Increased cell senescence in human metabolic disorders

Journal of Clinical Investigation, Journal Year: 2023, Volume and Issue: 133(12)

Published: June 14, 2023

Cell senescence (CS) is at the nexus between aging and associated chronic disorders, increases burden of CS in all major metabolic tissues. However, also increased adult obesity, type 2 diabetes (T2D), nonalcoholic fatty liver disease independent aging. Senescent tissues are characterized by dysfunctional cells inflammation, both progenitor mature, fully differentiated nonproliferating afflicted. Recent studies have shown that hyperinsulinemia insulin resistance (IR) promote human adipose cells. Similarly, promotes cellular IR, showing their interdependence. Furthermore, T2D age, BMI, degree hyperinsulinemia, suggesting premature These results suggest senomorphic/senolytic therapy may become important for treating these common disorders.

Language: Английский

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Protocol for the three-dimensional analysis of rodent skeletal muscle

STAR Protocols, Journal Year: 2025, Volume and Issue: 6(1), P. 103549 - 103549

Published: Jan. 10, 2025

Language: Английский

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Scalable production of muscle and adipose cell-laden microtissues using edible macroporous microcarriers for 3D printing of cultured fish fillets

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 18, 2025

Cellular agriculture is a novel platform for addressing the issues of protein scarcity, environmental pressures, and food safety. However, expanding seed cells at large scale remains prerequisite achieving industrial cultured meat production. We here propose production large-pore-sized edible porous microcarriers (EPMs) by using NaCl to precisely control ice crystal growth during cryogenic crosslinking. Muscle satellite (SCs) adipose-derived stem (ASCs) from yellow croakers exhibit remarkable adhesion, proliferation, differentiation on gelatin-based EPMs. Following consecutive expansion, SCs ASCs densities reach 6.25 × 105 5.77 cells/mL, leading 499-fold 461-fold increase in cell numbers, respectively. Subsequently, mature microtissues are incorporated into bioink, thereby enabling successful three-dimensional printing fish fillets with sensory properties similar native fillets. EPM-based expansion structuring techniques promising facilitating large-scale Cell big challenge growing field cellular agriculture. Here, authors present an approach muscle adipose cell-laden 3D

Language: Английский

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Stem Cell Aging in Skeletal Muscle Regeneration and Disease

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(5), P. 1830 - 1830

Published: March 6, 2020

Skeletal muscle comprises 30–40% of the weight a healthy human body and is required for voluntary movements in humans. Mature skeletal formed by multinuclear cells, which are called myofibers. Formation myofibers depends on proliferation, differentiation, fusion progenitor cells during development after injury. Muscle derived from satellite (stem) (MuSCs), reside surface myofiber but beneath basement membrane. MuSCs play central role postnatal maintenance, growth, repair, regeneration muscle. In sedentary adult muscle, mitotically quiescent, promptly activated response to Physiological chronological aging induces MuSC aging, leading an impaired regenerative capability. Importantly, pathological situations, repetitive injury early impairment due stem cell leads ability. this review, we discuss (1) regeneration, (2) under physiological conditions, (3) prospects related clinical applications controlling MuSCs.

Language: Английский

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Muscle stem cell isolation and in vitro culture for meat production: A methodological review

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2020, Volume and Issue: 20(1), P. 429 - 457

Published: Nov. 6, 2020

Abstract Cultured muscle tissue‐based protein products, also known as cultured meat, are produced through in vitro myogenesis involving stem cell culture and differentiation, mature processing for flavor texture. This review focuses on the meat production. The cell–based tissue production consists of a sequential process: (1) sampling collection, (2) dissociation isolation, (3) primary culture, (4) upscaled (5) differentiation maturation, (6) harvest. Although research is well‐established field, majority these steps remain to be underoptimized enable creation edible muscle‐derived products. profound understanding process would help not only but business sectors that have been seeking new biomaterials food industry. In this review, we discuss comprehensively detail each step cutting‐edge methods meaningful both academia industry prepare era cellular agriculture.

Language: Английский

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Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality

Cell Reports, Journal Year: 2021, Volume and Issue: 35(10), P. 109223 - 109223

Published: June 1, 2021

During aging, the regenerative capacity of skeletal muscle decreases due to intrinsic changes in stem cells (MuSCs) and alterations their niche. Here, we use quantitative mass spectrometry characterize MuSC proteome remodeling niche during aging. We generate a network connecting age-affected ligands located cell surface receptors on MuSCs. Thereby, reveal signaling by integrins, Lrp1, Egfr, Cd44 as major communication axes perturbed through investigate effect Smoc2, secreted protein that accumulates with primarily originating from fibro-adipogenic progenitors. Increased levels Smoc2 contribute aberrant Integrin beta-1 (Itgb1)/mitogen-activated kinase (MAPK) observed thereby causing impaired functionality regeneration. By MuSCs niche, our work will enable better understanding how are affected

Language: Английский

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Biological Aspects of Selected Myokines in Skeletal Muscle: Focus on Aging

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(16), P. 8520 - 8520

Published: Aug. 7, 2021

In the last decade, clear evidence has emerged that cellular components of skeletal muscle are important sites for release proteins and peptides called “myokines”, suggesting plays role a secretory organ. After their secretion by muscles, these factors serve many biological functions, including exertion complex autocrine, paracrine and/or endocrine effects. sum, myokines affect multi-organ processes, such as trophism, metabolism, angiogenesis immunological response to different physiological (physical activity, aging, etc.) or pathological states (cachexia, dysmetabolic conditions, chronic inflammation, etc.). The aim this review is describe in detail number are, varying degrees, involved aging processes belong group present functional environment surrounding cell known “Niche”. particular described those that, acting both from within an autocrine manner, have defined relationship with modulation oxidative stress cells (mature stem) regulatory (metabolic regenerative) aging. Myostatin, IGF-1, NGF, S100 irisin examples specific peculiar features mechanisms action. particular, potential one most recently characterized myokines—irisin, directly linked active lifestyle—in reducing if not reversing senescence-induced damage discussed terms its possible application agent able counteract deleterious effects

Language: Английский

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ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion

Developmental Cell, Journal Year: 2021, Volume and Issue: 56(24), P. 3349 - 3363.e6

Published: Dec. 1, 2021

Myoblast fusion is essential for muscle development and regeneration. Yet, it remains poorly understood how mononucleated myoblasts fuse with preexisting fibers. We demonstrate that ERK1/2 inhibition (ERKi) induces robust differentiation of primary mouse through a linear pathway involving RXR, ryanodine receptors, calcium-dependent activation CaMKII in nascent myotubes. results myotube growth via at fusogenic synapse. Mechanistically, interacts regulates MYMK Rac1, CaMKIIδ/γ knockout mice exhibit smaller regenerated myofibers following injury. In addition, the expression dominant negative inhibits formation large multinucleated Finally, we evolutionary conservation chicken myoblasts. conclude represses signaling cascade leading to CaMKII-mediated myotubes, providing an attractive target cultivated meat industry regenerative medicine.

Language: Английский

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Efficient Myogenic/Adipogenic Transdifferentiation of Bovine Fibroblasts in a 3D Bioprinting System for Steak‐Type Cultured Meat Production

Advanced Science, Journal Year: 2022, Volume and Issue: 9(31)

Published: Oct. 3, 2022

The interest in cultured meat is increasing because of the problems with conventional livestock industry. Recently, many studies related to have been conducted, but producing large-sized remains a challenge. It aimed introduce 3D bioprinting for large cell aggregates production. A hydrogel scaffold produced at centimeter scale using bioink consisting photocrosslinkable materials digital light processing-based (DLP) printing, which has high printing accuracy and can produce geometrically complex structures. exposure time photopolymerization by DLP optimized based on photorheometry viability assays. Naturally immortalized bovine embryonic fibroblast cells transformed MyoD PPARγ2 instead primary are used as latter difficulties maintaining stemness associated animal ethics issues. mixed into printing. Myogenesis adipogenesis induced simply changing medium after Scaffolds obtained successfully living microchannels. cooked maintains its size shape upon cutting. overall dimensions 3.43 cm × 5.53 0.96 cm. This study provides proof-of-concept bioinks.

Language: Английский

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Engineered meatballs via scalable skeletal muscle cell expansion and modular micro-tissue assembly using porous gelatin micro-carriers

Biomaterials, Journal Year: 2022, Volume and Issue: 287, P. 121615 - 121615

Published: June 3, 2022

Language: Английский

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