Systematic Analysis of Long Non-Coding RNAs in Inflammasome Activation in Monocytes/Macrophages DOI Creative Commons

Qian Na,

Rebecca Distefano, Mirolyuba Ilieva

et al.

Non-Coding RNA, Journal Year: 2023, Volume and Issue: 9(5), P. 50 - 50

Published: Aug. 25, 2023

The NLRP3 inflammasome plays a pivotal role in regulating inflammation and immune responses. Its activation can lead to an inflammatory response pyroptotic cell death. This is beneficial the case of infections, but excessive chronic tissue damage. Moreover, while most mammalian genome transcribed as RNAs, only small fraction codes for proteins. Among non-protein-coding long non-coding RNAs (lncRNAs) have been shown play key roles gene expression cellular processes. They interact with DNA, proteins, their dysregulation provide insights into disease mechanisms, including activation. Here, we systematically analyzed previously published RNA sequencing (RNA-seq) data monocytes/macrophages uncover inflammasome-regulated lncRNA genes. To functional importance genes, one lncRNA, ENSG00000273124, was knocked down vitro model macrophage polarization. results indicate that silencing ENSG00000273124 resulted up-regulation tumor necrosis factor (TNF), suggesting this might be involved pro-inflammatory macrophages. make our more accessible, developed web database InflammasomeDB.

Language: Английский

NLRP3 Inflammasome Involvement in Heart, Liver, and Lung Diseases—A Lesson from Cytokine Storm Syndrome DOI Open Access
Cecilia Napodano,

Valeria Carnazzo,

Valerio Basile

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(23), P. 16556 - 16556

Published: Nov. 21, 2023

Inflammation and inflammasomes have been proposed as important regulators of the host-microorganism interaction, playing a key role in morbidity mortality due to coronavirus disease 2019 (COVID-19) subjects with chronic conditions compromised immune system. The inflammasome consists multiprotein complex that finely regulates activation caspase-1 production secretion potent pro-inflammatory cytokines such IL-1β IL-18. pyrin containing NOD (nucleotide-binding oligomerization domain) like receptor (NLRP) is family intracellular receptors, sensing patterns associated pathogens or danger signals NLRP3 most deeply analyzed for its involvement innate adaptive system well contribution several autoinflammatory autoimmune diseases. It highly expressed leukocytes up-regulated sentinel cells upon inflammatory stimuli. expression has also reported B T lymphocytes, epithelial oral genital mucosa, specific parenchymal cardiomyocytes, keratinocytes, chondrocytes. known dysregulated involved pathogenesis different disorders share common red line inflammation their pathogenetic fingerprint. Here, we review potential roles cardiovascular events, liver damage, pulmonary diseases, wide range systemic syndromes named cytokine storm.

Language: Английский

Citations

16
New insights into crosstalk between Nrf2 pathway and ferroptosis in lung disease DOI Creative Commons

Yonghu Chen,

Zhe Jiang, Xuezheng Li

et al.

Cell Death and Disease, Journal Year: 2024, Volume and Issue: 15(11)

Published: Nov. 18, 2024

Abstract Ferroptosis is a distinctive process of cellular demise that linked to amino acid metabolism, lipid oxidation, and iron oxidation. The ferroptosis cascade genes, which are closely associated with the onset lung diseases, among regulatory targets nuclear factor erythroid 2-related 2 (Nrf2). Although regulation mostly mediated by Nrf2, precise roles underlying mechanisms Nrf2 in illness remain unclear. This review provides new insights from recent discoveries involving modulation range diseases. It also systematically describes peroxidation, intracellular antioxidant levels, ubiquitination expression FSP1 GPX4. Finally, it summarises active ingredients drugs potential for treatment With overarching aim expediting improvements treatment, this reference novel therapeutic offers suggestions development medications variety disorders.

Language: Английский

Citations

5
KAE ameliorates LPS-mediated acute lung injury by inhibiting PANoptosis through the intracellular DNA-cGAS-STING axis DOI Creative Commons

Yonghu Chen,

Xilin Wu, Zhe Jiang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Acute lung injury (ALI) is a severe condition characterized by inflammation, tissue damage, and persistent activation of the cyclic GMP-AMP (cGAS)-stimulator interferon genes (STING) pathway, which exacerbates production pro-inflammatory mediators promotes progression ALI. Specific inhibition this pathway has been shown to alleviate ALI symptoms. Kaempferol-3-O-α-L-(4″-E-p-coumaroyl)-rhamnoside (KAE), an active compound found in flowers Angelica acutiloba Kitagawa, exhibits anti-inflammatory antioxidant properties. This study aimed investigate molecular mechanisms through KAE regulates cGAS-STING context was induced using LPS. Lung damage anti-inflammatory/antioxidant effects were assessed H&E staining, edema index, SOD, MDA, ELISA assays. NO release mitochondrial membrane potential (MMP) measured JC-1 Griess methods. The impact on PANoptosis analyzed flow cytometry, Western blot, immunofluorescence. significantly alleviated lipopolysaccharide-induced pulmonary reducing inflammatory cell infiltration, alleviating edema, enhancing capacity, decreasing levels cytokines mouse tissues. In both vitro vivo analyses, downregulated expression key components including cGAS, STING, p-TBK1, nuclear factor-κB. also reduced assembly PANoptosome, thereby attenuating apoptosis, necroptosis, pyroptosis. Additionally, inhibited cGAS restoring MMP, cytosolic DNA. improve inhibiting DNA suppressing activation, protecting cells from PANoptosis. Our findings provide valuable insights for development application novel therapeutic strategies

Language: Английский

Citations

0
Regulation of lncRNA-ENST on Myc-mediated mitochondrial apoptosis in mesenchymal stem cells: In vitro evidence implicated for acute lung injury therapeutic potential DOI
Yifeng Shen,

G. Yang,

Qimin Ma

et al.

World Journal of Stem Cells, Journal Year: 2025, Volume and Issue: 17(3)

Published: March 19, 2025

Acute lung injury (ALI) is a fatal and heterogeneous disease. While bone marrow mesenchymal stem cells (BMSCs) have shown promise in ALI repair, their efficacy compromised by high apoptotic percentage. Preliminary findings indicated that long noncoding RNA (lncRNA)-ENST expression markedly downregulated MSCs under ischemic hypoxic conditions, establishing rationale for vitro exploration. To elucidate the role of lncRNA-ENST00000517482 (lncRNA-ENST) modulating MSC apoptosis. Founded on BEAS-2B with lipopolysaccharide, this study employed transwell co-culture system to BMSC tropism. BMSCs were genetically modified overexpress or knockdown lncRNA-ENST. After analyzing effects autophagy, apoptosis cell viability, lncRNA-ENST/miR-539/c-MYC interaction was confirmed dual-luciferase assays. These revealed strong correlation between lncRNA-ENST levels autophagic status BMSCs. On one hand, over-expression lncRNA-ENST, as determined Cell Counting Kit-8 assays, increased autophagy markers LC3B, ATG7, ATG5. other it reduced boosted viability. In co-cultures cells, overexpression also improved vitality. Additionally, downregulating miR-539 upregulating c-MYC, found influence mitochondrial membrane potential, enhance mitigate lower secretion pro-inflammatory cytokines interleukin-6 interleukin-1β. Collectively, within framework, these results highlighted therapeutic potential pivotal regulatory lipopolysaccharide-stimulated cells. Our show enhanced lncRNA ENST can promote proliferation viability miR-539/c-MYC axis, reduce induce which has suggested its treatment ALI.

Language: Английский

Citations

0
Unraveling the protective role of Nrf2 in molluscs: Insights into mitochondrial and apoptosis pathways in the defense against Bap-induced oxidative stress DOI

Longmei Qiu,

Xinglu Chen,

Baoying Guo

et al.

Aquatic Toxicology, Journal Year: 2023, Volume and Issue: 264, P. 106728 - 106728

Published: Oct. 11, 2023

Language: Английский

Citations

6
Betanin protects against bleomycin-induced pulmonary fibrosis by regulating the NLRP3/IL-1β/TGF-β1 pathway-mediated epithelial-to-mesenchymal transition DOI

Nesma A. Abd Elrazik,

Sahar A. Helmy

Food & Function, Journal Year: 2023, Volume and Issue: 15(1), P. 284 - 294

Published: Dec. 12, 2023

Idiopathic pulmonary fibrosis (IPF) is a life-threatening disease that leads to dyspnea and progressive loss of lung function. This study aimed investigate the protective effect betanin (BET), major pigment in red beetroot, on induced by bleomycin (BLM) rats assess underlying mechanisms. In this view, total differential cell counts LDH activity bronchoalveolar lavage fluid were estimated. Furthermore, MDA GSH contents lungs colorimetrically measured, while hydroxyproline, NLRP3, ASC, caspase-1, TGF-β1, vimentin levels tissue evaluated using ELISA technique. Moreover, IL-1β, E-cadherin, α-SMA expressions analyzed immunostaining specimens. BET treatment protects against as indicated reduction counts, activity, TGF-β1 levels. content was also decreased following administration, elevated. Additionally, suppressed EMT process evidenced an increase E-cadherin expression besides expressions. To conclude, these results revealed might be attributed attenuation NLRP3/IL-1β/TGF-β1 signaling pathway process.

Language: Английский

Citations

4
Relationship between ferroptosis and mitophagy in acute lung injury: a mini-review DOI Creative Commons

Yunhua Cheng,

Liling Zhu,

Shuangxiong Xie

et al.

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e18062 - e18062

Published: Sept. 10, 2024

Acute lung injury (ALI) is one of the most deadly and prevalent diseases in intensive care unit. Ferroptosis mitophagy are pathological mechanisms ALI. aggravates ALI, whereas regulates both closely related to reactive oxygen species (ROS). Mitophagy can regulate ferroptosis, but specific relationship between ferroptosis still unclear. This study summarizes previous research findings on mitophagy, revealing their involvement Examining functions mTOR NLPR3 helps clarify connection with goal establishing a theoretical foundation for potential therapeutic approaches future management

Language: Английский

Citations

1
Hexavalent chromium exposure induces lung injury via activation of NLRP3 and AIM2 inflammasomes in rats DOI Creative Commons
Ningning Li, Saifei Li, Yue Yu

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 286, P. 117181 - 117181

Published: Oct. 22, 2024

Language: Английский

Citations

1
Angelica acutiloba Kitagawa flower induces A549 cell pyroptosis via the NF-κB/NLRP3 pathway for anti-lung cancer effects DOI Creative Commons

Yonghu Chen,

Fangying Zhu,

Xian‐Hua Che

et al.

Cell Division, Journal Year: 2023, Volume and Issue: 18(1)

Published: Nov. 1, 2023

Abstract Angelica acutiloba Kitagawa, a traditional medicinal herb of the Umbelliferae family, has been demonstrated to have anticancer activity. In this study, we investigated anti-lung cancer effects two compounds extracted from A. flowers: kaempferol-3- O - α -L-(4″- E -p-coumaroyl)-rhamnoside (KAE) and platanoside (PLA). MTT, cell colony formation, migration (scratch) assays revealed that both KAE (100 μM) PLA (50 μM 100 inhibited viability, proliferation, A549 cells. Dichlorodihydrofluorescein diacetate showed also induced generation reactive oxygen species in Morphologically, cells swelled grew larger under treatment with PLA, most significant changes at PLA. Fluorescence staining measurement lactate dehydrogenase release underwent pyroptosis concomitant upregulation interleukin (IL)-1β IL-18. Furthermore, NF-κB, PARP, NLRP3, ASC, cleaved-caspase-1, GSDMD expression Subsequent investigations unveiled these interact augment NLRP3’s binding affinity stimulate assembly inflammasome, thereby inducing pyroptosis. conclusion, active components flower extract, had activities exerted through regulation proteins related NLRP3 inflammasome pathway.

Language: Английский

Citations

2
Age-related disease: Lungs DOI

Joshua O. Owuor,

Ayman O. Soubani

Aging, Journal Year: 2024, Volume and Issue: unknown, P. 165 - 174

Published: Jan. 1, 2024

Advanced age has been correlated with the development of a wide range diseases as described in previous chapters. The respiratory system, comprised lungs and accompanying pulmonary vascular structures, thoracic cage, diaphragm is unfortunately not exempt. lung third largest organ by mass humans, after skin liver. It integral facilitating vital gas exchange form oxygen, nitrogen, carbon dioxide between body outside world, serves reservoir for blood, maintains body's acid–base balance concert kidneys, process that to function fundamental unit each body, cell. In essence, it supports life we know it.

Language: Английский

Citations

0