Sericin-Assisted Green Synthesis of Gold Nanoparticles as Broad-Spectrum Antimicrobial and Biofilm-Disrupting Agents for Therapy of Bacterial Infection

International Journal of Nanomedicine, Journal Year: 2025, Volume and Issue: Volume 20, P. 3559 - 3574

Published: March 1, 2025

Tens of millions people die from wound infections globally each year, and nearly 80% tissue are associated with bacterial biofilms. However, overuse antibiotics can lead to resistance. Therefore, it is critical develop simple effective strategies kill bacteria remove The present study used sericin as a reducing stabilizing agent synthesize sericin-gold nanoparticles (Ser-Au NPs) tested its colloidal stability under different pH salt concentration conditions. Subsequently, functional gold nanocomposites (Ser-Au@MMI) were synthesized by combining Ser-Au NPs 2-mercapto-1-methylimidazole (MMI). antimicrobial effect Ser-Au@MMI was checked MIC, activity test, in vitro cytotoxicity assessed using CCK-8 assay. In anti-biofilm observed fluorescence microscopy SEM. Finally, the anti-infective therapeutic efficacy determined an vivo rat-infected model. Sericin exhibited excellent TEM, EDS, XPS analyses confirmed successful synthesis Ser-Au@MMI. It higher antibacterial due synergistic MMI AuNP, which achieve bactericidal destroying integrity cell walls structure. addition, Ser-Au@MMI10 (HAuCl4:MMI =1:10) (64 μg/mL) could effectively disrupt biofilms formed four species them, including P. aeruginosa, B. subtilis, E. coli, S. aureus, but not cytotoxic mouse fibroblasts (L929) cells. Infected modeling showed that accelerated infected healing vivo. prepared through facile environmentally friendly strategy have advantages low toxicity, has potential for application broad-spectrum biofilm disrupting healthcare.

Language: Английский

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Understanding the Role of Bacterial Biofilm in Antibiotic Resistance: Defensive Strategies and Clinical Challenges

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Bacterial biofilms significantly cause persistent exacerbation of infections in the clinical setting. These groups microorganisms are highly resistant to host immune responses and conventional antibiotic therapies, whereas they embedded an extracellular matrix. This chapter provides more detailed information on mechanism biofilm formation involving key stages adherence, maturation, spread, including composition structure a further explores how contribute resistance, physical barriers drug penetration, quorum sensing mechanisms, adaptive resistance strategies such as genetic adaptation, stress responses, persister cells. The role horizontal gene transfer spread within communities is also discussed. discusses challenges posed by biofilm-associated infections, focusing diagnosing treating chronic recurrent factors persistence, limitations current therapeutic options. Finally, we address emerging countermeasures counter mediated biofilms, enzymatic nanomedicine technologies, natural product-based inhibitors, inhibitory agents, photodynamic sonodynamic antimicrobial therapy, combinatorial therapies.

Language: Английский

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Advanced Antibacterial Strategies for Combatting Biomaterial‐Associated Infections: A Comprehensive Review

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Journal Year: 2024, Volume and Issue: 16(6)

Published: Nov. 1, 2024

ABSTRACT Biomaterial‐associated infections (BAIs) pose significant challenges in modern medical technologies, being a major postoperative complication and leading cause of implant failure. These significantly risk patient health, resulting prolonged hospitalization, increased morbidity mortality rates, elevated treatment expenses. This comprehensive review examines the mechanisms driving bacterial adhesion biofilm formation on biomaterial surfaces, offering an in‐depth analysis current antimicrobial strategies for preventing BAIs. We explore antimicrobial‐eluting biomaterials, contact‐killing antifouling coatings, emphasizing application polymer brushes devices. Recent advancements multifunctional which integrate multiple superior protection against BAIs, are also discussed. By evaluating advantages limitations these strategies, this aims to guide design development highly efficient biocompatible biomaterials. highlight potential routes that facilitate transition from laboratory research clinical applications. Additionally, we provide insights into synthetic biology as novel approach combat resistance. aspires inspire future innovation, ultimately improving outcomes advancing device technology.

Language: Английский

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Antimicrobial and Anti-Biofilm Effects of Dihydroartemisinin-loaded chitosan nanoparticles Against Methicillin-Resistant Staphylococcus aureus

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 107208 - 107208

Published: Dec. 1, 2024

Language: Английский

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