Immunity Inflammation and Disease,
Journal Year:
2024,
Volume and Issue:
12(11)
Published: Nov. 1, 2024
ABSTRACT
Background
Intervertebral
disc
degeneration
(IDD)
is
a
major
cause
for
low
back
pain.
Studies
showed
the
association
between
senescence
and
degenerative
diseases.
Cell
can
promote
occurrence
development
of
diseases
through
multiple
mechanisms
including
inflammatory
stress,
oxidative
stress
nutritional
deprivation.
The
roles
senescence‐associated
genes
(SAGs)
remains
unknown
in
IDD.
Methods
Four
differently
expressed
SAGs
were
identified
as
hub
using
“limma“
package
R.
We
then
calculated
immune
infiltration
IDD
patients,
investigated
relation
infiltration.
Enrichment
analysis
was
performed
to
explore
functions
Nomogram
LASSO
model
based
on
constructed
predict
risk
severe
(SD)
patients.
Subsequently,
single
cell
conducted
describe
expression
pattern
intervertebral
tissue.
Results
ASPH,
CCND1,
IGFBP3
SGK1
SAGs.
Further
demonstrated
that
might
mediate
by
regulating
pathways.
four
good
performance
predicting
SD.
Single
revealed
CCND1
mainly
nucleus
pulposus
cells,
while
epithelial
cells.
Eleven
candidate
drugs
targeting
SAGS
predicted
patients
Comparative
Toxicogenomics
Database
(CDT).
PCR
immunohistochemical
levels
higher
SD
than
MD
(mild
degeneration)
Conclusions
comprehensive
IDD,
which
their
Based
SAGs,
we
established
predictive
explored
potential
drugs.
These
findings
provide
new
understandings
SAG
mechanism
promising
therapeutic
strategies
Aging,
Journal Year:
2024,
Volume and Issue:
16(13), P. 10868 - 10881
Published: June 20, 2024
As
a
common
disease,
cervical
spondylosis
(CS)
results
from
the
degeneration
of
intervertebral
disc.
However,
there
are
still
no
effective
clinical
strategies
for
treatment
this
disease.
Needle-scalpel
(Ns),
therapy
guided
by
traditional
Chinese
medicine
theory,
alleviates
disc
degradation
and
is
widely
used
in
clinic
to
treat
CS.
Stromal
cell-derived
factor-1
(SDF-1)
its
receptor
CXC
4
(CXCR4)
nucleus
pulposus
cells
play
an
important
role
CS
onset
development.
This
study
aimed
explore
whether
Ns
can
relieve
pain
regulate
SDF-1/CXCR4
axis
inhibit
apoptosis,
thereby
delaying
rat
model
It
was
found
that
Ns-treated
groups
exhibited
higher
mechanical
allodynia
scores
than
group,
H&E
staining,
MRI,
scanning
electron
microscopy
revealed
inhibited
degeneration.
Additionally,
significantly
increases
RNA
protein
expression
levels
SDF-1
CXCR4.
Furthermore,
these
treatments
alleviated
apoptosis
cells,
which
manifested
as
decline
proportion
apoptotic
inhibition
decrease
Bcl-2/Bax.
These
findings
indicated
mitigated
CS-induced
pain,
rats.
effects
may
be
mediated
specifically
regulating
signaling
axis.
Based
on
findings,
we
conclude
might
serve
promising
Immunity Inflammation and Disease,
Journal Year:
2024,
Volume and Issue:
12(11)
Published: Nov. 1, 2024
ABSTRACT
Background
Intervertebral
disc
degeneration
(IDD)
is
a
major
cause
for
low
back
pain.
Studies
showed
the
association
between
senescence
and
degenerative
diseases.
Cell
can
promote
occurrence
development
of
diseases
through
multiple
mechanisms
including
inflammatory
stress,
oxidative
stress
nutritional
deprivation.
The
roles
senescence‐associated
genes
(SAGs)
remains
unknown
in
IDD.
Methods
Four
differently
expressed
SAGs
were
identified
as
hub
using
“limma“
package
R.
We
then
calculated
immune
infiltration
IDD
patients,
investigated
relation
infiltration.
Enrichment
analysis
was
performed
to
explore
functions
Nomogram
LASSO
model
based
on
constructed
predict
risk
severe
(SD)
patients.
Subsequently,
single
cell
conducted
describe
expression
pattern
intervertebral
tissue.
Results
ASPH,
CCND1,
IGFBP3
SGK1
SAGs.
Further
demonstrated
that
might
mediate
by
regulating
pathways.
four
good
performance
predicting
SD.
Single
revealed
CCND1
mainly
nucleus
pulposus
cells,
while
epithelial
cells.
Eleven
candidate
drugs
targeting
SAGS
predicted
patients
Comparative
Toxicogenomics
Database
(CDT).
PCR
immunohistochemical
levels
higher
SD
than
MD
(mild
degeneration)
Conclusions
comprehensive
IDD,
which
their
Based
SAGs,
we
established
predictive
explored
potential
drugs.
These
findings
provide
new
understandings
SAG
mechanism
promising
therapeutic
strategies
