Sensitivity to photoperiod is a complex trait in Camelina sativa DOI Creative Commons
Bryan A. Ramirez‐Corona, Eric A. Seagren, Carissa Sherman

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Summary Day neutrality, or insensitivity to photoperiod (day length), is an important domestication trait in many crop species. Although the oilseed Camelina sativa has been cultivated since Neolithic era, day-neutral accessions have yet be described. We sought leverage genetic diversity existing germplasms identify C. with low sensitivity for future engineering of this trait. quantified variation response across 161 by measuring hypocotyl length four-day-old seedlings grown long-day and short-day conditions, finding wide response. Similarly, soil-grown adult plants from selected showed several traits; however, responses seedling traits were not correlated, suggesting complex mechanistic underpinnings. RNA-seq experiments reference accession Licalla identified differentially regulated Arabidopsis syntelogs involved response, including COL2, FT, LHY WOX4 , expression these genes did correlate differences their sensitivity. Taken together, we show that all tested some degree likely complex, involving separable traits. Significance Statement neutrality (photoperiod insensitivity) a common domesticated crops; ancient remained photoperiod-sensitive, which limits seed yields. Here, conserved cultivars, albeit different degrees, establish trait, will require achieve day neutrality.

Language: Английский

Genetic Improvement of Camelina sativa (L.) Crantz: Opportunities and Challenges DOI Creative Commons
Martina Ghidoli, Elena Ponzoni, Fabrizio Araniti

et al.

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 570 - 570

Published: Jan. 27, 2023

In recent years, a renewed interest in novel crops has been developing due to the environmental issues associated with sustainability of agricultural practices. particular, cover crop, Camelina sativa (L.) Crantz, belonging Brassicaceae family, is attracting scientific community's for several desirable features. It related model species Arabidopsis thaliana, and its oil extracted from seeds can be used either food feed, or industrial uses such as biofuel production. From an agronomic point view, it grow marginal lands little no inputs, practically resistant most important pathogens Brassicaceae. Although cultivated past, particularly northern Europe Italy, last century, was abandoned. For this reason, breeding work conducted improve plant, also because low genetic variability present hexaploid species. review, we summarize main works on focused improvement three objectives: yield, seed content quality, reduction glucosinolates seed, which are anti-nutritional substances camelina. We report latest advances utilising classical plant breeding, transgenic approaches, CRISPR-Cas9 genome-editing.

Language: Английский

Citations

27
Dissecting the molecular basis of variability for flowering time in Camelina sativa DOI Creative Commons
Liyong Zhang,

Venkatesh Bollina,

Peng Gao

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Camelina sativa is an important polyploid oilseed crop with multiple favourable agronomic traits. Capturing the leaf transcriptome of 48 accessions C. suggests allelic variation for gene expression levels and notably sub-genome dominance, both which could provide opportunities improvement. Flowering time (FT) a crucial factor affecting overall yield crops. However, our understanding molecular mechanisms underlying FT regulation in are still limited, partly due to its complex allohexaploid genome. In this study, weighted co-expression network analysis (WGCNA), quantitative trait loci (eQTL) transcriptome-wide association study (TWAS) were employed explore diversity among dissect basis. Our results revealed FT-related co-expressed module highly enriched SOC1 SOC1-like genes identified 10 significant FT-associated single nucleotide polymorphisms (SNPs) defining three haplotype groups; thus providing basis future genetic improvements breeding.

Language: Английский

Citations

0
Molecular and archaeological evidence on the geographical origin of domestication for Camelina sativa DOI Creative Commons
Jordan R. Brock, Melissa M. Ritchey, Kenneth M. Olsen

et al.

American Journal of Botany, Journal Year: 2022, Volume and Issue: 109(7), P. 1177 - 1190

Published: June 18, 2022

Camelina (gold-of-pleasure or false flax) is an ancient oilseed crop with emerging applications in the production of sustainable, low-input biofuels. Previous domestication hypotheses suggested a European western Asian origin, yet little genetic evidence has existed to assess geographical origin for this crop, and archaeological data have not been systematically surveyed.We utilized genotyping-by-sequencing 185 accessions C. sativa its wild relatives examine population structure within species relationship populations progenitor, microcarpa; cytotype variation was also assessed both species. In complementary analysis, we surveyed literature identify sites archaeobotanical camelina remains timing prevalence usage across Europe Asia.The majority microcarpa sampled United States belongs variant (2n = 38) distinct evolutionary from that lineage 40). Populations Transcaucasia (South Caucasus) are most closely related based on structure; combination insights, these refute prior origin.Our findings support Caucasus, potentially Armenian, domestication. We cannot definitively determine whether intentionally targeted own right instead arose secondarily through selection agricultural traits weedy sativa, as originally proposed by Vavilov

Language: Английский

Citations

18
Sensitivity to Photoperiod Is a Complex Trait in Camelina sativa DOI Creative Commons
Bryan A. Ramirez‐Corona, Eric A. Seagren, Carissa Sherman

et al.

Plant Direct, Journal Year: 2025, Volume and Issue: 9(4)

Published: April 1, 2025

ABSTRACT Day neutrality, or insensitivity to photoperiod (day length), is an important domestication trait in many crop species. Although the oilseed C. sativa has been cultivated since Neolithic era, day‐neutral accessions have yet be described. We sought leverage genetic diversity existing germplasms identify with low sensitivity for future engineering of this trait. To do so, we quantified variation hypocotyl length across 161 4‐day‐old seedlings grown long‐day and short‐day conditions as a high‐throughput approximation response. Soil‐grown adult plants from selected also showed response day several traits; however, responses seedling traits were not correlated, suggesting complex mechanistic underpinnings. RNA‐seq experiments reference accession Licalla identified differentially regulated Arabidopsis syntelogs involved development, including COL2 , FT LHY WOX4, expression these genes did correlate differences their sensitivity. Taken together, show that all tested some degree likely complex, involving separable traits.

Language: Английский

Citations

0
The potential of integrative phenomics to harness underutilized crops for improving stress resilience DOI Creative Commons
Dominik K. Großkinsky, Jean‐Denis Faure, Yves Gibon

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: June 20, 2023

OPINION article Front. Plant Sci., 20 June 2023Sec. Crop and Product Physiology Volume 14 - 2023 | https://doi.org/10.3389/fpls.2023.1216337

Language: Английский

Citations

9
Constitution of a Camelina sativa L. Synthetic Population and Agronomic Comparison between Spring and Winter Cultivation in North Italy DOI Creative Commons
Martina Ghidoli, Sara Frazzini, Stefano De Benedetti

et al.

Agronomy, Journal Year: 2023, Volume and Issue: 13(6), P. 1562 - 1562

Published: June 7, 2023

In recent years, the interest in increasingly sustainable agriculture has also turned attention towards new cover crops suitable for use marginal areas that could enter food chain as protein and oil sources or biodiesel production. this scenario, Camelina sativa is a perfect crop to study. an annual herbaceous plant belonging Brassicaceae which interesting terms of its content, since seeds contain about 40% oils, with high level polyunsaturated fatty acids (30–40% alpha linolenic acid, 15–25% linoleic 15% oleic acid eicosenoic acid). It hexaploid species (2n = 40, genome size ~782 Mb) characterized by rapid growth, short life cycle (85–100 days spring varieties, 190–210 autumn varieties) low input cultivation needs. However, feed limited presence glucosinolates (GLS). GLS are sulfur molecules involved defense. they have been studied not only antinutritionals but their anti-carcinogenic effects against chronic inflammatory heart diseases natural pesticides. Given camelina highly nutritious oil, eight pure lines synthetic population were compared two different growing periods, winter. work, genetic materials phenotypic traits, yields yield components, bromatological glucosinolate content. The results confirmed North Italy, higher if cultivated autumn–winter period (about 2 t/ha vs. 0.6 t/ha); furthermore, negative correlation was found between winter yields, indicating varieties produce more less cultivation. Moreover, our knowledge, it first work tested proved be valid solution various environments both adaptability content 17 mmol/kg).

Language: Английский

Citations

7
The identification of the missing maternal genome of the allohexaploid camelina (Camelina sativa) DOI
Terezie Mandáková, Martin A. Lysák

The Plant Journal, Journal Year: 2022, Volume and Issue: 112(3), P. 622 - 629

Published: Aug. 2, 2022

SUMMARY Hexaploid camelina ( Camelina sativa ; 2 n = 6 x 40) is an important oilseed crop closely related to Arabidopsis. Compared other polyploid crops, the origin of three subgenomes has begun be unveiled only recently. While phylogenomic studies identified diploid C. hispida (2 14) as paternal genome , maternal donor remained unknown. Because chromosomes assigned a putative resembled those neglecta 12), tetraploid ‐like 4 26) was hypothesized likely ancestor hexaploid crop. Here we report chromosome‐level structure predicted among genotypes previously classified together microcarpa and referred here intermedia . Detailed cytogenomic analysis revealed high collinearity with two maternally inherited The identification missing provides new insights into reticulate evolutionary history complex allows us postulate comprehensive model for genus. herein elucidated opens door subsequent modifications resynthesis allohexaploid genome.

Language: Английский

Citations

12
New Flowering and Architecture Traits Mediated by Multiplex CRISPR-Cas9 Gene Editing in Hexaploid Camelina sativa DOI Creative Commons

Yannick Bellec,

Anouchka Guyon‐Debast, Tracy François

et al.

Agronomy, Journal Year: 2022, Volume and Issue: 12(8), P. 1873 - 1873

Published: Aug. 9, 2022

Adapting plants to sustainable cropping systems is a major challenge for facing climate change and promoting agroecological transition. Camelina sativa an emerging oilseed crop species with climate-resilient properties that could be used in double-cropping systems, particular as summer catch crop. The availability of early-flowering camelina essential such allow full completion the growth cycle during summer. Targeted induced gene variation (TIGV) was on several flowering repressor genes identified Arabidopsis obtain lines. Multiplex editing 15 target representing FLOWERING LOCUS C, SHORT VEGETATIVE PHASE, LIKE HETEROCHROMATIN PROTEIN 1, TERMINAL FLOWER 1 EARLY 3 combinatorial mutations were screened phenotypes. Certain mutants showing stable trait after five generations also presented additional phenotypes: determinate flowering, shorter stature and/or basal branching. Different combinations had positive or negative impact yield. This work demonstrates efficient multiplex CRISPR achievable hexaploid like camelina, providing valuable genetic diversity better selecting lines adapted new systems.

Language: Английский

Citations

12
Heat stress during reproductive stages reduces camelina seed productivity and changes seed composition DOI Creative Commons

Brian E. Smith,

Chaofu Lu

Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e26678 - e26678

Published: Feb. 1, 2024

Camelina (Camelina sativa L. Crantz) is a low-input oilseed crop with great potential in bioenergy and industrial oils. Improving tolerance to high temperatures essential for camelina agronomic sustainability. Two genotypes, Suneson Pryzeth, were exposed transient 14-day heat stress at 37 °C during the reproductive stages. Four cohorts of pods along main stem, which different stages from fully developed (C1), young (C2), open flowers (C3) flowering buds (C4) time treatment, examined morphological seed quality traits maturity. The stem length was shortened both genotypes. Pods seeds all negatively affected by heat, resulting lower yield reduced oil content. Seed size weight had greatest reduction C1, pod found most C3, number fertile that contain least one C3 C4. These results suggest effects are developmental stage specific. Heat significantly fertility inhibited storage product biosynthesis accumulation filling resulted smaller lighter seeds. Analyzing composition indicated content decreased while protein increased treated plants. In addition, fatty acid altered omega-3 α-linolenic concomitantly omega-6 linoleic being affected. Our also revealed responses two genotypes examined, suggesting genetic variation germplasm can be explored improve tolerance. This study provides resources guidance future studies understand physiological mechanisms assist improving sustainability production facing climate change.

Language: Английский

Citations

2
Genetic study of Camelina sativa oilseed crop and selection of a new variety by the bulk method DOI Creative Commons
Martina Ghidoli, Filippo Geuna, Stefano De Benedetti

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 28, 2024

Camelina sativa , commonly referred to as camelina or false flax, has emerged a promising cover crop with the potential mitigate climate change—a pressing global challenge that demands urgent and sustainable solutions. Belonging Brassicaceae family native Europe Central Asia, is an oilseed known for its resilience in diverse climates, including arid semi-arid regions, making it adaptable various environments. A breeding program started from study of six winter varieties five spring described: these genetic materials were characterized by SSRs molecular markers GBS technique. Molecular data clearly showed all genetically similar distinguishable varieties, which, turn, clustered together. Using data, parental belonging two different clusters selected generate new variability. The variety obtained, through bulk method based on three parameters: yield, earliness, weight 1000 seeds, allowed generation material provisionally named C1244. Chemical characterization was performed (bromatological glucosinolates analysis) better describe C1244 comparison benchmark varieties. exhibited early maturity, this use intercropping systems, high seeds (1.46 g) which improves facilitates seeding/harvesting operations oil content (33.62%) akin valuable human animal food purposes.

Language: Английский

Citations

2