Journal of Photochemistry and Photobiology B Biology,
Journal Year:
2022,
Volume and Issue:
237, P. 112590 - 112590
Published: Oct. 29, 2022
Photobiomodulation
(PBM)
has
been
known
to
facilitate
the
healing
of
numerous
ailments
including
diabetic
wounds.
PBM
is
not
broadly
acknowledged
largely
due
scepticism
regarding
its
mechanism
action,
specific
molecular
targets
and
effects
rendered
at
a
tissue,
cellular
level.
Diabetes
mellitus
(DM)
reduces
signalling,
phosphatidylinositol
3
kinase/protein
kinase
B
(PI3K/AKT)
signalling
pathway
that
critical
during
wound
healing.
In
DM,
chronicity
common
may
lead
non-traumatic
limb
amputation.
This
study
investigated
hypothesis
660
nm
activates
PI3K/AKT
downstream
proteins
in
fibroblast
cells
vitro.
Cells
were
incubated
for
24
48
h
after
(660
5
J/cm2),
followed
by
evaluation
activated
mammalian
target
rapamycin
(mTOR)
glycogen
synthase
kinase-3
beta
(GSK3β)
using
western
blotting
ELISA.
Irradiated
cell
models
displayed
increased
activation
mTOR
GSK3β
proteins.
Our
findings
suggest
therapeutic
stressed
are
through
provides
novel
understanding
into
one
probable
mechanisms
which
wavelength
(5
J/cm2)
enhances
activities
Frontiers in Bioengineering and Biotechnology,
Journal Year:
2022,
Volume and Issue:
10
Published: Jan. 25, 2022
Tissue
repair
after
trauma
and
infection
has
always
been
a
difficult
problem
in
regenerative
medicine.
Hydrogels
have
become
one
of
the
most
important
scaffolds
for
tissue
engineering
due
to
their
biocompatibility,
biodegradability
water
solubility.
Especially,
stiffness
hydrogels
is
key
factor,
which
influence
morphology
mesenchymal
stem
cells
(MSCs)
differentiation.
The
researches
on
this
point
are
meaningful
field
engineering.
Herein,
review
focus
design
with
different
effects
behavior
MSCs.
In
addition,
effect
hydrogel
phenotype
macrophages
introduced,
then
relationship
between
changes
inflammatory
response
discussed.
Finally,
future
application
certain
medicine
prospected.
International Journal of Molecular Sciences,
Journal Year:
2021,
Volume and Issue:
22(20), P. 11223 - 11223
Published: Oct. 18, 2021
Photobiomodulation
(PBM)
imparts
therapeutically
significant
benefits
in
the
healing
of
chronic
wounds.
Chronic
wounds
develop
when
stages
wound
fail
to
progress
a
timely
and
orderly
frame,
without
an
established
functional
structural
outcome.
Therapeutic
associated
with
PBM
include
augmenting
tissue
regeneration
repair,
mitigating
inflammation,
relieving
pain,
reducing
oxidative
stress.
stimulates
mitochondria,
resulting
increase
adenosine
triphosphate
(ATP)
production
downstream
release
growth
factors.
The
binding
factors
cell
surface
receptors
induces
signalling
pathways
that
transmit
signals
nucleus
for
transcription
genes
increased
cellular
proliferation,
viability,
migration
numerous
types,
including
stem
cells
fibroblasts.
Over
past
few
years,
advances
have
been
made
understanding
how
regulates
implicated
repair.
This
review
highlights
role
activation
several
involved
healing.
ACS Biomaterials Science & Engineering,
Journal Year:
2022,
Volume and Issue:
8(6), P. 2196 - 2219
Published: June 1, 2022
Designing
biomaterials
capable
of
biomimicking
wound
healing
and
skin
regeneration
has
been
receiving
increasing
attention
recently.
Some
biopolymers
behave
similarly
to
the
extracellular
matrix
(ECM),
supporting
biointerfacial
adhesion
intrinsic
cellular
interactions.
Polydopamine
(PDA)
is
a
natural
bioadhesive
bioactive
polymer
that
endows
high
chemical
versatility,
making
it
an
exciting
candidate
for
wide
range
biomedical
applications.
Moreover,
based
on
PDA
its
derivatives
have
near-infrared
(NIR)
absorption,
excellent
biocompatibility,
antioxidative
activity,
antibacterial
cell
affinity.
can
regulate
behavior
by
controlling
signal
transduction
pathways.
It
governs
focal
cells
at
interface.
These
features
make
melanin-like
fascinating
biomaterial
regeneration.
This
paper
overviews
PDA-based
biomaterials'
synthesis,
properties,
interactions
with
biological
entities.
Furthermore,
utilization
nano-
microstructures
as
constituent
wound-dressing
formulations
highlighted.
PLoS Biology,
Journal Year:
2023,
Volume and Issue:
21(5), P. e3001665 - e3001665
Published: May 30, 2023
Epithelial
repair
relies
on
the
activation
of
stress
signaling
pathways
to
coordinate
tissue
repair.
Their
deregulation
is
implicated
in
chronic
wound
and
cancer
pathologies.
Using
TNF-α/Eiger-mediated
inflammatory
damage
Drosophila
imaginal
discs,
we
investigate
how
spatial
patterns
behaviors
arise.
We
find
that
Eiger
expression,
which
drives
JNK/AP-1
signaling,
transiently
arrests
proliferation
cells
center
associated
with
a
senescence
program.
This
includes
production
mitogenic
ligands
Upd
family,
allows
JNK/AP-1-signaling
act
as
paracrine
organizers
regeneration.
Surprisingly,
cell-autonomously
suppress
via
Ptp61F
Socs36E,
both
negative
regulators
JAK/STAT
signaling.
As
suppressed
at
damage,
compensatory
occurs
by
periphery.
Mathematical
modelling
suggests
cell-autonomous
mutual
repression
between
core
regulatory
network
essential
spatially
separate
into
bistable
domains
distinct
cellular
tasks.
Such
stratification
for
proper
repair,
coactivation
same
creates
conflicting
signals
cell
cycle
progression,
leading
excess
apoptosis
senescently
stalled
organize
field.
Finally,
demonstrate
separation
senescent
proliferative
not
only
upon
but
also
Ras
V12
,
scrib
tumors.
Revealing
this
previously
uncharacterized
JNK/AP-1,
JAK/STAT,
has
important
implications
our
conceptual
understanding
pathologies,
tumor
microenvironments.
Journal of Cell Science,
Journal Year:
2024,
Volume and Issue:
137(20)
Published: May 13, 2024
Advances
in
imaging,
segmentation
and
tracking
have
led
to
the
routine
generation
of
large
complex
microscopy
datasets.
New
tools
are
required
process
this
'phenomics'
type
data.
Here,
we
present
'Cell
PLasticity
Analysis
Tool'
(cellPLATO),
a
Python-based
analysis
software
designed
for
measurement
classification
cell
behaviours
based
on
clustering
features
morphology
motility.
Used
after
tracking,
tool
extracts
from
each
per
timepoint,
using
them
segregate
cells
into
dimensionally
reduced
behavioural
subtypes.
Resultant
tracks
describe
'behavioural
ID'
at
similarity
allows
grouping
sequences
discrete
trajectories
with
assigned
IDs.
use
cellPLATO
investigate
role
IL-15
modulating
human
natural
killer
(NK)
migration
ICAM-1
or
VCAM-1.
We
find
eight
subsets
NK
their
shape
dynamics
between
single
timepoints,
four
these
over
time.
Therefore,
by
cellPLATO,
show
that
increases
plasticity
different
integrin
ligands
induce
forms
migration.
Journal of Biomedical Materials Research Part B Applied Biomaterials,
Journal Year:
2025,
Volume and Issue:
113(3)
Published: Feb. 20, 2025
ABSTRACT
Alginate
dressings
are
widely
used
in
wound
treatment
for
their
healing
and
hemostatic
properties
capacity
to
drain
exudate.
However,
a
clear
understanding
of
the
heterogeneity
within
this
class
is
lacking.
Numerous
sources
variability
exist
between
alginate
dressings:
composition
(%
calcium
relative
other
components),
ratio
D‐Mannuronic
L‐Guluronic
acids
fraction,
purity
(presence
toxic
contaminants),
shape
fibers
(surface
thickness).
These
parameters
affect
performance
safety
dressings,
which
may
thus
not
be
interchangeable
clinical
practice.
Therefore,
clinicians
must
aware
these
differences
ensure
optimal
avoid
complications
or
suboptimal
healing.
The
objective
study
was
compare
six
conclude
on
equivalence.
results
obtained
demonstrate
considerable
assessed
characteristics:
composition,
Ca
2+
release,
level
cytotoxicity,
fiber
shape,
draining
capacity,
resistance
traction.
Algostéril,
only
pure
rich
G,
releases
specific
dose
non‐cytotoxic
dressing.
With
its
multilobed
that
statistically
thickest,
it
provides
best
greatest
equivalent.
Each
dressing
distinct,
consequently
one
cannot
transposed
others.
each
should
own
efficacy,
given
indication,
through
trial.
Materials,
Journal Year:
2020,
Volume and Issue:
13(12), P. 2849 - 2849
Published: June 25, 2020
Bacterial
cellulose
(BC)
is
a
promising
material
for
biomedical
applications
due
to
its
unique
properties
such
as
high
mechanical
strength
and
biocompatibility.
This
article
describes
the
microbiological
synthesis,
modification,
characterization
of
obtained
BC-nanocomposites
originating
from
symbiotic
consortium
Medusomyces
gisevii.
Two
BC-modifications
have
been
obtained:
BC-Ag
BC-calcium
phosphate
(BC-Ca3(PO4)2).
Structure
physicochemical
BC
modifications
were
investigated
by
scanning
electron
microscopy
(SEM),
energy-dispersive
X-ray
spectroscopy
(EDX),
atomic
force
(AFM),
infrared
Fourier
well
measurements
water
holding/absorbing
capacities.
Topographic
analysis
surface
revealed
multicomponent
thick
fibrils
(150-160
nm
in
diameter
about
15
µm
length)
constituted
50-60
nanofibrils
weaved
into
left-hand
helix.
Distinctive
features
Ca-phosphate-modified
samples
(a)
presence
500-700
entanglements
(b)
inclusions
Ca3(PO4)2
crystals.
The
impregnated
with
Ag
nanoparticles
exhibited
numerous
roundish
inclusions,
110
diameter.
boundaries
between
organic
inorganic
phases
very
distinct
both
cases.
Ag-modified
also
showed
prominent
waving
pattern
packing
nanofibrils.
gel
films
possessed
water-holding
capacity
62.35
g/g.
However,
dried
(to
constant
mass)
BC-films
later
low
absorption
(3.82
g/g).
It
was
found
that
decellularized
had
2.4
times
larger
Young's
modulus
2.2
greater
tensile
compared
dehydrated
native
films.
We
presume
this
caused
molecular
compaction
structure.
WIREs Systems Biology and Medicine,
Journal Year:
2020,
Volume and Issue:
12(3)
Published: Feb. 8, 2020
Abstract
Tissue
repair
is
a
complex
process
that
requires
effective
communication
and
coordination
between
cells
across
multiple
tissues
organ
systems.
Two
of
the
initial
intracellular
signals
encode
injury
initiate
tissue
responses
are
calcium
extracellular
signal‐regulated
kinase
(ERK).
However,
ERK
signaling
control
variety
cellular
behaviors
important
for
including
motility,
contractility,
proliferation,
as
well
activity
several
different
transcription
factors,
making
it
challenging
to
relate
specific
their
respective
programs.
This
knowledge
gap
ultimately
hinders
development
new
wound
healing
therapies
could
take
advantage
native
programs
more
effectively
damage.
The
objective
this
review
highlight
roles
dynamics
mechanisms
link
during
epithelial
stromal
repair.
We
detail
how
networks
controlling
can
now
also
be
dissected
using
classical
signal
processing
techniques
with
advent
biosensors
optogenetic
controllers.
Finally,
we
advocate
importance
recognizing
key
links
detection
both
organize
execute
coordinated
response
organs.
article
categorized
under:
Models
Systems
Properties
Processes
>
Mechanistic
Biological
Mechanisms
Cell
Signaling
Laboratory
Methods
Technologies
Imaging
Organ,
Tissue,
Physiological
Bioactive Materials,
Journal Year:
2022,
Volume and Issue:
18, P. 368 - 382
Published: Feb. 12, 2022
To
control
capillary
bleeding,
surgeons
may
use
absorbable
hemostatic
agents,
such
as
Surgicel®
and
TachoSil®.
Due
to
their
slow
resorption,
persistence
in
situ
can
have
a
negative
impact
on
tissue
repair
the
resected
organ.
avoid
complications
obtain
agent
that
promotes
repair,
zinc-supplemented
calcium
alginate
compress
was
developed:
HEMO-IONIC®.
This
is
non-absorbable
therefore
removed
once
hemostasis
has
been
achieved.
After
demonstrating
efficacy
stability
of
blood
clot
obtained
with
HEMO-IONIC,
Surgicel,
TachoSil,
HEMO-IONIC
cell
activation
were
compared
(i)
vitro
endothelial
cells,
which
are
essential
(ii)
vivo
mouse
skin
excision
model.
In
vitro,
only
maintained
phenotypic
functional
properties
cells
induced
migration.
comparison,
Surgicel
found
be
highly
cytotoxic,
TachoSil
inhibited
vivo,
increased
angiogenesis,
recruitment
(macrophages,
fibroblasts,
epithelial
cells),
accelerated
maturation
extracellular
matrix.
These
results
demonstrate
alginate,
applied
for
10
min
at
end
surgery
then
long-term
positive
effect
all
phases
repair.