Cultured meat platform developed through the structuring of edible microcarrier-derived microtissues with oleogel-based fat substitute

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: May 23, 2023

Abstract With the increasing global demand for meat, cultured meat technologies are emerging, offering more sustainable solutions that aim to evade a future shortage of meat. Here, we demonstrate platform composed edible microcarriers and an oleogel-based fat substitute. Scalable expansion bovine mesenchymal stem cells on chitosan-collagen is optimized generate cellularized microtissues. In parallel, oleogel system incorporated with plant protein developed as substitute, which comparable beef in appearance texture. Combining microtissues two types prototypes introduced: layered burger-like While prototype benefits enhanced stiffness, has marbling meat-like softer Overall, this established technological basis may contribute development different products promote their commercial production.

Language: Английский

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Review: Will “cultured meat” transform our food system towards more sustainability?

animal, Journal Year: 2024, Volume and Issue: unknown, P. 101145 - 101145

Published: March 1, 2024

Our agri-food system today should provide enough healthy food of good quality for the growing human population. However, it also preserve natural resources and better protect livestock. In this context, some FoodTech companies are developing a disruptive approach: cell culture in vitro production "meat" but technology is still at research development stage. This article will highlight its development, technologies used stakeholders involved (Part 1), potential environmental impacts 2) regulatory, social ethical issues 3). aims to shed light throughout manuscript on two major controversies related "cultured meat". The first controversy aspects, which includes different points: reduce animal suffering therefore improve welfare, future values our society, trend towards artificialisation. second environmental, health nutritional issues, relation characteristics meat" with an important question: we call meat? These act interaction association societal, legal consequently political issues. Answers various questions depend visions World by stakeholders, consumers citizens. Some them argue moderate or strong reduction livestock farming, even abolition farming perceived as exploitation farm animals. Others only want current much criticised intensive/industrial model. Compared other sustainable solutions be implemented such losses waste, new consumption habits less proteins sources, intensification, agroecological production, market meat substitutes (proteins from plants, mycoproteins, algae, insects, etc.), has uncertain future.

Language: Английский

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Growing meat on autoclaved vegetables with biomimetic stiffness and micro-patterns

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 2, 2025

Cultured meat needs edible bio-scaffolds that provide not only a growth milieu for muscle and adipose cells, but also biomimetic stiffness tissue-sculpting topography. Current meat-engineering technologies struggle to achieve scalable cell production, efficient differentiation, tissue maturation in one single culture system. Here we propose an autoclaving strategy transform common vegetables into muscle- adipose-engineering scaffolds, without undergoing conventional plant decellularization. We selected with natural anisotropic isotropic topology mimicking microstructures respectively. further adjusted vegetable by autoclaving, emulate the mechanical properties of animal tissues. Autoclaved preserve rich cell-affinitive moieties, yielding good effect simplified processing. Chinese chive Shiitake mushroom micro-patterns support expansion improved alignment myogenesis. loofah encourages adipocyte proliferation lipid accumulation. Our engineered fat-on-vegetables can construct stuffing or layered chips. possess tissue-mimicking topology, bring biochemical benefits, operational ease, cost reduction bioreactor compatibility. Without needing decellularization, these biomaterials may see scale-up applications analog bio-fabrication. Traditional cultured scaffolds employ animal-derived intricate manufacturing, plant-based offer appealing alternative. authors present simple shortcut method bioscaffolds via which mimics microstructure.

Language: Английский

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Engineered Living Systems Based on Gelatin: Design, Manufacturing, and Applications

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Engineered living systems (ELSs) represent purpose-driven assemblies of components, encompassing cells, biomaterials, and active agents, intricately designed to fulfill diverse biomedical applications. Gelatin its derivatives have been used extensively in ELSs owing their mature translational pathways, favorable biological properties, adjustable physicochemical characteristics. This review explores the intersection gelatin with fabrication techniques, offering a comprehensive examination synergistic potential creating for various applications biomedicine. It offers deep dive into gelatin, including structures production, sources, processing, properties. Additionally, techniques employing derivatives, generic microfluidics, 3D printing methods. Furthermore, it discusses based on regenerative engineering as well cell therapies, bioadhesives, biorobots, biosensors. Future directions challenges are also examined, highlighting emerging trends areas improvements innovations. In summary, this underscores significance gelatin-based advancing lays groundwork guiding future research developments within field.

Language: Английский

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Scalable production of muscle and adipose cell-laden microtissues using edible macroporous microcarriers for 3D printing of cultured fish fillets

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 18, 2025

Cellular agriculture is a novel platform for addressing the issues of protein scarcity, environmental pressures, and food safety. However, expanding seed cells at large scale remains prerequisite achieving industrial cultured meat production. We here propose production large-pore-sized edible porous microcarriers (EPMs) by using NaCl to precisely control ice crystal growth during cryogenic crosslinking. Muscle satellite (SCs) adipose-derived stem (ASCs) from yellow croakers exhibit remarkable adhesion, proliferation, differentiation on gelatin-based EPMs. Following consecutive expansion, SCs ASCs densities reach 6.25 × 105 5.77 cells/mL, leading 499-fold 461-fold increase in cell numbers, respectively. Subsequently, mature microtissues are incorporated into bioink, thereby enabling successful three-dimensional printing fish fillets with sensory properties similar native fillets. EPM-based expansion structuring techniques promising facilitating large-scale Cell big challenge growing field cellular agriculture. Here, authors present an approach muscle adipose cell-laden 3D

Language: Английский

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Gellan gum-gelatin scaffolds with Ca2+ crosslinking for constructing a structured cell cultured meat model

Biomaterials, Journal Year: 2023, Volume and Issue: 299, P. 122176 - 122176

Published: May 22, 2023

Language: Английский

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Cell-based meat: The molecular aspect

Frontiers in Food Science and Technology, Journal Year: 2023, Volume and Issue: 3

Published: March 30, 2023

Cellular agriculture is one of the evolving fields translational biotechnology. The emerging science aims to improve issues related sustainable food products and security, reduce greenhouse gas emissions provide animal wellbeing by circumventing livestock farming through cell-based meat (CBM) production. CBM exploits cell culture techniques biomanufacturing methods manipulating mammalian, avian, fish lines. ought successfully meet demand for nutritional protein human consumption pet animals. However, substantial advancement modification are required manufacturing in terms cost, palatability, consumer acceptance, safety. In order achieve high-quality its production with high yield, molecular aspect needs a thorough inspection good laboratory practices commercial current review discusses various aspects biology involved establishing lines, myogenesis, regulation, scaffold, bioreactor-related approaches target CBM.

Language: Английский

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Generation of three-dimensional meat-like tissue from stable pig epiblast stem cells

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 9, 2023

Abstract Cultured meat production has emerged as a breakthrough technology for the global food industry with potential to reduce challenges associated environmental sustainability, public health, animal welfare, and competition between humans animals. The muscle stem cell lines currently used cultured cannot be passaged in vitro extended periods of time. Here, we develop directional differentiation system porcine pre-gastrulation epiblast cells (pgEpiSCs) stable cellular features achieve serum-free myogenic pgEpiSCs. We show that pgEpiSCs-derived skeletal progenitor fibers have typical characteristics display transcriptional during differentiation. Importantly, establish three-dimensional shaping tissue by screening plant-based edible scaffolds non-animal origin, followed generation meat. These advances provide technical approach development

Language: Английский

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Animal-free scaffold from brown algae provides a three-dimensional cell growth and differentiation environment for steak-like cultivated meat

Food Hydrocolloids, Journal Year: 2024, Volume and Issue: 152, P. 109944 - 109944

Published: Feb. 26, 2024

Scaffolds for the production of cultivated meat, a promising sustainable meat alternative, should exhibit physical and chemical properties that enable three-dimensional animal cell culture, along with biological characteristics support attachment, proliferation, differentiation. Additionally, scaffold be crafted from edible materials offer textural similarities to have minimal influence on flavor taste. Herein, an alginate-based alginate-cellulose hydrogel (ACe-gel) derived brown alga Undaria pinnatifida is developed. In terms characteristics, had porosity (119.5 ± 37.2 μm) moisture-holding capacity (73.03 3.82, 68.66 9.54, 84.17 9.94 at 25 °C, 37 60 respectively) suitable culture differentiation bovine-derived muscle stem cells (bMuSCs). Accordingly, was superior commercial alginate in attachment proliferation bMuSCs (5.5-fold over 72 h), its performance comparable lyophilized collagen (7.8-fold h, compared pure alginate). The cultured ACe-gel were capable differentiating into fibers, as verified by gene expression profile analysis. Furthermore, exhibited heavy metal contents distinct seaweed odorants, while stress-strain bMuSC (Young's modulus raw ACe-gel: 285.19 83.37 kPa, cooked meat: 880.60 485.60 kPa) closely resembled (raw beef: 267.76 156.42 1331.94 762.43 kPa). These findings highlight seaweed-derived animal-free has strong potential utilization food technology future.

Language: Английский

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3D Printing of Maturable Tissue Constructs Using a Cell‐Adaptable Nanocolloidal Hydrogel

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(28)

Published: May 8, 2024

Abstract 3D‐printed cell‐laden hydrogels as tissue constructs show great promise in generating living tissues for medicine. Currently, the maturation of into remains challenge, since commonly used struggle to provide an ideal microenvironment seeded cells. In this study, a cell‐adaptable nanocolloidal hydrogel is created 3D printing maturable constructs. The composed interconnected nanoparticles, which prepared by self‐assembly and subsequent photocrosslinking gelatin methacryloyl solutions. Cells can get enough space grow migrate within through squeezing flexible networks. Meanwhile, nanostructure promote cells proliferate produce matrix proteins mechanotransduction. Using digital light process‐based technology, it rapidly customize cartilage After implantation, these efficiently matured articular defect repair ear reconstruction vivo. using shows potential future clinical applications.

Language: Английский

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