A Review on the Design of Hydrogels With Different Stiffness and Their Effects on Tissue Repair

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: Jan. 25, 2022

Tissue repair after trauma and infection has always been a difficult problem in regenerative medicine. Hydrogels have become one of the most important scaffolds for tissue engineering due to their biocompatibility, biodegradability water solubility. Especially, stiffness hydrogels is key factor, which influence morphology mesenchymal stem cells (MSCs) differentiation. The researches on this point are meaningful field engineering. Herein, review focus design with different effects behavior MSCs. In addition, effect hydrogel phenotype macrophages introduced, then relationship between changes inflammatory response discussed. Finally, future application certain medicine prospected.

Language: Английский

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Cellular Signalling and Photobiomodulation in Chronic Wound Repair

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(20), P. 11223 - 11223

Published: Oct. 18, 2021

Photobiomodulation (PBM) imparts therapeutically significant benefits in the healing of chronic wounds. Chronic wounds develop when stages wound fail to progress a timely and orderly frame, without an established functional structural outcome. Therapeutic associated with PBM include augmenting tissue regeneration repair, mitigating inflammation, relieving pain, reducing oxidative stress. stimulates mitochondria, resulting increase adenosine triphosphate (ATP) production downstream release growth factors. The binding factors cell surface receptors induces signalling pathways that transmit signals nucleus for transcription genes increased cellular proliferation, viability, migration numerous types, including stem cells fibroblasts. Over past few years, advances have been made understanding how regulates implicated repair. This review highlights role activation several involved healing.

Language: Английский

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Polydopamine Biomaterials for Skin Regeneration

ACS Biomaterials Science & Engineering, Journal Year: 2022, Volume and Issue: 8(6), P. 2196 - 2219

Published: June 1, 2022

Designing biomaterials capable of biomimicking wound healing and skin regeneration has been receiving increasing attention recently. Some biopolymers behave similarly to the extracellular matrix (ECM), supporting biointerfacial adhesion intrinsic cellular interactions. Polydopamine (PDA) is a natural bioadhesive bioactive polymer that endows high chemical versatility, making it an exciting candidate for wide range biomedical applications. Moreover, based on PDA its derivatives have near-infrared (NIR) absorption, excellent biocompatibility, antioxidative activity, antibacterial cell affinity. can regulate behavior by controlling signal transduction pathways. It governs focal cells at interface. These features make melanin-like fascinating biomaterial regeneration. This paper overviews PDA-based biomaterials' synthesis, properties, interactions with biological entities. Furthermore, utilization nano- microstructures as constituent wound-dressing formulations highlighted.

Language: Английский

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Mutual repression between JNK/AP-1 and JAK/STAT stratifies senescent and proliferative cell behaviors during tissue regeneration

PLoS Biology, Journal Year: 2023, Volume and Issue: 21(5), P. e3001665 - e3001665

Published: May 30, 2023

Epithelial repair relies on the activation of stress signaling pathways to coordinate tissue repair. Their deregulation is implicated in chronic wound and cancer pathologies. Using TNF-α/Eiger-mediated inflammatory damage Drosophila imaginal discs, we investigate how spatial patterns behaviors arise. We find that Eiger expression, which drives JNK/AP-1 signaling, transiently arrests proliferation cells center associated with a senescence program. This includes production mitogenic ligands Upd family, allows JNK/AP-1-signaling act as paracrine organizers regeneration. Surprisingly, cell-autonomously suppress via Ptp61F Socs36E, both negative regulators JAK/STAT signaling. As suppressed at damage, compensatory occurs by periphery. Mathematical modelling suggests cell-autonomous mutual repression between core regulatory network essential spatially separate into bistable domains distinct cellular tasks. Such stratification for proper repair, coactivation same creates conflicting signals cell cycle progression, leading excess apoptosis senescently stalled organize field. Finally, demonstrate separation senescent proliferative not only upon but also Ras V12 , scrib tumors. Revealing this previously uncharacterized JNK/AP-1, JAK/STAT, has important implications our conceptual understanding pathologies, tumor microenvironments.

Language: Английский

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cellPLATO – an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(20)

Published: May 13, 2024

Advances in imaging, segmentation and tracking have led to the routine generation of large complex microscopy datasets. New tools are required process this 'phenomics' type data. Here, we present 'Cell PLasticity Analysis Tool' (cellPLATO), a Python-based analysis software designed for measurement classification cell behaviours based on clustering features morphology motility. Used after tracking, tool extracts from each per timepoint, using them segregate cells into dimensionally reduced behavioural subtypes. Resultant tracks describe 'behavioural ID' at similarity allows grouping sequences discrete trajectories with assigned IDs. use cellPLATO investigate role IL-15 modulating human natural killer (NK) migration ICAM-1 or VCAM-1. We find eight subsets NK their shape dynamics between single timepoints, four these over time. Therefore, by cellPLATO, show that increases plasticity different integrin ligands induce forms migration.

Language: Английский

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Are All Alginate Dressings Equivalent?

Journal of Biomedical Materials Research Part B Applied Biomaterials, Journal Year: 2025, Volume and Issue: 113(3)

Published: Feb. 20, 2025

ABSTRACT Alginate dressings are widely used in wound treatment for their healing and hemostatic properties capacity to drain exudate. However, a clear understanding of the heterogeneity within this class is lacking. Numerous sources variability exist between alginate dressings: composition (% calcium relative other components), ratio D‐Mannuronic L‐Guluronic acids fraction, purity (presence toxic contaminants), shape fibers (surface thickness). These parameters affect performance safety dressings, which may thus not be interchangeable clinical practice. Therefore, clinicians must aware these differences ensure optimal avoid complications or suboptimal healing. The objective study was compare six conclude on equivalence. results obtained demonstrate considerable assessed characteristics: composition, Ca 2+ release, level cytotoxicity, fiber shape, draining capacity, resistance traction. Algostéril, only pure rich G, releases specific dose non‐cytotoxic dressing. With its multilobed that statistically thickest, it provides best greatest equivalent. Each dressing distinct, consequently one cannot transposed others. each should own efficacy, given indication, through trial.

Language: Английский

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Bacterial Cellulose Nanocomposites: Morphology and Mechanical Properties

Materials, Journal Year: 2020, Volume and Issue: 13(12), P. 2849 - 2849

Published: June 25, 2020

Bacterial cellulose (BC) is a promising material for biomedical applications due to its unique properties such as high mechanical strength and biocompatibility. This article describes the microbiological synthesis, modification, characterization of obtained BC-nanocomposites originating from symbiotic consortium Medusomyces gisevii. Two BC-modifications have been obtained: BC-Ag BC-calcium phosphate (BC-Ca3(PO4)2). Structure physicochemical BC modifications were investigated by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic force (AFM), infrared Fourier well measurements water holding/absorbing capacities. Topographic analysis surface revealed multicomponent thick fibrils (150-160 nm in diameter about 15 µm length) constituted 50-60 nanofibrils weaved into left-hand helix. Distinctive features Ca-phosphate-modified samples (a) presence 500-700 entanglements (b) inclusions Ca3(PO4)2 crystals. The impregnated with Ag nanoparticles exhibited numerous roundish inclusions, 110 diameter. boundaries between organic inorganic phases very distinct both cases. Ag-modified also showed prominent waving pattern packing nanofibrils. gel films possessed water-holding capacity 62.35 g/g. However, dried (to constant mass) BC-films later low absorption (3.82 g/g). It was found that decellularized had 2.4 times larger Young's modulus 2.2 greater tensile compared dehydrated native films. We presume this caused molecular compaction structure.

Language: Английский

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Intracellular signaling dynamics and their role in coordinating tissue repair

WIREs Systems Biology and Medicine, Journal Year: 2020, Volume and Issue: 12(3)

Published: Feb. 8, 2020

Abstract Tissue repair is a complex process that requires effective communication and coordination between cells across multiple tissues organ systems. Two of the initial intracellular signals encode injury initiate tissue responses are calcium extracellular signal‐regulated kinase (ERK). However, ERK signaling control variety cellular behaviors important for including motility, contractility, proliferation, as well activity several different transcription factors, making it challenging to relate specific their respective programs. This knowledge gap ultimately hinders development new wound healing therapies could take advantage native programs more effectively damage. The objective this review highlight roles dynamics mechanisms link during epithelial stromal repair. We detail how networks controlling can now also be dissected using classical signal processing techniques with advent biosensors optogenetic controllers. Finally, we advocate importance recognizing key links detection both organize execute coordinated response organs. article categorized under: Models Systems Properties Processes > Mechanistic Biological Mechanisms Cell Signaling Laboratory Methods Technologies Imaging Organ, Tissue, Physiological

Language: Английский

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A new hemostatic agent composed of Zn2+-enriched Ca2+ alginate activates vascular endothelial cells in vitro and promotes tissue repair in vivo

Bioactive Materials, Journal Year: 2022, Volume and Issue: 18, P. 368 - 382

Published: Feb. 12, 2022

To control capillary bleeding, surgeons may use absorbable hemostatic agents, such as Surgicel® and TachoSil®. Due to their slow resorption, persistence in situ can have a negative impact on tissue repair the resected organ. avoid complications obtain agent that promotes repair, zinc-supplemented calcium alginate compress was developed: HEMO-IONIC®. This is non-absorbable therefore removed once hemostasis has been achieved. After demonstrating efficacy stability of blood clot obtained with HEMO-IONIC, Surgicel, TachoSil, HEMO-IONIC cell activation were compared (i) vitro endothelial cells, which are essential (ii) vivo mouse skin excision model. In vitro, only maintained phenotypic functional properties cells induced migration. comparison, Surgicel found be highly cytotoxic, TachoSil inhibited vivo, increased angiogenesis, recruitment (macrophages, fibroblasts, epithelial cells), accelerated maturation extracellular matrix. These results demonstrate alginate, applied for 10 min at end surgery then long-term positive effect all phases repair.

Language: Английский

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Exosome Mediated Cell-Cell Crosstalk in Tissue Injury and Repair

Results and problems in cell differentiation, Journal Year: 2024, Volume and Issue: unknown, P. 249 - 297

Published: Jan. 1, 2024

Language: Английский

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