Identification of novel inflammatory response-related biomarkers in patients with ischemic stroke based on WGCNA and machine learning DOI
Chenyi Huang,

Dengxuan Wu,

Guifen Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Background Ischemic stroke (IS) is one of the most common causes disability in adults worldwide. This study aimed to identify key genes related inflammatory response provide insights into mechanisms and management IS. Methods Transcriptomic data for IS were downloaded from Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) differential expression used inflammation-related (IRGs) associated with Hub IRGs screened using Lasso, SVM-RFE, random forest algorithms, a nomogram diagnostic model was constructed. The performance assessed receiver operating characteristic (ROC) curves calibration plots. Additionally, immune cell infiltration potential small molecule drugs targeting analyzed. Results Nine differentially expressed identified IS, including NMUR1, AHR, CD68, OSM, CDKN1A, RGS1, BTG2, ATP2C1, TLR3. Machine learning algorithms selected three hub (AHR, NMUR1). A based on these showed excellent an area under curve (AUC) greater than 0.9 both training validation sets. Immune revealed higher levels neutrophils activated CD4 + T cells, lower CD8 NK naive B cells patients. exhibited significant correlations infiltration. Furthermore, identified, chrysin, piperine, genistein, resveratrol, which have therapeutic effects Conclusion confirms impact progression provides new targets personalized treatment

Language: Английский

Diet and Nutraceuticals for treatment and prevention of primary and secondary stroke: emphasis on nutritional antiplatelet and antithrombotic agents DOI
Kakarla Ramakrishna,

Lakshmi Anusha Vinjavarapu,

Sairam Krishnamurthy

et al.

Neurochemistry International, Journal Year: 2024, Volume and Issue: 179, P. 105823 - 105823

Published: July 30, 2024

Language: Английский

Citations

5
Intermittent fasting along with hydroalcoholic extract of Centella-asiatica ameliorates sub-acute hypoxia-induced ischemic stroke in adult zebrafish DOI

Priya Bindal,

Kaunava Roy,

Biplob Sarkar

et al.

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 279, P. 109871 - 109871

Published: Feb. 29, 2024

Language: Английский

Citations

4
Identification of novel inflammatory response-related biomarkers in patients with ischemic stroke based on WGCNA and machine learning DOI Creative Commons
Chenyi Huang,

Dengxuan Wu,

Guifen Yang

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 22, 2025

Ischemic stroke (IS) is one of the most common causes disability in adults worldwide. This study aimed to identify key genes related inflammatory response provide insights into mechanisms and management IS. Transcriptomic data for IS were downloaded from Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) differential expression used inflammation-related (IRGs) associated with Hub IRGs screened using Lasso, SVM-RFE, random forest algorithms, a nomogram diagnostic model was constructed. The performance assessed receiver operating characteristic (ROC) curves calibration plots. Additionally, immune cell infiltration potential small molecule drugs targeting analyzed. IRG verified by qRT-PCR healthy controls patients. Nine differentially expressed identified IS, including NMUR1, AHR, CD68, OSM, CDKN1A, RGS1, BTG2, ATP2C1, TLR3. Machine learning algorithms selected three hub (AHR, NMUR1). A based on these showed excellent an area under curve (AUC) greater than 0.9 both training validation sets. Immune revealed higher levels neutrophils activated CD4 + T cells, lower CD8 NK naive B cells exhibited significant correlations infiltration. Furthermore, identified, chrysin, piperine, genistein, resveratrol, which have therapeutic effects evaluation demonstrated that blood biomarkers NMUR1) patients could serve as distinguishing indicators between (P < 0.05). confirmed impact progression provided new targets personalized treatment

Language: Английский

Citations

0
FCGR1A Alleviates Ischemic Stroke-induced Injury by Promoting Anti-Inflammatory Microglial Polarization via the AMPK–mTOR Signaling Pathway DOI Creative Commons
Meng Liu,

Xiang‐Bing Fan,

D Chen

et al.

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(5)

Published: April 29, 2025

Background: Ischemic stroke triggers inflammatory responses that lead to neuronal damage, with microglial polarization significantly influencing post-stroke inflammation. This study explores the role of Fc gamma receptor Ia (FCGR1A) in and its regulatory mechanisms ischemic stroke. Methods: Differentially expressed genes (DEGs) associated were identified using GSE58294 dataset. Hub found by analyzing protein–protein interaction (PPI) networks. BV2 microglia subjected oxygen–glucose deprivation/reoxygenation (OGD/R) mimic conditions vitro, FCGR1A marker levels assessed. Besides, cells stimulated lipopolysaccharide (LPS) interferon-gamma (IFN-γ) induce M1 polarization, effects overexpression knockdown on cytokine production evaluated. The function AMP-activated protein kinase (AMPK)-mTOR pathway regulating was further investigated mTOR inhibitor rapamycin (RAP). Results: From 327 DEGs identified, chosen as a hub gene. OGD/R treatment produced time-dependent rise FCGR1A, induction brown adipocytes 1 (Iba1), interleukin 6 (IL-6) expression, indicating enhanced induced proinflammatory response promoted whereas reduced inflammation shifted toward an anti-inflammatory M2 phenotype. Inhibition RAP, combined knockdown, AMPK activation shift Conclusion: modulates affecting AMPK–mTOR signaling conditions. Targeting related pathways could offer new therapeutic strategies lessen facilitate healing process after

Language: Английский

Citations

0
Long Noncoding RNA ISA1 Protects Against Ischemic Brain Damage by Promoting the Transformation of Microglia Toward Anti-inflammatory Phenotype via the SOCS3/JAK2/STAT3 Pathway DOI

Ermei Lu,

Peng Zhou, Yuanyuan Li

et al.

Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(2)

Published: Feb. 1, 2025

Language: Английский

Citations

0
ncRNA-Mediated Pyroptosis in Cerebral Ischemia-Reperfusion Injury: Pathophysiology, Mechanisms, and Therapeutic Perspectives DOI Creative Commons

Ruiyi Xu,

Peng Quan, Wen Chen

et al.

Current Issues in Molecular Biology, Journal Year: 2025, Volume and Issue: 47(3), P. 141 - 141

Published: Feb. 22, 2025

Cerebral ischemia-reperfusion injury (CIRI) is a complex pathological process triggered by transient obstruction of blood flow and subsequent reperfusion, ultimately leading to intracellular disturbances such as oxidative stress, inflammatory responses, programmed cell death. Among the various types death, pyroptosis (an kind regulated death) has received increasing attention due its involvement in key neurovascular unit cells, including endothelial neurons, microglia, astrocytes. Intriguingly, accumulating evidence demonstrates that non-coding RNAs (ncRNAs), long RNAs, microRNAs, circular can modulate multiple stages CIRI. This review synthesizes recent findings on ncRNAs-regulated We highlight molecular underpinnings pyroptotic activation following ischemic discuss how ncRNAs shape these mechanisms. By elucidating interactions between pyroptosis-related pathways, we intend present innovative viewpoints for early diagnosis development potential therapeutic strategies mitigate

Language: Английский

Citations

0
From oxidative stress to metabolic dysfunction: The role of TRPM2 DOI
Yingshuang Li, Hongwei Ren, Hui Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 284, P. 138081 - 138081

Published: Nov. 26, 2024

Language: Английский

Citations

0
ESTUDOS MULTIDISCIPLINARES SOBRE EMERGÊNCIA E TERAPIA INTENSIVA DOI Open Access

ANTÔNIO JOSÉ MARQUES NOGUEIRA COÊLHO,

BRUNA JULIANE MELO SILVA,

LARISSA NUNES CASTRO

et al.

Published: May 1, 2024

INTRODUÇÃO:O Acidente Vascular Cerebral (AVC) é uma emergência médica causada por obstrução ou ruptura de vasos cerebrais, denominados respectivamente como isquêmico e hemorrágico.Hipertensão tabagismo são fatores risco que aumentam a suscetibilidade ocorrência destes eventos o reconhecimento sinais déficits neurológicos súbitos crucial para um bom prognóstico.A tomografia computadorizada método diagnóstico considerado padrão ouro, tratamento com terapia trombolítica visa reduzir possíveis sequelas

Citations

0
Identification of novel inflammatory response-related biomarkers in patients with ischemic stroke based on WGCNA and machine learning DOI
Chenyi Huang,

Dengxuan Wu,

Guifen Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Background Ischemic stroke (IS) is one of the most common causes disability in adults worldwide. This study aimed to identify key genes related inflammatory response provide insights into mechanisms and management IS. Methods Transcriptomic data for IS were downloaded from Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) differential expression used inflammation-related (IRGs) associated with Hub IRGs screened using Lasso, SVM-RFE, random forest algorithms, a nomogram diagnostic model was constructed. The performance assessed receiver operating characteristic (ROC) curves calibration plots. Additionally, immune cell infiltration potential small molecule drugs targeting analyzed. Results Nine differentially expressed identified IS, including NMUR1, AHR, CD68, OSM, CDKN1A, RGS1, BTG2, ATP2C1, TLR3. Machine learning algorithms selected three hub (AHR, NMUR1). A based on these showed excellent an area under curve (AUC) greater than 0.9 both training validation sets. Immune revealed higher levels neutrophils activated CD4 + T cells, lower CD8 NK naive B cells patients. exhibited significant correlations infiltration. Furthermore, identified, chrysin, piperine, genistein, resveratrol, which have therapeutic effects Conclusion confirms impact progression provides new targets personalized treatment

Language: Английский

Citations

0