Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools—Chances and Limitations: A Critical Review DOI Open Access
Joachim Müller, Ghalia Boubaker,

Norbert Müller

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6903 - 6903

Published: June 24, 2024

Identification of drug targets and biochemical investigations on mechanisms action are major issues in modern development. The present article is a critical review the classical "one drug"-"one target" paradigm. In fact, novel methods for target deconvolution investigation resistant strains based protein mass spectrometry have shown that multiple gene products adaptation involved responses pathogens to xenobiotics rather than one single or product. Resistance drugs may be linked differential expression other proteins those interacting with binding studies result complex cell physiological adaptation. Consequently, unraveling needs approaches beyond proteomics. This focused protozoan pathogens. conclusions can, however, extended chemotherapies against cancer.

Language: Английский

Fluorescence-Based Protein Stability Monitoring—A Review DOI Open Access

Negin Gooran,

Kari Kopra

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1764 - 1764

Published: Feb. 1, 2024

Proteins are large biomolecules with a specific structure that is composed of one or more long amino acid chains. Correct protein structures directly linked to their correct function, and many environmental factors can have either positive negative effects on this structure. Thus, there clear need for methods enabling the study proteins, folding, components affecting stability. There significant number label-free In review, we provide general overview these methods, but main focus fluorescence-based low-instrument -expertise-demand techniques. Different aspects related thermal shift assays (TSAs), also called differential scanning fluorimetry (DSF) ThermoFluor, introduced compared isothermal chemical denaturation (ICD). Finally, discuss challenges comparative as well future opportunities assay development directions.

Language: Английский

Citations

14
Recent advances in high-throughput screening methods for small molecule modulators in bacteria DOI

Hannah G Addis,

Erin E. Carlson

Current Opinion in Chemical Biology, Journal Year: 2025, Volume and Issue: 85, P. 102571 - 102571

Published: Feb. 14, 2025

Language: Английский

Citations

0
Study on the Mechanism of Action of the Pt(IV) Complex with Lonidamine Ligands by Ultrafast Chemical Proteomics DOI Creative Commons

Ekaterina A.. Imaikina,

Ivan I. Fedorov, Daria D. Emekeeva

et al.

ACS Pharmacology & Translational Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Platinum(II) complexes such as cisplatin, among a few others, are well-known anticancer metal-based drugs approved for clinical use. In spite of their wide acceptance, the respective chemotherapy is associated with severe side effects and ability tumors to quickly develop resistance. To overcome these drawbacks, novel strategy considered, which based on use platinum bioactive ligands attached act in synergy further improve its pharmacological properties. Among recently introduced multiaction prodrugs Pt(IV) complex two lonidamine ligands, latter selectively inhibiting hexokinase and, thus, glycolysis cancer cells. While platinum-based exhibit increased levels activity toward cells considered potent resistance there crucial need uncover mechanism action by revealing all possibly affected processes targets across whole cellular proteome. These challenging tasks proteomics requiring high-throughput analysis hundreds samples just single drug-to-proteome system. this work, we performed analyses 8-azaguanine experimental Pt(IV)-lonidamine applied ovarian cell line A2780 employing both mechanism- compound-centric ultrafast chemical approaches. approaches were protein expression thermal proteome profiling, respectively. Data obtained revealed regulation proteins involved glucose metabolic process lonidamine, supporting prodrug action.

Language: Английский

Citations

0
On the utility of ultrafast MS1-only proteomics in drug target discovery studies based on thermal proteome profiling method DOI
Ivan I. Fedorov, Julia A. Bubis, Elizaveta M. Kazakova

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: 416(18), P. 4083 - 4089

Published: May 15, 2024

Language: Английский

Citations

2
Nanoparticle-Based Delivery Platforms for the Enhanced Oral Delivery of Peptides/Proteins DOI
Sara Salatin, Soheila Montazersaheb, Afsaneh Farjami

et al.

Therapeutic Delivery, Journal Year: 2023, Volume and Issue: 14(12), P. 795 - 815

Published: Dec. 1, 2023

Biopharmaceutical products are currently well-established in nearly all branches of medicine and believed to have great potential for the treatment a broad spectrum diseases. Peptide/protein drugs exhibit predominant class new biopharmaceuticals coming on market recent years. Oral delivery peptides/proteins as non-invasive therapeutic technique has become an appealing alternative parenteral route. However, efficient oral is limited because their high molecular weight, poor stability low biodistribution. Nanoparticles (NPs) shown excellent results peptide/protein research. In this paper, use NPs systems ability be efficiently delivered via route been described.

Language: Английский

Citations

1
Investigating Antiprotozoal Chemotherapies with Novel Proteomic Tools—Chances and Limitations: A Critical Review DOI Open Access
Joachim Müller, Ghalia Boubaker,

Norbert Müller

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6903 - 6903

Published: June 24, 2024

Identification of drug targets and biochemical investigations on mechanisms action are major issues in modern development. The present article is a critical review the classical "one drug"-"one target" paradigm. In fact, novel methods for target deconvolution investigation resistant strains based protein mass spectrometry have shown that multiple gene products adaptation involved responses pathogens to xenobiotics rather than one single or product. Resistance drugs may be linked differential expression other proteins those interacting with binding studies result complex cell physiological adaptation. Consequently, unraveling needs approaches beyond proteomics. This focused protozoan pathogens. conclusions can, however, extended chemotherapies against cancer.

Language: Английский

Citations

0