Downregulation of Vascular Hemeoxygenase-1 Leads to Vasculopathy in Systemic Sclerosis DOI Creative Commons
Rebecca L. Ross, Georgia Mavria, Francesco Del Galdo

et al.

Frontiers in Physiology, Journal Year: 2022, Volume and Issue: 13

Published: May 5, 2022

Systemic sclerosis (SSc) is a terminal disease characterized by vasculopathy, tissue fibrosis, and autoimmunity. Although the exact etiology of SSc remains unknown, endothelial dysfunction, oxidative stress, calcium handling dysregulation have been associated with large number SSc-related complications such as neointima formation, vasculogenesis, pulmonary arterial hypertension, impaired angiogenesis, cardiac arrhythmias. Hemeoxygenase-1 (HO-1) an antioxidant enzyme involved in multiple biological actions cardiovascular system including vascular tone, cellular proliferation, apoptosis, stress. The aim this work was to investigate physiological role HO-1 its relevance occurring SSc. We found that, early phases SSc, expression dermal fibroblast lower compared those isolated from healthy control individuals. This particularly relevant reduction HO-1/CO signaling pathway dysfunction vasculopathy. show evidence HO-1/carbon monoxide (CO) handling. Using vitro model hypertension (PAH) we investigated Ca2+ mobilization intracellular stores. Our results indicate that regulates release stores human cells. interrogated activity angiogenesis using organotypic co-culture fibroblast-endothelial cell. Inhibition significantly reduced ability cells form tubules. further if could be cell motility or migration into extracellular matrix synthesized fibroblasts. By mean holographic imaging, studied morphological functional features presence activator selective inhibitors. demonstrate inhibition reduces proliferation (migration) cultured cells, whilst activation does not modify either morphology, motility. In addition, CO on Kv7.1 (KCQN1) channel current, important component action potential repolarization. electrophysiology (whole-cell patch-clamp recombinant overexpressing KCQN1 channel), assessed regulation CO. CORM-2, donor, suggesting may play modulation via ion channel. summary, our clear link between: 1) downregulation signaling; 2) pathophysiological processes homeostasis dysregulation, A better understanding canonical (mainly due CO), non-canonical HO-1, well interaction other gasotransmitters will contribute development novel therapeutic approaches.

Language: Английский

Carbon monoxide therapy: a promising strategy for cancer DOI

Jingjing Chai,

Junfei Zhu,

Yu Tian

et al.

Journal of Materials Chemistry B, Journal Year: 2023, Volume and Issue: 11(9), P. 1849 - 1865

Published: Jan. 1, 2023

The present review introduced systematically a promising strategy for cancer: carbon monoxide therapy, and provided some valuable guidance promoting the progress of gas therapy nanomedicine.

Language: Английский

Citations

24
Development and mechanism of composite dust and toxic gas suppressant for tunnel blasting construction DOI

Menghui Xiao,

Cuifeng Du,

Yuan Wang

et al.

Powder Technology, Journal Year: 2025, Volume and Issue: 454, P. 120730 - 120730

Published: Feb. 1, 2025

Language: Английский

Citations

1
Ndufs4 knockout mouse models of Leigh syndrome: pathophysiology and intervention DOI Creative Commons
Melissa A.E. van de Wal, Merel J.W. Adjobo-Hermans, Jaap Keijer

et al.

Brain, Journal Year: 2021, Volume and Issue: 145(1), P. 45 - 63

Published: Nov. 22, 2021

Abstract Mitochondria are small cellular constituents that generate energy (ATP) by oxidative phosphorylation (OXPHOS). Dysfunction of these organelles is linked to a heterogeneous group multisystemic disorders, including diabetes, cancer, ageing-related pathologies and rare mitochondrial diseases. With respect the latter, mutations in subunit-encoding genes assembly factors first OXPHOS complex (complex I) induce isolated I deficiency Leigh syndrome. This syndrome an early-onset, often fatal, encephalopathy with variable clinical presentation poor prognosis due lack effective intervention strategies. Mutations nuclear DNA-encoded NDUFS4 gene, encoding NADH:ubiquinone oxidoreductase subunit S4 (NDUFS4) I, ‘mitochondrial deficiency, type 1’ (MC1DN1) paediatric patients. A variety (tissue-specific) Ndufs4 knockout mouse models were developed study pathomechanism testing. Here, we review discuss role human disease, how analysis has generated new insights into MC1ND1/Leigh its therapeutic targeting.

Language: Английский

Citations

53
Field measurements on the attenuation characteristics of PM2.5 and toxic gases in a blasting metro tunnel and evaluation of the re-entry time DOI

Zhuwei Xie,

Zhongtai Zhao,

Dingfu Li

et al.

Tunnelling and Underground Space Technology, Journal Year: 2023, Volume and Issue: 138, P. 105170 - 105170

Published: April 24, 2023

Language: Английский

Citations

15
Current trends in gas-synergized phototherapy for improved antitumor theranostics DOI Open Access
Ziwei Yan, Zhu Liu, Haotian Zhang

et al.

Acta Biomaterialia, Journal Year: 2023, Volume and Issue: 174, P. 1 - 25

Published: Dec. 12, 2023

Language: Английский

Citations

14
Carbon Monoxide-induced Autophagy Enhances Human Mesenchymal Stromal Cell Function via Paracrine Actions in Murine Polymicrobial Sepsis DOI Creative Commons
Narae Hwang, Sailaja Ghanta, Qifei Li

et al.

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(7), P. 2232 - 2247

Published: May 11, 2024

Sepsis is a life-threatening process due to organ dysfunction resulting from severe infections. Mesenchymal stromal cells (MSCs) are being investigated as therapy for sepsis, along with conditioning regimens improve their function. Carbon monoxide (CO) gas, which cytoprotective at low doses, induces autophagy and mediator of inflammation. We evaluated CO-induced in human MSCs (hMSCs), its impact on cell function murine cecal ligation puncture. Conditioning hMSCs CO ex vivo resulted enhanced survival bacterial clearance vivo, neutrophil phagocytosis bacteria vitro. Decreased infiltration less parenchymal death organs were associated increased macrophage efferocytosis apoptotic neutrophils, promoting resolution These effects lost when the exposed inhibition prior gas exposure. When assessing paracrine actions autophagy, extracellular vesicles (EVs) predominantly responsible. had no effect EV production, but altered miRNA cargo. Increased expression miR-145-3p miR-193a-3p by was blunted disruption inhibitors these miRNAs led loss efferocytosis. Collectively, hMSC during sepsis via MSC-derived EVs.

Language: Английский

Citations

5
Association of ambient carbon monoxide exposure with hospitalization risk for respiratory diseases: A time series study in Ganzhou, China DOI Creative Commons
Jiahao Song, Weihong Qiu,

Xuezan Huang

et al.

Frontiers in Public Health, Journal Year: 2023, Volume and Issue: 11

Published: Feb. 14, 2023

Ambient carbon monoxide (CO) exposure is associated with increased mortality and hospitalization risk for total respiratory diseases. However, evidence on the of specific diseases from ambient CO limited.Data daily hospitalizations diseases, air pollutants, meteorological factors January 2016 to December 2020 were collected in Ganzhou, China. A generalized additive model quasi-Poisson link lag structures was used estimate associations between concentration asthma, chronic obstructive pulmonary disease (COPD), upper tract infection (URTI), lower (LRTI), influenza-pneumonia. Possible confounding co-pollutants effect modification by gender, age, season considered.A 72,430 hospitalized cases recorded. Significant positive exposure-response relationships observed For each 1 mg/m3 increase (lag0-2), COPD, LRTI, influenza-pneumonia 13.56 (95% CI: 6.76%, 20.79%), 17.74 1.34%, 36.8%), 12.45 2.91%, 22.87%), 41.25 18.19%, 68.81%), 13.5% 3.41%, 24.56%), respectively. In addition, stronger during warm season, while women more susceptible exposure-associated asthma LRTI (all P < 0.05).In brief, significant found Effect gender hospitalizations.

Language: Английский

Citations

12
Single-Cell Analysis Reveals Cxcl14+ Fibroblast Accumulation in Regenerating Diabetic Wounds Treated by Hydrogel-Delivering Carbon Monoxide DOI Creative Commons
Ya Li, Lu Sun,

Ranxi Chen

et al.

ACS Central Science, Journal Year: 2024, Volume and Issue: 10(1), P. 184 - 198

Published: Jan. 2, 2024

Nonhealing skin wounds are a problematic complication associated with diabetes. Therapeutic gases delivered by biomaterials have demonstrated powerful wound healing capabilities. However, the cellular responses and heterogeneity in regeneration process after gas therapy remain elusive. Here, we display benefit of carbon monoxide (CO)-releasing hyaluronan hydrogel (CO@HAG) promoting diabetic investigate through single-cell transcriptomic analysis. The presented CO@HAG demonstrates microenvironment responsive releasing properties accelerates vivo. It is found that new cluster Cxcl14+ fibroblasts progenitor property accumulated CO@HAG-treated wound. This yet unreported process. macrophages feature decrease pro-inflammatory property, while their anti-inflammatory increases. Moreover, TGF-β signal between (M1) macrophage fibroblast attenuated based on cell–cell interaction Our study provides useful hydrogel-mediated method for insights into events gas-releasing therapy.

Language: Английский

Citations

4
Novelties on Neuroinflammation in Alzheimer’s Disease–Focus on Gut and Oral Microbiota Involvement DOI Open Access
Cristina Popescu, Constantin Munteanu, Aurelian Anghelescu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11272 - 11272

Published: Oct. 19, 2024

Recent studies underscore the role of gut and oral microbiota in influencing neuroinflammation through microbiota–gut–brain axis, including Alzheimer’s disease (AD). This review aims to provide a comprehensive synthesis recent findings on involvement neuroinflammatory processes associated with AD, emphasizing novel insights therapeutic implications. reveals that dysbiosis AD patients’ is linked heightened peripheral central inflammatory responses. Specific bacterial taxa, such as Bacteroides Firmicutes gut, well Porphyromonas gingivalis cavity, are notably altered leading significant changes microglial activation cytokine production. Gut alterations increased intestinal permeability, facilitating translocation endotoxins like lipopolysaccharides (LPS) into bloodstream exacerbating by activating brain’s toll-like receptor 4 (TLR4) pathways. Furthermore, microbiota-derived metabolites, short-chain fatty acids (SCFAs) amyloid peptides, can cross blood-brain barrier modulate While microbial amyloids may contribute amyloid-beta aggregation brain, certain SCFAs butyrate exhibit anti-inflammatory properties, suggesting potential avenue mitigate neuroinflammation. not only highlights critical pathology but also offers ray hope modulating could represent strategy for reducing slowing progression.

Language: Английский

Citations

4
Nanoparticles augment the therapeutic window of RT and immunotherapy for treating cancers: pivotal role of autophagy DOI Creative Commons

Yuan-Hua Wu,

Rong-Jane Chen, Hui‐Wen Chiu

et al.

Theranostics, Journal Year: 2022, Volume and Issue: 13(1), P. 40 - 58

Published: Nov. 29, 2022

Immunotherapies are now emerging as an efficient anticancer therapeutic strategy. Cancer immunotherapy utilizes the host's immune system to fight against cancer cells and has gained increasing interest due its durable efficacy low toxicity compared traditional antitumor treatments, such chemotherapy radiotherapy (RT). Although combination of RT drawn extensive attention in clinical setting, overall response rates still low. Therefore, strategies for further improvement urgently needed. Nanotechnology been used target not only but also tumor microenvironment (TME), thereby helping generate a long-term response. Nanomaterials can be effective delivery strong autophagy inducer, with ability elevate very high levels. Interestingly, could play critical role optimal function, mediating cell-extrinsic homeostatic effects through regulation danger signaling neoplastic under immunogenic and/or RT. In this review, we summarize preclinical development therapy highlight latest progress nanotechnology augmenting The underlying mechanisms nanomaterial-triggered TME discussed depth. Finally, suggest implications these three combined together achieve goal maximizing advantages show recent advances biomarkers evaluation those therapies.

Language: Английский

Citations

19