Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Betula platyphylla Reveals Promising Candidates for Heat Tolerance DOI Open Access
Shengzhou Guo, Hao Chen, Hongwei Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 172 - 172

Published: Dec. 28, 2024

Heat stress transcription factors (HSFs) play a critical role in orchestrating cellular responses to elevated temperatures and various conditions. While extensively studied model plants, the HSF gene family Betula platyphylla remains unexplored, despite availability of its sequenced genome. In this study, we employed bioinformatics approaches identify 21 BpHSF genes within genome, revealing their uneven distribution across chromosomes. These were categorized into three subfamilies: A, B, C. Each was characterized by conserved protein motifs structures, with notable divergence observed between subfamilies. Collinearity analysis suggested that segmental duplication events have driven evolutionary expansion family. Promoter region identified an array cis-acting elements linked growth, development, hormonal regulation, responses. Subcellular localization experiments confirmed nuclear BpHSFA2a, BpHSFB1a, BpHSFC1a, consistent silico predictions. RNA-seq RT-qPCR analyses revealed tissue-specific expression patterns dynamic heat stress, qPCR validation highlighting significant upregulation BpHSFA2a under high-temperature summary, study provided comprehensive characterization platyphylla, laying solid foundation for future functional studies. Particularly, emerges as promising candidate enhancing tolerance warranting further detailed investigation.

Language: Английский

Identification and Characterization of the DOF Gene Family in Phoebe bournei and Its Role in Abiotic Stress—Drought, Heat and Light Stress DOI Open Access
Kehui Zheng,

Mengmeng Lv,

Jiaying Qian

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11147 - 11147

Published: Oct. 17, 2024

is a second-class endangered and protected species unique to China, it holds significant ecological economic value. DNA binding one zinc finger (Dof) transcription factors are plant-specific regulators. Numerous studies have demonstrated that

Language: Английский

Citations

1
Genome-Wide Characterization of the Heat Shock Transcription Factor Gene Family in Betula platyphylla Reveals Promising Candidates for Heat Tolerance DOI Open Access
Shengzhou Guo, Hao Chen, Hongwei Wu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 172 - 172

Published: Dec. 28, 2024

Heat stress transcription factors (HSFs) play a critical role in orchestrating cellular responses to elevated temperatures and various conditions. While extensively studied model plants, the HSF gene family Betula platyphylla remains unexplored, despite availability of its sequenced genome. In this study, we employed bioinformatics approaches identify 21 BpHSF genes within genome, revealing their uneven distribution across chromosomes. These were categorized into three subfamilies: A, B, C. Each was characterized by conserved protein motifs structures, with notable divergence observed between subfamilies. Collinearity analysis suggested that segmental duplication events have driven evolutionary expansion family. Promoter region identified an array cis-acting elements linked growth, development, hormonal regulation, responses. Subcellular localization experiments confirmed nuclear BpHSFA2a, BpHSFB1a, BpHSFC1a, consistent silico predictions. RNA-seq RT-qPCR analyses revealed tissue-specific expression patterns dynamic heat stress, qPCR validation highlighting significant upregulation BpHSFA2a under high-temperature summary, study provided comprehensive characterization platyphylla, laying solid foundation for future functional studies. Particularly, emerges as promising candidate enhancing tolerance warranting further detailed investigation.

Language: Английский

Citations

0