Unveiling the antibacterial action of ambroxol against Staphylococcus aureus bacteria: in vitro, in vivo, and in silico investigation DOI Creative Commons
Ahmed Abdelaziz, Amal M. Abo-Kamar,

Alaa E. Ashour

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 29, 2024

Abstract It is critical to find novel therapeutic approaches owing the dissemination of multidrug resistance (MDR) in pathogenic bacteria, particularly Staphylococcus aureus . FDA-drug repurposing an important tactic fight MDR bacteria. Here, we inspected antibacterial activity ambroxol against clinical S. isolates. Using broth microdilution method, revealed minimum inhibitory concentrations (MICs) 0.75 1.5 mg/mL. Also, it antibiofilm action on 42.17% isolates by crystal violet assay. A scanning electron microscope was employed study ambroxol. that association between cells interrupted ambroxol, and biofilm construction devastated. Moreover, qRT-PCR utilized elucidate consequence gene expression efflux biofilm. Remarkably, has downregulated cna , fnb A, ica nor B genes. Ambroxol’s vivo investigated using infected burn infection. Interestingly, improved histological features skin tissues, significantly diminished bacterial burden, increased wound healing percentage. a significant reduction immunohistochemical staining tumor necrosis factor-alpha. Finally, silico investigations were performed potential five possible targets aureus. Ambroxol showed good affinities with CrtM being highest, proposing its probable role mechanisms for ambroxol’s

Language: Английский

Mixed storm in SARS‐CoV‐2 infection: A narrative review and new term in the Covid‐19 era DOI Creative Commons

Basil Mohammed Alomair,

Hayder M. Al‐kuraishy, Ali I. Al‐Gareeb

et al.

Immunity Inflammation and Disease, Journal Year: 2023, Volume and Issue: 11(4)

Published: April 1, 2023

Coronavirus disease 2019 (Covid-19) is caused by a novel severe acute respiratory syndrome coronavirus virus type 2 (SARS-CoV-2) leading to the global pandemic worldwide. Systemic complications in Covid-19 are mainly related direct SARS-CoV-2 cytopathic effects, associated hyperinflammation, hypercytokinemia, and development of cytokine storm (CS). As well, developed due propagation oxidative thrombotic events which may progress state called (TS), respectively. In addition, inflammatory lipid storms also activation cells release bioactive lipids correspondingly. Therefore, present narrative review aimed elucidate interrelated relationship between different types mixed (MS). conclusion, infection induces various including CS, storm, TS storm. These not developing alone since there close them. MS seems be more appropriate than it develops intricate interface reactive oxygen species, proinflammatory cytokines, complement activation, coagulation disorders, activated signaling pathway.

Language: Английский

Citations

11
Unveiling the role of astrogliosis in Alzheimer’s disease Pathology: Insights into mechanisms and therapeutic approaches DOI

Mohit Paidlewar,

Sneha Kumari,

Rishika Dhapola

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 141, P. 112940 - 112940

Published: Aug. 17, 2024

Language: Английский

Citations

4
SARS-CoV-2 infection and dysregulation of nuclear factor erythroid-2-related factor 2 (Nrf2) pathway DOI Creative Commons
Rabab S. Hamad, Hayder M. Al‐kuraishy, Αθανάσιος Αλεξίου

et al.

Cell Stress and Chaperones, Journal Year: 2023, Volume and Issue: 28(6), P. 657 - 673

Published: Oct. 5, 2023

Coronavirus disease 2019 (COVID-19) is a recent pandemic caused by novel severe acute respiratory syndrome coronavirus 2 (SARS‑CoV‑2) leading to pulmonary and extra-pulmonary manifestations due the development of oxidative stress (OS) hyperinflammation. The underlying cause for OS hyperinflammation in COVID-19 may be related inhibition nuclear factor erythroid 2-related (Nrf2), master regulator antioxidative responses cellular homeostasis. Nrf2 pathway inhibits expression pro-inflammatory cytokines cytokine storm COVID-19. activators can attenuate endothelial dysfunction (ED), renin-angiotensin system (RAS) dysregulation, immune thrombosis, coagulopathy. Hence, this review aimed reveal potential role its management As well, we tried revise mechanistic

Language: Английский

Citations

11
Evaluation of the long-term autonomic dysfunction after the recovery of COVID-19 disease DOI Open Access
Ajar Koçak, Sezen Bağlan Uzunget, Kader Eliz Şahin

et al.

Anatolian Current Medical Journal, Journal Year: 2025, Volume and Issue: 7(1), P. 72 - 76

Published: Jan. 10, 2025

Aims: Beyond the acute phase of COVID-19 disease, many patients experience persistent symptoms, collectively termed "post-COVID syndrome," which includes autonomic dysfunction. Heart rate variability (HRV) is a well-established method to assess nervous system (ANS) function. This study aimed investigate long-term impact on function through evaluating changes in HRV. Methods: retrospective included 225 participants divided into two groups: 117 post-COVID and 108 age gender matched controls. HRV was assessed using 24-hour Holter monitoring. Time-domain frequency-domain indices were analyzed, including standard deviation normal-to-normal intervals (SDNN), root mean square successive RR interval differences (RMSSD), low frequency (LF)/high (HF) ratio. Statistical comparisons performed independent t-tests, Mann-Whitney U tests, correlation analyses. Results: Post-COVID exhibited significantly lower compared metrics such as SDNN (135.7±39.5 ms vs 149.1±34.2 ms, p=0.007) RMSSD (32.7±13.7 37.5±14.7 p=0.012) reduced group. Frequency-domain indices, total power (TP) HF power, also diminished. Correlation analysis revealed no significant association between duration time (one-year follow-up) most parameters. Conclusion: dysfunction, marked by parasympathetic activity increased cardiovascular risks, with some evidence partial recovery during sleep. Routine monitoring targeted interventions, alongside further research larger cohorts, are crucial for better understanding effects improving patient outcomes.

Language: Английский

Citations

0
The possible role of nuclear factor erythroid‐2‐related factor 2 activators in the management of Covid‐19 DOI
Hayder M. Al‐kuraishy, Ali I. Al‐Gareeb, Omayma A. Eldahshan

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2023, Volume and Issue: 38(1)

Published: Dec. 9, 2023

COVID-19 is caused by a novel SARS-CoV-2 leading to pulmonary and extra-pulmonary manifestations due oxidative stress (OS) development hyperinflammation. primarily asymptomatic though it may cause acute lung injury (ALI), respiratory distress syndrome (ARDS), systemic inflammation, thrombotic events in severe cases. SARS-CoV-2-induced OS triggers the activation of different signaling pathways, which counterbalances this complication. One these pathways nuclear factor erythroid 2-related 2 (Nrf2), induces series cellular interactions mitigate SARS-CoV-2-mediated viral toxicity OS-induced injury. Nrf2 pathway inhibits expression pro-inflammatory cytokines cytokine storm COVID-19. Therefore, activators play an essential role reducing infection-induced inflammation suppressing NLRP3 inflammasome Furthermore, can attenuate endothelial dysfunction (ED), renin-angiotensin system (RAS) dysregulation, immune thrombosis, coagulopathy. Thus mini-review tries clarify possible management could be effective therapeutic strategy Covid-19. Preclinical clinical studies are recommended regard.

Language: Английский

Citations

9
The Potential Effect of Dapsone on the Inflammatory Reactions in COVID-19: Staggering View DOI

Hayder M Al-Kuraishy,

Ali I. Al‐Gareeb, Engy Elekhnawy

et al.

Combinatorial Chemistry & High Throughput Screening, Journal Year: 2023, Volume and Issue: 27(5), P. 674 - 678

Published: March 31, 2023

Severe SARS-CoV-2 infection is linked with an overstated immune response the succeeding release of pro-inflammatory cytokines and progression cytokine storm. In addition, severe associated development oxidative stress coagulopathy. Dapsone (DPS) a bacteriostatic antibiotic that has potent anti-inflammatory effect. Thus, this mini-review aimed to elucidate potential role DPS in mitigating inflammatory disorders COVID-19 patients. inhibits neutrophil myeloperoxidase, inflammation, chemotaxis. Therefore, could be effective against neutrophilia-induced complications COVID-19. by suppressing expression signaling pathways generation reactive oxygen species (ROS) correspondingly. conclusion, might management through attenuation disorders. preclinical clinical studies are reasonable regard.

Language: Английский

Citations

7
Varenicline solution nasal spray for dry eye disease in Chinese patients: a randomized phase 3 trial DOI
Lei Tian, Xiuming Jin,

Jibing Wang

et al.

The Lancet Regional Health - Western Pacific, Journal Year: 2024, Volume and Issue: 45, P. 101032 - 101032

Published: Feb. 27, 2024

Language: Английский

Citations

2
The Antiviral Activity of Varenicline against Dengue Virus Replication during the Post-Entry Stage DOI Creative Commons

Ching-Lin Lin,

Yan-Tung Kiu,

Ju-Ying Kan

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(10), P. 2754 - 2754

Published: Oct. 11, 2023

Dengue virus (DENV) poses a significant global health challenge, with millions of cases each year. Developing effective antiviral drugs against DENV remains major hurdle. Varenicline is medication used to aid smoking cessation, anti-inflammatory and antioxidant effects. In this study, varenicline was investigated for its potential DENV. This study provides evidence the activity DENV, regardless serotype or cell type used. demonstrated dose-dependent effects in reducing viral protein expression, infectivity, yield Vero A549 cells infected DENV-1 DENV-2, EC50 values ranging from 0.44 1.66 μM. Time-of-addition removal experiments that had stronger inhibitory effect on post-entry stage DENV-2 replication than entry stage, as well preinfection attachment stages. Furthermore, cell-based trans-cleavage assays indicated dose-dependently inhibited proteolytic NS2B-NS3 protease. Docking models revealed formation hydrogen bonds van der Waals forces between specific residues proteases. These results highlight mechanism offering valuable insights further research development treatment infection.

Language: Английский

Citations

4
Nicotine regulates abnormal macrophage polarization and trophoblast invasion associated with preterm labor via the α7nAChR/SIRT1 axis DOI
Aihua Ye, Liling Li,

Haozhong Chen

et al.

Placenta, Journal Year: 2024, Volume and Issue: 147, P. 42 - 51

Published: Jan. 29, 2024

Language: Английский

Citations

1
Potential value of neuroimmunotherapy for COVID-19: efficacies and mechanisms of vagus nerve stimulation, electroacupuncture, and cholinergic drugs DOI Creative Commons
Xianqiang Yu, Qingming Kong

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: July 5, 2023

COVID-19 is an inflammatory disease with multiple organs involved, mainly respiratory symptoms. Although the majority of patients present a mild to moderate self-limited course illness, about 5-10% disorders in severe have life-threatening progression. With exception few drugs that shown outstanding anti-COVID-19 effects, efficacy most remains controversial. An increasing number animal and clinical studies neuromodulation has significant effect on reducing markers COVID-19, thus exerting effective neuroimmunotherapeutic value. Currently, main neuroimmunomodulatory measures against include vagus nerve stimulation, electroacupuncture, cholinergic drugs. In this review, we will summarize research progress potential value neuroimmunotherapy for elaborate its efficacies mechanisms, order provide reliable evidence intervention.

Language: Английский

Citations

1