 2.3 times, energy decreased 43%. At cellular level, electrical signals generated triggered Ca

Language: Английский

Citations

1
Downregulation of the PI3K/AKT/mTOR/MMP-13 Pathway for Promoting Interface Healing via Lubricating Microspheres DOI

Shiyi Yao,

Hui Yuan,

Luqi Yang

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
In Situ Proefferocytosis Microspheres as Macrophage Polarity Converters Accelerate Osteoarthritis Treatment DOI Open Access
Yong Wang, Chaoyu Pu, Zeyu Han

et al.

Small, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Efferocytosis in macrophages typically engages an anti‐inflammatory positive feedback regulatory mechanism. In osteoarthritis (OA), characterized by imbalanced inflammatory homeostasis, the proinflammatory state of immune microenvironment can be reversed through enhanced efferocytosis. This study develops situ proefferocytosis hydrogel microsphere (macrophage polarity converter, H‐C@IL) for OA treatment. Immunoliposomes (IL), CD16/32 antibody‐modified clodronate liposomes, are initially prepared using Re‐emulsion method. Then, IL is loaded into CCL19‐modified HAMA microspheres microfluidic technology. vitro, H‐C@IL specifically recruit M0 and M1 via CCL19, induce apoptosis secondary targeting with IL, provide “Find/Eat‐me” signals to enhance Additionally, it promotes macrophage polarization toward M2 phenotype. vivo, behavioral, imaging, histological analyses demonstrate that effectively facilitates M2, inhibits inflammation, cartilage regeneration. Mechanistically, enhances efferocytosis activating proteins such as PROS1 TIMD4 macrophages. Concurrently, signaling pathways, including PQLC2‐Arg‐Rac1 Pbx1/IL‐10, activated drive from M2. summary, situ, restores regeneration, offering a comprehensive treatment strategy OA.

Language: Английский

Citations

1
Injectable Chondroitin Sulfate Microspheres with Gallic Acid-Magnesium MOF for Anti-Inflammatory and Cartilage Degeneration Alleviation in Osteoarthritis Treatment DOI
Jiachen He, Jian Wu,

Jingcheng Zheng

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Inflammation and cartilage degeneration are critical challenges in osteoarthritis (OA) treatment. Achieving sustained drug efficacy while mitigating the adverse effects of inflammation reactive oxygen species remains a significant challenge. This study synthesizes gallic acid-magnesium (GA-Mg) metal–organic framework (MOF) as carrier for puerarin (PA). The PA-loaded GA-Mg MOF (pGM) is encapsulated within chondroitin sulfate methacrylate, forming monodisperse hybrid microspheres (CM@pGM) under ultraviolet light using microfluidic technology. pGM physically confined through network structural obstructions noncovalent interactions. During degradation, GA Mg2+ ions release from pGM, improving inflammatory microenvironment articular cavity oxidative stress. PA supports chondrocyte anabolism facilitates repair. In vitro studies confirm that injectable extend period to over 2 weeks. vivo experiments demonstrate CM@pGM significantly reduces osteophyte formation, alleviates degenerative changes cartilage, delays OA progression. conclusion, CM@pGM, delivery platform ameliorates microenvironment, stress, promotes repair, holds potential

Language: Английский

Citations

1
Quercetin-solid lipid nanoparticle-embedded hyaluronic acid functionalized hydrogel for immunomodulation to promote bone reconstruction DOI Creative Commons
Pinghui Zhou,

Bomin Yan,

Bangguo Wei

et al.

Regenerative Biomaterials, Journal Year: 2023, Volume and Issue: 10

Published: Jan. 1, 2023

Bone defects are a persistent challenge in clinical practice. Although repair therapies based on tissue-engineered materials, which known to have crucial role defective bone regeneration, gathered increased attention, the current treatments for massive several limitations. In present study, immunomodulatory inflammatory microenvironment properties of quercetin, we encapsulated quercetin-solid lipid nanoparticles (SLNs) hydrogel. Temperature-responsive poly(ε-caprolactone-co-lactide)-b-poly(ethylene glycol)-b-poly(ε-caprolactone-co-lactide) modifications were coupled main chain hyaluronic acid hydrogel, constructing novel, injectable hydrogel scaffold. Extensive

Language: Английский

Citations

20