Network pharmacology combining molecular docking to unveil the anti-inflammatory mechanism of Aconitum Heterophyllum

Food, Nutrition and Health., Journal Year: 2025, Volume and Issue: 2(1)

Published: March 4, 2025

Abstract Objective The objective of this study was to examine the impact and underlying mechanisms Aconitum Heterophyllum in mitigating cellular inflammation. Methods Initially, principal components A.heterophyllum were obtained from relevant literature sources, corresponding protein targets searched on Pubchem Swiss Target Prediction database. Subsequently, genes linked with inflammation gathered PHARMGKB, OMIM, GeneCards databases. core screened, a protein–protein interaction (PPI) network constructed using Cytoscape 3.9.1. For general targets, gene ontology (GO) analysis Kyoto Encyclopedia Genes Genomes (KEGG) conducted. Molecular docking further applied for compounds. Finally, RAW264.7 model constructed, MTT assay, Griess assay qRT-PCR used initially validate anti-inflammatory effects A. heterophyllum its mechanisms. Results In total, 35 compounds 1322 associated , along 3534 implicated intersection yielded 244 common contributing enrichment 146 pathways. Notably, results molecular revealed strong binding between active targets. Furthermore, vitro experiments indicated potential ’s ingredient through modulation EGFR/JAK2/STAT3 pathway EGFR-AKT pathway, as well inhibiting NO release reduce inflammatory response. Conclusion exerts activity by EGFR-AKT1 signal transduction.

Language: Английский

Probiotics as a Treatment of Chronic Stress Associated Abnormalities

Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown

Published: April 26, 2025

Language: Английский