A multifaceted approach to investigate interactions of thifluzamide with haemoglobin DOI
Sandeep Yadav, Shubham Sewariya,

Anirudh Pratap Singh Raman

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136736 - 136736

Published: Oct. 1, 2024

Language: Английский

Enzymatic Cascade Transformation of Renewable C1 and C2 Alcohols into 3-Hydroxypropionaldehyde with a High Conversion Rate DOI
Min‐Ju Seo, Yeon‐Ju Jeong,

Su-Bin Ju

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Language: Английский

Citations

2
Differential scanning fluorimetry to assess PFAS binding to bovine serum albumin protein DOI Creative Commons
Jessica Alesio, Geoffrey D. Bothun

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 18, 2024

Abstract The rapid screening of protein binding affinity for poly- and perfluoroalkyl substances (PFAS) benefits risk assessment fate transport modelling. PFAS are known to bioaccumulate in livestock through contaminated food water. One excretion pathway is milk, which may be facilitated by milk proteins such as bovine serum albumin (BSA). We report a label-free differential scanning fluorimetry approach determine PFAS–BSA over broad temperature range. This method utilizes the tryptophan residue within pocket an intrinsic fluorophore, eliminating need fluorophore labels that influence binding. BSA association constants were determined (a) equilibrium-based model at melting (b) Hill adsorption account dependent cooperativity. Differences between fatty acid analogs revealed combination size hydrophobicity drives

Language: Английский

Citations

4
A structural and biochemical approach to effect of temperature and pH on the Molten Globule phenomenon in a thermophilic protease: Molecular dynamics simulation, fluorescence spectroscopy and circular dichroism DOI

Ghadir A. Jamal,

Ehsan Jahangirian, Michael R. Hamblin

et al.

Journal of Molecular Structure, Journal Year: 2024, Volume and Issue: 1318, P. 139204 - 139204

Published: July 6, 2024

Language: Английский

Citations

4
Rapid Development of High Concentration Protein Formulation Driven by High-Throughput Technologies DOI Creative Commons

Lun Xin,

Monika Prorok,

Zhe Zhang

et al.

Pharmaceutical Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Language: Английский

Citations

0
High-throughput virtual screening and empirical validation of probable inhibitors of Plasmodium falciparum and vivax glutathione transferase using bromosulfophthalein as the benchmark ligand DOI
Olalekan Onisuru, Ikechukwu Achilonu

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140526 - 140526

Published: Jan. 1, 2025

Language: Английский

Citations

0
Recent Developments in 14–3–3 Stabilizers for Regulating Protein–Protein Interactions: An Update DOI
Yucheng Tian, Lan Li,

Liuyi Wu

et al.

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

14–3–3 proteins play a crucial role in the regulation of protein–protein interactions, impacting various cellular processes and disease mechanisms. Recent advancements have led to development stabilizers that enhance binding clients, presenting promising therapeutic potentials. This perspective provides an updated overview latest developments field stabilizers, with focus on their design, synthesis, biological evaluation. We discuss structural basis for interaction between ligands, highlighting key modifications affinity selectivity. Additionally, we explore applications across major areas such as cancer, metabolic disorders, neurodegenerative diseases. By summarizing recent research findings technological advancements, this aims shed light current state stabilizer outline future directions optimizing these compounds effective agents.

Language: Английский

Citations

0
Design, Synthesis, and Application of Triazole-Embedded Maltose-Neopentyl-Glycol (Tmng) Amphiphiles for Membrane Protein Studies DOI

Hongliang Cai,

Xinyu Wu, Shixin Jin

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cholinium-Based Ionic Liquids Modulate Protein Stability: A Comparative Study of Enzymes and Albumins DOI Creative Commons
Artashes A. Khachatrian, Timur A. Mukhametzyanov, Ramazan Z. Salikhov

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1574 - 1574

Published: March 31, 2025

This work aims to assess the intermolecular interaction of choline ionic liquids (ILs) (choline malonate ([Ch][Mal]), succinate ([Ch][Suc]), and valinate ([Ch][Val]) with two enzymes (lysozyme α-chymotrypsin). We evaluated state tertiary protein structure using circular dichroism (CD) spectrometry quantified binding parameters by fluorescence spectroscopy. The energies localization ions on them were estimated molecular docking. then analyzed relationship between enzymes’ thermostability their tendency towards aggregation in enzyme/ionic liquid systems. obtained results compared previous data albumins identify similarities differences behavior solutions. Despite comparable values constants, effect was opposite albumins. In addition, although these promoted both albumins, this much more pronounced for

Language: Английский

Citations

0
Proteomics in decoding cancer: A review DOI

Elaheh Gheybi,

Parisa Hosseinzadeh,

Vahid Tayebi-Khorrami

et al.

Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120302 - 120302

Published: April 1, 2025

Language: Английский

Citations

0
Functional and structural characterization of the human indolethylamine N-methyltransferase through fluorometric, thermal and computational docking analyses DOI Creative Commons
Matteo Ardini, Francesco Angelucci,

Francesca Rea

et al.

Biology Direct, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 10, 2025

The "psychedelic renaissance" is sparking growing interest in clinical research, along with a rise trials. Substances such as 3,4-methylenedioxymethamphetamine (MDMA), psilocybin and N,N-dimethyltryptamine (DMT) are involved. focus of this paper on indolethylamine N-methyltransferase (INMT), crucial enzyme the biosynthesis key compounds, including DMT, which meets science, medicine spirituality. presence DMT animals plants raises many questions about its biological role. Meanwhile, distribution INMT various organs involvement diseases like cancer mental disorders also fuel investigations worldwide. However, remains largely unexplored, particularly enzymatic mechanism structural properties, leaving significant gap potential applications. This study examines for first time catalytic activity human (hINMT) using simple fluorometric steady-state assay employing substrate quinoline. findings supported by thermal shift docking analyses, providing valuable information optimal chemical conditions binding sites substrates. assays indicate that recombinant hINMT unstable requires acidic or near-neutral pH low salt levels. These experiments allow estimation dissociation constants natural coenzymes SAM SAH, helping to determine appropriate setup calculate kinetic constants, comparable other methyltransferases. indicates quinoline occupies same site tryptamine, further validating approach. provides foundation thoroughly studying under consistent conditions, obtaining reliable data maintaining molecular stability future analysis. represents valid alternative over previous endpoint radioactive-based chromatography-mass spectrometry assays, can provide only apparent parameters. Given polymorphisms observed their association psychiatric disorders, e.g., schizophrenia, cancer, strategy could serve an invaluable tool understanding structure-function relationship mutants role diseases. Furthermore, these insights into interaction modalities substrates lay groundwork inhibition aimed at practical

Language: Английский

Citations

0