Melatonin Enhances Heat Tolerance via Increasing Antioxidant Enzyme Activities and Osmotic Regulatory Substances by Upregulating zmeno1 Expression in Maize (Zea mays L.) DOI Creative Commons
Liru Cao, Abbas Muhammad Fahim,

Xiaohan Liang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(9), P. 1144 - 1144

Published: Sept. 22, 2024

Heat stress severely affects the yield and quality of maize. Melatonin (N-acetyl-5-methoxy-tryptamin, MT) plays an important role in various types resistance plants, including heat tolerance. Enolase (ENO, 2-phospho-D-glycerate hydrolyase) contributes to plant growth, development, response. As now, molecular mechanisms by which MT ENO1 affect tolerance are unknown. In our research, we have revealed that (H) + (MH) treatment upregulate ZmENO1 expression levels 15 20 times, respectively. overexpression mutant maize lines were created transgenic genome editing. These results illustrate has a significant impact on growth at seedling stage. However, ZmENO1-OE showed lower degree susceptibility stress, whereas exhibited most severe effects. Under exogenous application improves The best highest survival rate, while zmeno1 mutants least desirable results. Following with H MH, level greatest increase reached maximum value, was lowest. decreased maize’s relative water content fresh weight, although had led MDA, hydrogen peroxide, superoxide all plants. Additionally, ionic permeability osmotic potential plants significantly increased. decrease observed lines. Interestingly, MT. stress-induced upregulation ZmSOD, ZmPOD, ZmAPX, ZmCAT, ZmP5CS, ZmProDH levels, lowest following spraying. patterns SOD, POD, APX, CAT enzyme activity, as well proline soluble protein content, aligned variations these genes. Our findings indicate can gene. Upregulating gene resulted elevated ZmProDH. This increased activity antioxidant enzymes higher osmoregulatory substances. Consequently, it mitigated cell membrane damage caused ultimately improved this study provide genetic resources for design breeding lay solid foundation further exploring mechanism regulation

Language: Английский

Phenotyping, genome‐wide dissection, and prediction of maize root architecture for temperate adaptability DOI Creative Commons
Weijun Guo, Fanhua Wang, Jiaping Lv

et al.

iMeta, Journal Year: 2025, Volume and Issue: 4(2)

Published: March 13, 2025

Root System Architecture (RSA) plays an essential role in influencing maize yield by enhancing anchorage and nutrient uptake. Analyzing RSA dynamics holds potential for ideotype-based breeding prediction, given the limited understanding of genetic basis maize. Here, we obtained 16 root morphology-related traits (R-traits), 7 weight-related (W-traits), 108 slice-related microphenotypic (S-traits) from meristem, elongation, mature zones cross-sectioning primary, crown, lateral roots 316 lines. Significant differences were observed some between tropical/subtropical temperate lines, such as primary total diameters, lengths, area. Additionally, anatomy data integrated with genome-wide association study (GWAS) to elucidate architecture complex traits. GWAS identified 809 genes associated R-traits, 261 linked W-traits, 2577 key related We confirm function a candidate gene, fucosyltransferase5 (FUT5), regulating development heat tolerance The different FUT5 haplotypes found lines are features hold promising applications molecular breeding. Furthermore, performed machine learning prediction models using slice traits, achieving high accuracy. Collectively, our offers valuable tool dissecting RSA, along resources predictive beneficial design enhancement.

Language: Английский

Citations

0
Melatonin enhances salt tolerance by promoting CcCAD10-mediated lignin biosynthesis in pigeon pea DOI
Feng Pan, Hongquan Li, Ming Qu

et al.

Theoretical and Applied Genetics, Journal Year: 2025, Volume and Issue: 138(4)

Published: March 22, 2025

Language: Английский

Citations

0
Identification of the maize drought-resistant gene Zinc-finger Inflorescence Meristem 23 through high-resolution temporal transcriptome analysis DOI
Tangnur Kaderbek, Liangliang Huang,

Yang Yue

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142347 - 142347

Published: March 1, 2025

Language: Английский

Citations

0
Genome-Based Identification of the Dof Gene Family in Three Cymbidium Species and Their Responses to Heat Stress in Cymbidium goeringii DOI Open Access

Xin He,

Meng-Meng Zhang, Ye Huang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7662 - 7662

Published: July 12, 2024

As an important genus in Orchidaceae, Cymbidium has rich ecological diversity and significant economic value. DNA binding with one zinc finger (Dof) proteins are pivotal plant-specific transcription factors that play crucial roles the growth, development, stress response of plants. Although Dof genes have been identified functionally analyzed numerous plants, exploration Orchidaceae remains limited. We conducted a thorough analysis gene family goeringii, C. ensifolium, sinensis. In total, 91 (27 CgDofs, 34 CeDofs, 30 CsDofs) were identified, divided into five groups (I–V) based on phylogenetic analysis. All motif 1 2 conserved domains over half contained introns. Chromosomal localization collinearity revealed their evolutionary relationships potential duplication events. Analysis cis-elements CsDofs promoters showed light-responsive most common, followed by hormone-responsive elements, plant growth-related abiotic elements. three species primarily exhibit random coil structure, while homology modeling exhibited similarity. addition, RT-qPCR expression levels nine CgDofs changed greatly under heat stress. CgDof03, CgDof22, CgDof27, CgDof08, CgDof23 varying degrees upregulation. Most upregulated belong to group I, indicating I great for high-temperature resistance. conclusion, our study systematically demonstrated molecular characteristics different species, preliminarily patterns stress, provided reference further breeding orchids.

Language: Английский

Citations

2
Genome-Wide Identification of the DOF Gene Family in Kiwifruit (Actinidia chinensis) and Functional Validation of AcDOF22 in Response to Drought Stress DOI Open Access
Chao Zhao, Hao Bai, Chaoshuo Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9103 - 9103

Published: Aug. 22, 2024

DNA-binding one zinc finger (DOF) transcription factors are crucial plant-specific regulators involved in growth, development, signal transduction, and abiotic stress response generation. However, the genome-wide identification characterization of AcDOF genes their regulatory elements kiwifruit (Actinidia chinensis) has not been thoroughly investigated. In this study, we screened genome database identified 42 (AcDOF1 to AcDOF42). Phylogenetic analysis facilitated categorization these into five subfamilies (DOF-a, DOF-b, DOF-c, DOF-d, DOF-e). We further analyzed motifs, conserved domains, gene structures, collinearity AcDOFgene family. Gene ontology (GO) enrichment indicated significant “flower development” term “response stress” category. Promoter prediction revealed numerous cis-regulatory related responses light, hormones, low-temperature drought promoters. RNA-seq expression profiles demonstrated tissue-specific genes. Quantitative real-time PCR results showed that six selected (AcDOF04, AcDOF09, AcDOF11, AcDOF13, AcDOF21, AcDOF22) were differentially induced by abscisic acid (ABA), methyl jasmonate (MeJA), cold, salt, stresses, with AcDOF22 specifically expressed at high levels drought-tolerant cultivars. Further experiments transient overexpression leaf disks reduced water loss chlorophyll degradation. Additionally, was localized nucleus exhibited transcriptional activation, enhancing resistance activating downstream marker AcDREB2A. These findings lay foundation for elucidating molecular mechanisms offer new insights drought-resistant breeding.

Language: Английский

Citations

1
Melatonin Enhances Heat Tolerance via Increasing Antioxidant Enzyme Activities and Osmotic Regulatory Substances by Upregulating zmeno1 Expression in Maize (Zea mays L.) DOI Creative Commons
Liru Cao, Abbas Muhammad Fahim,

Xiaohan Liang

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(9), P. 1144 - 1144

Published: Sept. 22, 2024

Heat stress severely affects the yield and quality of maize. Melatonin (N-acetyl-5-methoxy-tryptamin, MT) plays an important role in various types resistance plants, including heat tolerance. Enolase (ENO, 2-phospho-D-glycerate hydrolyase) contributes to plant growth, development, response. As now, molecular mechanisms by which MT ENO1 affect tolerance are unknown. In our research, we have revealed that (H) + (MH) treatment upregulate ZmENO1 expression levels 15 20 times, respectively. overexpression mutant maize lines were created transgenic genome editing. These results illustrate has a significant impact on growth at seedling stage. However, ZmENO1-OE showed lower degree susceptibility stress, whereas exhibited most severe effects. Under exogenous application improves The best highest survival rate, while zmeno1 mutants least desirable results. Following with H MH, level greatest increase reached maximum value, was lowest. decreased maize’s relative water content fresh weight, although had led MDA, hydrogen peroxide, superoxide all plants. Additionally, ionic permeability osmotic potential plants significantly increased. decrease observed lines. Interestingly, MT. stress-induced upregulation ZmSOD, ZmPOD, ZmAPX, ZmCAT, ZmP5CS, ZmProDH levels, lowest following spraying. patterns SOD, POD, APX, CAT enzyme activity, as well proline soluble protein content, aligned variations these genes. Our findings indicate can gene. Upregulating gene resulted elevated ZmProDH. This increased activity antioxidant enzymes higher osmoregulatory substances. Consequently, it mitigated cell membrane damage caused ultimately improved this study provide genetic resources for design breeding lay solid foundation further exploring mechanism regulation

Language: Английский

Citations

1