Analytical Methods,
Journal Year:
2023,
Volume and Issue:
16(1), P. 74 - 82
Published: Nov. 21, 2023
This
study
presents
the
development
of
a
polyester
microplate
for
detecting
S-protein
SARS-CoV-2
virus
in
saliva
and
nasopharyngeal
swab
samples
using
direct
enzyme-linked
immunosorbent
assay
(ELISA)
technology.
The
was
designed
to
contain
96
zones
with
3
mm
diameter
each,
volume
2-3
μL.
experimental
conditions
including
reagent
concentration
reaction
time
were
optimized.
image
digitized
analyzed
graphical
software.
linear
range
obtained
between
protein
S
concentrations
pixel
intensity
0-10
μg
mL
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Feb. 8, 2024
Abstract
Development
of
coating
technologies
for
electrochemical
sensors
that
consistently
exhibit
antifouling
activities
in
diverse
and
complex
biological
environments
over
extended
time
is
vital
effective
medical
devices
diagnostics.
Here,
we
describe
a
micrometer-thick,
porous
nanocomposite
with
both
electroconducting
properties
enhances
the
sensitivity
sensors.
Nozzle
printing
oil-in-water
emulsion
used
to
create
1
micrometer
thick
composed
cross-linked
albumin
interconnected
pores
gold
nanowires.
The
layer
resists
biofouling
maintains
rapid
electron
transfer
kinetics
one
month
when
exposed
directly
fluids,
including
serum
nasopharyngeal
secretions.
Compared
thinner
(nanometer
thick)
made
drop
casting
or
spin
same
thickness,
sensor
exhibits
sensitivities
are
enhanced
by
3.75-
17-fold
three
different
target
biomolecules
tested.
As
result,
emulsion-coated,
multiplexed
can
carry
out
simultaneous
detection
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
nucleic
acid,
antigen,
host
antibody
clinical
specimens
high
specificity.
This
technology
holds
promise
addressing
hurdles
currently
restricting
application
point-of-care
diagnostics,
implantable
devices,
other
healthcare
monitoring
systems.
Biosensors,
Journal Year:
2022,
Volume and Issue:
12(11), P. 927 - 927
Published: Oct. 26, 2022
Electrochemical
biosensors
are
a
family
of
that
use
an
electrochemical
transducer
to
perform
their
functions.
In
recent
decades,
many
have
been
created
for
pathogen
detection.
These
detecting
infections
comprehensively
studied
in
terms
transduction
elements,
biorecognition
components,
and
methods.
This
review
discusses
the
components
may
be
used
identify
pathogens.
include
antibodies
aptamers.
The
integration
transducers
electrode
changes
biosensor
design
is
major
discussion
topic.
Pathogen
detection
methods
can
categorized
by
sample
preparation
secondary
binding
processes.
Diagnostics
medicine,
environmental
monitoring,
biothreat
benefit
from
ensure
food
water
safety.
Disposable
reusable
process
as
well
multiplexed
conformal
detection,
all
included
this
review.
It
now
possible
wide
range
diseases
using
applied
food,
bodily
fluids,
even
objects’
surfaces.
sensitivity
optical
techniques
superior
approaches,
but
prohibitively
expensive
challenging
most
end
users
utilize.
On
other
hand,
approaches
simpler
use,
efficacy
identifying
still
far
satisfactory.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(15), P. 8155 - 8155
Published: July 26, 2024
At
present,
COVID-19
remains
a
public
health
concern
due
to
the
ongoing
evolution
of
SARS-CoV-2
and
its
prevalence
in
particular
countries.
This
paper
provides
an
updated
overview
epidemiology
pathogenesis
COVID-19,
with
focus
on
emergence
variants
phenomenon
known
as
‘long
COVID’.
Meanwhile,
diagnostic
detection
advances
will
be
mentioned.
Though
many
inventions
have
been
made
combat
pandemic,
some
outstanding
ones
include
multiplex
RT-PCR,
which
can
used
for
accurate
diagnosis
infection.
ELISA-based
antigen
tests
also
appear
potential
tools
available
future.
discusses
current
treatments,
vaccination
strategies,
well
emerging
cell-based
therapies
The
underscores
necessity
us
continuously
update
scientific
understanding
treatments
it.
ACS Applied Nano Materials,
Journal Year:
2024,
Volume and Issue:
7(8), P. 9136 - 9146
Published: April 15, 2024
We
report
a
silver
nanoparticle
(AgNP)
sensor
array
to
detect
SARS-CoV-2
viral
particles
utilizing
five
peptide
receptors
produce
colorimetric
response.
show
that
the
interaction
of
virus
with
peptides
interferes
shift
in
plasmonic
absorbance
and
hinders
formation
snowflake-like
fractal
assemblies
between
AgNPs
bridging
peptides.
The
limit
detection
water
was
500,000
copies/mL.
demonstrate
capabilities
distinguish
(Beta,
Delta,
Omicron
variants)
from
influenza
at
99%
confidence
level
through
linear
discriminant
analysis.
also
able
discriminate
three
out
negative
exhaled
breath
condensate
samples
positive
EBC
samples.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(2), P. 67 - 67
Published: Jan. 22, 2025
The
coronavirus
disease
(COVID-19)
pandemic
has
created
an
urgent
need
for
rapid,
accurate,
and
cost-effective
diagnostic
tools.
In
this
study,
economical
electrochemical
immunosensor
the
rapid
diagnosis
of
COVID-19
was
developed
optimized
based
on
charge
transfer
resistance
(Rct)
values
obtained
by
impedance
spectroscopy
(EIS)
from
interaction
between
antibodies
(anti-SARS-CoV-2)
immobilized
as
a
bioreceptor
virus
(SARS-CoV-2).
sensor
uses
modified
pencil
graphite
electrodes
(PGE)
coated
with
poly(4-hydroxybenzoic
acid),
anti-SARS-CoV-2,
silver
nanoparticles.
immobilization
anti-SARS-CoV-2
at
concentration
1:250
30
min,
followed
blocking
surface
0.01%
bovine
serum
albumin
10
min.
optimal
conditions
detection
in
clinical
samples
were
1:10
dilution
response
time
20
responded
linearly
range
0.2–2.5
×
106
particles/μL.
From
relationship
signal
analyzed
sample,
limit
(LOD)
quantification
(LOQ)
1.21
4.04
particles/μL,
respectively.
device
did
not
cross-react
other
viruses,
including
Influenza
A
B,
HIV,
Vaccinia
virus.
relative
standard
deviation
(RSD)
six
immunosensors
prepared
using
shared-pool
sample
3.87.
Decreases
22.3%
12.4%
observed
ten
stored
25
°C
4.0
°C,
provides
timely
accurate
results
high
sensitivity
specificity,
offering
alternative
to
existing
methods.
