Langmuir,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Copolymer
nanovesicles
are
used
extensively
in
chemical
processes
and
biomedical
applications
which
they
subjected
to
dynamic
flow
environments.
Flow-induced
vesicle
deformation,
fragmentation,
reorganization
modify
the
energetic
(e.g.,
polymer-solvent
interfacial
area)
entropic
copolymer
chain
configuration)
contributions
solution
free
energy.
Equilibration
of
a
deformed
morphology
by
cessation
could
reorganize
system
into
self-assembled
state,
is
different
from
parent
structure
through
local
energy
minimization
pathway.
We
perform
nonequilibrium
molecular
dynamics
simulations
investigate
evolution
uniform
shear
unilamellar
nanovesicle
formed
self-assembly
amphiphilic
triblock
copolymers
an
aqueous
solution.
Flow
strength
characterized
Weissenberg
number
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 16, 2025
Abstract
The
design
and
assembly
of
protocell
models
that
can
mimic
the
features
functions
life
present
a
significant
research
challenge
with
potential
for
far‐reaching
impact.
Inspired
by
natural
phenomenon
microbe‐induced
mineralization,
way
is
developed
to
induce
spontaneous
formation
mineralized
membrane
on
surface
coacervate
droplets
utilizing
Fe
3+
ions.
In
particular,
effect
ions
microstructure
at
molecular
level
dissected
combining
theoretical
experimental
approaches.
reversible
process
be
regulated
redox
reactions
involving
2+
/Fe
within
coacervate.
not
only
enhances
stability
prevents
aggregation
coalescence,
but
also
allows
adjacent
together.
membranized
assemblages
retain
inherent
properties
biomolecule
sequestration
enzyme
catalysis,
demonstrate
excellent
resistance
high
temperatures
pressures
as
well
good
over
30
days.
This
study
will
offer
new
platform
coacervate‐based
life‐like
biomimetic
systems,
enhance
understanding
interactions
underlying
various
biological
phenomena
level.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 9, 2025
The
stimulus-response
behavior
of
protocells
under
environmental
osmotic
pressure
changes
has
long
been
a
subject
scientific
inquiry.
Herein,
we
demonstrate
way
to
membranized
coacervate
microdroplets
based
on
cholesterol
anchoring
phospholipids,
which
provides
enhanced
stability,
enabling
morphological
transformations
instead
dissociation
during
subsequent
changes.
In
hypotonic
environments,
these
coacervates
equilibrate
through
transient
internal
vacuole
formation,
concomitant
with
transmembrane
substrate
influx
that
triggers
enzymatic
reaction
acceleration.
By
contrast,
in
hypertonic
environment,
the
responds
bursting-like
deformation
can
then
quickly
recover
due
effect
phospholipids.
Notably,
it
is
found
such
could
even
successfully
induce
endocytosis
Staphylococcus
aureus
by
coacervates.
Furthermore,
integration
Coa@DMPC's
responsiveness,
actin
polymerization
activated
endocytic
S.
achieved.
Not
only
our
proposed
method
phospholipid
membranization
contribute
new
model
mimic
more
complex
bionic
structures,
but
also
revealed
response
various
expected
help
explain
stress
behaviors
and
emerging
unique
properties
cells
similar
environments.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Oct. 2, 2024
Oil-in-water
emulsion
is
a
system
with
extensive
applications
in
foods,
cosmetics
and
coating
industries,
it
could
also
be
designed
into
an
artificial
lipid
droplet
recent
works.
However,
the
insights
biophysical
dynamic
behaviors
of
such
droplets
are
lacking.
Here,
we
reveal
enzymatic
reaction
triggered
endocytosis-like
behavior
oil-in-water
droplets.
A
thermodynamically
favored
recruitment
lipases
onto
membrane
demonstrated.
We
confirm
that
hydrolysis
tributyrin
by
can
decrease
interfacial
tension
increase
compressive
force
on
membrane,
which
two
main
driving
forces
for
triggering
behavior.
The
induced
various
emerging
functionalities
droplets,
including
proteins,
DNA
or
inorganic
particles
being
efficiently
sequestered
oil
reversible
release
as
well
enhanced
cascade
reaction.
Overall,
our
studies
expected
to
open
up
way
functionalize
emulsions
capable
life-inspired
tackle
challenges
bottom-up
synthetic
biology,
revealing
unknown
living
organisms.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 26, 2024
As
the
foundation
of
life,
a
cell
is
generally
considered
an
advanced
microreactor
with
complicated
structure
and
function.
Undeniably,
this
fascinating
complexity
motivates
scientists
to
try
extricate
themselves
from
natural
living
matter
work
toward
rebuilding
artificial
cells
in
vitro.
Driven
by
synthetic
biology
bionic
technology,
research
has
gradually
become
subclass.
It
not
only
held
import
many
disciplines
but
also
great
interest
its
synthesis.
Therefore,
review,
we
have
reviewed
development
strategies
focused
on
efforts
bottom-up
construction.
Different
starting
existing
organisms,
discussed
construction
based
biomimetic
materials,
simple
scaffolds
multiple
compartment
systems,
functional
modules
simulation
crucial
metabolism
behaviors,
or
even
communication
networks.
All
them
could
represent
exciting
advance
field.
In
addition,
will
make
rough
analysis
bottlenecks
Meanwhile,
future
field
been
prospecting.
This
review
may
bridge
gap
between
materials
engineering
life
sciences,
forming
theoretical
basis
for
developing
various
life-inspired
assembly
materials.
Biomacromolecules,
Journal Year:
2023,
Volume and Issue:
24(12), P. 5749 - 5758
Published: Nov. 7, 2023
Modulated
membrane
functionalization
is
a
necessary
and
overarching
step
for
hollow
microcompartments
toward
their
application
as
nanoreactors
or
artificial
cells.
In
this
study,
we
show
way
to
generate
phospholipid
hybrid
proteinosomes
that
could
superposed
virtues
of
liposomes
proteinosomes.
comparison
pure
proteinosomes,
both
the
fluidity
permeability
are
improved
obviously
after
forming
Specifically,
integration
phospholipids
also
endows
demonstrating
stepwise
release
encapsulants
FITC-dextran
(70
150
kDa)
triggered
sequentially
by
phospholipase
protease,
then
modulated
cascaded
enzymatic
reaction
between
two
different
populations
achieved.
Therefore,
it
anticipated
such
constructed
be
employed
an
microcompartmental
model
further
advanced
cell
design
achieving
logic
signal
communication
within
various
cellular
well
potential
applications
in
field
microreactors.
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
25(9), P. 6127 - 6134
Published: Aug. 6, 2024
We
present
a
straightforward
strategy
for
constructing
giant,
multicompartmentalized
vesicles
using
recombinant
fusion
proteins.
Our
method
leverages
the
self-assembly
of
globule-zipper-elastin-like
polypeptide
protein
complexes
in
aqueous
conditions,
eliminating
need
organic
solvents
and
chemical
conjugation.
By
employing
thin-film
rehydration
method,
we
have
successfully
encapsulated
diverse
range
bioactive
macromolecules
engineered
organelle-like
compartments─ranging
from
soluble
proteins
coacervate
droplets
to
vesicles─within
these
protein-assembled
giant
vesicles.
This
approach
also
facilitates
integration
water-soluble
block
copolymers,
enhancing
structural
stability
functional
versatility
results
suggest
that
multicompartment
not
only
mimic
complex
architecture
living
cells
but
support
biochemically
distinct
reactions
regulated
by
functionally
folded
proteins,
providing
robust
model
studying
cellular
processes
designing
microreactor
systems.
work
highlights
transformative
potential
self-assembling
artificial
cell
design.
Chemical Communications,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Jan. 1, 2024
This
review
summarizes
recent
advancements
in
the
study
of
multiphase
coacervates,
focusing
on
design
strategies,
underlying
mechanisms,
structural
control,
and
their
applications
biomimetics.
ChemSystemsChem,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 15, 2024
Abstract
Coacervates
have
been
investigated
as
protocells
or
synthetic
cells,
well
subcellular
compartments
for
the
creation
of
new
materials,
thus
bridging
gap
between
living
and
non‐living
systems
in
materials
science,
biology,
bioengineering.
Given
design
flexibility
simplicity
coacervates,
along
with
functionality
complexity
natural
interfacing
complex
coacervates
cells
is
considered
significant
various
biotechnological
biomedical
applications.
In
this
review,
fundamental
mechanisms
underlying
theories
coacervate
are
introduced.
Recent
efforts
to
interface
summarized
three
key
scenarios:
(i)
integration
cell
components
material
assembly
into
protocells;
(ii)
communication
therapeutic
drug
delivery
repair;
(iii)
formation
intracellular
condensates
metabolic
regulation,
followed
by
regulation
their
phase
transitions
pathological
elucidation.
Finally,
potential
coacervate‐natural
interfaces
discussed
context
developing
living/synthetic
constructs,
creating
precise
disease
therapy
strategies,
advancing
programmable
engineering
networks.
Langmuir,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 31, 2024
Copolymer
nanovesicles
are
used
extensively
in
chemical
processes
and
biomedical
applications
which
they
subjected
to
dynamic
flow
environments.
Flow-induced
vesicle
deformation,
fragmentation,
reorganization
modify
the
energetic
(e.g.,
polymer-solvent
interfacial
area)
entropic
copolymer
chain
configuration)
contributions
solution
free
energy.
Equilibration
of
a
deformed
morphology
by
cessation
could
reorganize
system
into
self-assembled
state,
is
different
from
parent
structure
through
local
energy
minimization
pathway.
We
perform
nonequilibrium
molecular
dynamics
simulations
investigate
evolution
uniform
shear
unilamellar
nanovesicle
formed
self-assembly
amphiphilic
triblock
copolymers
an
aqueous
solution.
Flow
strength
characterized
Weissenberg
number
