Hindering the biofilm of microbial pathogens and cancer cell lines development using silver nanoparticles synthesized by epidermal mucus proteins from Clarias gariepinus

BMC Biotechnology, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 4, 2024

Abstract Scientists know very little about the mechanisms underlying fish skin mucus, despite fact that it is a component of immune system. Fish mucus an important defence against invasive infections. Recently, and its are gaining interest among immunologists. Characterization was done on obtained silver nanoparticles Ag combined with Clarias gariepinus catfish epidermal proteins (EMP-Ag-NPs) through UV–vis, FTIR, XRD, TEM, SEM. Ag-NPs ranged in size from 4 to 20 nm, spherical form angles were 38.10°, 44.20°, 64.40°, 77.20°, Where wavelength change after formation EMP-Ag-NPs as indicate dark brown, broad band recorded at 391 nm. Additionally, antimicrobial, antibiofilm anticancer activities assessed. The present results demonstrate high activity unicellular fungi C. albicans , followed by E. faecalis . Antibiofilm showed strong both S. aureus P. aeruginosa pathogens dose-dependent manner, without affecting planktonic cell growth. Also, cytotoxicity effect investigated normal cells (Vero), breast cancer (Mcf7) hepatic carcinoma (HepG2) lines concentrations (200–6.25 µg/mL) current highly 100, 5 25 µg/mL exhibited rounding, shrinkage, deformation granulation Mcf7 HepG2 IC50 19.34 31.16 respectively while Vero appeared rounded concentration 50 shape 25, 12.5 6.25 µg/ml 35.85 µg/mL. This study evidence potential efficacy biologically generated substitute medicinal agent harmful microorganisms. Furthermore, highlights their inhibitory lines.

Language: Английский

---

Biosynthesis and characterization of selenium and silver nanoparticles using Trichoderma viride filtrate and their impact on Culex pipiens

Green Processing and Synthesis, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Some of the significant globally prevalent vector-borne illnesses are caused by Culex pipiens . Synthetic pesticides have been widely utilized to eradicate C. , which has led a number health risks for people, insect resistance, and environmental contamination. Alternative strategies therefore vitally needed. In current investigation, Trichoderma viride fungal culture filtrate was used create selenium silver nanoparticles (SeNPs AgNPs, respectively) test them on larvae in their fourth instar stage. The death rate increased significantly when SeNP AgNP concentrations increased, according results. SeNPs AgNPs affected developmental detoxification enzymes at 24 h after being treated with sublethal concentration tested NPs. As result insecticidal effect larvae, considered effective promising larvicidal agents.

Language: Английский

---

Optimization Growth Conditions of Fusarium pseudonygamai for Myco-Synthesized Gold and Silver Nanoparticles Using Response Surface Methodology

BioNanoScience, Journal Year: 2024, Volume and Issue: 14(5), P. 5420 - 5435

Published: March 9, 2024

Language: Английский

---

Nanotechnology for Healthcare: Plant-Derived Nanoparticles in Disease Treatment and Regenerative Medicine

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(12), P. 1711 - 1711

Published: Dec. 18, 2024

Nanotechnology has revolutionised biomedical research, offering innovative healthcare solutions. Plant-based nanotechnology is emerging as a sustainable alternative, minimising environmental impacts and enhancing therapeutic effectiveness. This paper explores the potential of plant-derived nanoparticles (PNPs) in medicine, highlighting their biocompatibility, multifunctionality, eco-friendliness. PNPs, synthesised through green methods, have demonstrated promising applications drug delivery, cancer therapy, antimicrobial treatments, tissue regeneration. Their unique properties, such high surface area bioactive components, enable improved targeting, controlled release, reducing side effects treatment efficacy. Additionally, compounds’ inherent antioxidant retained within platinum (PNPs), present opportunities for combating resistance promoting wound healing. Despite potential, challenges remain standardising PNP synthesis, ensuring consistency, scaling up production industrial applications. review emphasises need further research on toxicity, regulatory frameworks to fully harness capabilities clinical commercial represents promising, greener alternative advancing solutions, aligning with global sustainability goals.

Language: Английский

---

Green synthesis of selenium nanoparticles from Cassia javanica flowers extract and their medical and agricultural applications

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 5, 2024

Abstract Nanostructured materials are advantageous within numerous fields of medicine owing to their intriguing qualities, which include size, reactive surface, bioactivity, potential for modification, and optical characteristics. Cassia javanica flower extract was used as a chelating agent in an environmentally friendly process create SeNPs FTIR, XRD, TEM, SAED were utilized analyze characterize the synthesized. The findings showed that MIC Se NPs against B. subtilis S. aureus 500 µg/ml. Conversely, P. aeruginosa , E. coli C. albicans 125, 250, 62.5 µg/ml, respectively. Hence, considerably reduced activity; inhibition peaked at 77.6% 250 µg/ml reach 49.04% 7.8 Which greatest suppression MRSA biofilm formation without affecting bacterial growth. antioxidant capacity, achieving IC 50 53.34 This study looked how soaking seeds before sowing them with 50, 100, 200 ppm affected plants’ development different parameters, well yield Vicia faba L. growth conditions effectively increased by application various quantities NPs. highest values dry weight/pod (g), number seeds/plant, weight 100 pods/plant caused high concentrations NPs, 28.43, 89.60, 18.20, 94.11%,

Language: Английский

---

Role of Carbon-Based Nanomaterials as a Bactericides and Fungicides in Agricultural System

Published: Jan. 1, 2025

Language: Английский

---

Biosynthesis of Silver Nanoparticles from Endophytic Fungi and Their Role in Plant Disease Management

Published: Jan. 1, 2025

Language: Английский

---

Exploring the Potential of Agro‐Nanotechnology in African Agriculture: A Path to Sustainable Development—Systematic Review

The Scientific World JOURNAL, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Agro-nanotechnology-the application of nanotechnology in agriculture-holds immense promise for addressing main challenges African agriculture and promoting sustainable development. This review provides a comprehensive analysis how agro-nanotechnology is being utilized across Africa, emphasizing its potential to revolutionize various aspects agricultural practices on the continent. Firstly, utilization nanomaterials such as nanoparticles, nanofertilizers, nanopesticides offers opportunities enhancing nutrient management, improving soil health, increasing crop productivity diverse agroecosystems Africa. Nanofertilizers, with their controlled release mechanisms, facilitate efficient uptake by plants, thereby reducing losses fertilizer use efficiency, which crucial resource-constrained smallholder farmers. Nanopesticides suggest improved efficacy pest disease control, environmental harm compared traditional pesticides. Their targeted delivery also minimizes off-target effects, Africa's food security. Nanosensors enable real-time monitoring precision agriculture. Nanotechnology postharvest management reduces improves safety. However, adoption requires careful consideration socioeconomic regulatory factors ensure equitable access Collaborative efforts involving policymakers, researchers, farmers, other stakeholders are harnessing benefits while risks concerns. In conclusion, integration into presents transformative opportunity enhance productivity, resilience, sustainability, contributing continent's toward achieving security, economic development, conservation.

Language: Английский

---

A comprehensive study on characterization of biosynthesized copper-oxide nanoparticles, their capabilities as anticancer and antibacterial agents, and predicting optimal docking poses into the cavity of S. aureus DHFR

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(4), P. e0319791 - e0319791

Published: April 1, 2025

The eco-friendly method of producing copperـoxide nanoparticles through the use okra fruit extract is a simple, economical, rapid, and sustainable technique. resultant (CuO NP) were analyzed with several analytical methods, such as UV-vis spectroscopy, FourierـTransform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Zeta potential, TransmissionـElectron Microscopy (TEM) EnergyـDispersive X-ray (EDX) analysis. CuO NP exhibited maximum absorbance at 381 nm. formation was further confirmed by characteristic bands observed 534 588 cm -1 . monoclinic structure identified prominent peaks detected 2θ values 32.47°, 35.43°, 38.64°, 48.68°, 53.38°, 58.14°, 61.39°, 66.11°, 67.82°, 72.27°, 74.96°. overall findings indicate that had an average diameter in approximate range 10 to 30 nm based on TEM cytotoxicity study, conducted Human Fibroblast normal HFB4 cell lines, indicated halfـmaximal inhibitory concentration (IC50) dose 236.34 μg/mL. An IC50 109.46 μg/mL found antitumor effect studies using breast adenocarcinoma Mcf- 7 revealing good level safety for NP. According antibacterial Staphylococcus aureus Bacillus cereus inhibition zone diameters (IZDs) 29.5 ± 0.7 mm 24.6 1.2 mm, respectively, making them most vulnerable bacteria In contrast, P. aeruginosa least sensitive strain, minimum IZD 15 1.6 mm. Compared gram-negative infections, NPs have significantly higher effectiveness versus Gram -positive pathogens. Molecular docking against dihydrofolate reductase (DHFR) (PDB ID: 6P9Z) illustrated partially interlocked active site 6P9Z fitting energy value -44.93 kcal/mol five classical hydrogen bonds Ala7, Gln9, Thr46, Ser49, Phe92. last one also generated marketing antifolate agent methotrexate (MTX), adding some MTX-like character inhibitor.

Language: Английский

---

Gold Nanoparticles as the Sterilant in Large-Scale Micropropagation of Plants

Published: Jan. 1, 2024

Language: Английский

---