Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations DOI Open Access
Joanna Karczewska-Golec, Kamila Sadowska, Piotr Golec

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11222 - 11222

Published: Oct. 18, 2024

For years, gold nanoparticles (AuNPs) have been widely used in medicine and industry. Although various experimental procedures reported for their preparation manipulation, none of them is optimal all purposes. In this work, we engineered the N-terminus pIII minor coat protein bacteriophage (phage) M13 to expose a novel HLYLNTASTHLG peptide that effectively specifically binds gold. addition binding gold, phage could synthesize spherical AuNPs 20 nm other sizes depending on reaction conditions, aggregate them, precipitate from colloid, as revealed by transmission electron microscopy (TEM), atomic force (AFM), scanning (SEM), well ultraviolet–visible (UV–vis) Fourier-transform infrared (FTIR) spectroscopic methods. We demonstrated exposing foreign selected phage-displayed library may serve sustainable molecular factory both synthesis subsequent overnight ions at room temperature neutral pH absence strong reducing agents, such commonly NaBH4. Taken together, results suggest potential applicability new, vitro-identified gold-binding diverse biomimetic manipulations.

Language: Английский

Plasmonic nanoparticle sensors: current progress, challenges, and future prospects DOI Creative Commons
Krishna Kant, Reshma Beeram, Yi Cao

et al.

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(12), P. 2085 - 2166

Published: Jan. 1, 2024

This comprehensive review summarizes the past, present, and future of plasmonic NP-based sensors in terms different sensing platforms, chemical biological analytes, expected technologies.

Language: Английский

Citations

18

Aptamers and antibodies in optical biosensing DOI Creative Commons
Kelvin Mpofu, Sipho H. Chauke, Lungile Nomcebo Thwala

et al.

Discover Chemistry., Journal Year: 2025, Volume and Issue: 2(1)

Published: Feb. 24, 2025

Language: Английский

Citations

1

Nano biosensors: Classification, electrochemistry, nanostructures, and optical properties DOI Creative Commons
Ahmed Mahdi Rheima, Zainab T. Al‐Sharify, Ameen Alwan Mohaimeed

et al.

Results in Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 103428 - 103428

Published: Nov. 1, 2024

Language: Английский

Citations

5

Gold Nanoprobes for Robust Colorimetric Detection of Nucleic Acid Sequences Related to Disease Diagnostics DOI Creative Commons
Maria Enea, Andreia Leite, Ricardo Franco

et al.

Nanomaterials, Journal Year: 2024, Volume and Issue: 14(22), P. 1833 - 1833

Published: Nov. 16, 2024

Gold nanoparticles (AuNPs) are highly attractive for applications in the field of biosensing, particularly colorimetric nucleic acid detection. Their unique optical properties, which sensitive to changes their environment, make them ideal candidates developing simple, rapid, and cost-effective assays. When functionalized with oligonucleotides (Au-nanoprobes), they can undergo aggregation or dispersion presence complementary sequences, leading distinct color that serve as a visual signal Aggregation-based assays offer significant advantages over other homogeneous assays, such fluorescence-based methods, namely, label-free protocols, rapid interactions solutions, detection by naked eye using low-cost instruments. Despite promising results, application Au-nanoprobe-based complex biological matrices faces several challenges. The most related colloidal stability oligonucleotide functionalization Au-nanoprobes but also mode type method, spacer, oligo-AuNPs ratio, pH, temperature, ionic strength influence Au-nanoprobe thus performance assay. This review elucidates characteristics determined gold (AuNPs)-based detection, how sensitivity specificity These assay fundamental low-cost, robust biomedical sensors perform effectively fluids.

Language: Английский

Citations

1

Engineered M13-Derived Bacteriophages Capable of Gold Nanoparticle Synthesis and Nanogold Manipulations DOI Open Access
Joanna Karczewska-Golec, Kamila Sadowska, Piotr Golec

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11222 - 11222

Published: Oct. 18, 2024

For years, gold nanoparticles (AuNPs) have been widely used in medicine and industry. Although various experimental procedures reported for their preparation manipulation, none of them is optimal all purposes. In this work, we engineered the N-terminus pIII minor coat protein bacteriophage (phage) M13 to expose a novel HLYLNTASTHLG peptide that effectively specifically binds gold. addition binding gold, phage could synthesize spherical AuNPs 20 nm other sizes depending on reaction conditions, aggregate them, precipitate from colloid, as revealed by transmission electron microscopy (TEM), atomic force (AFM), scanning (SEM), well ultraviolet–visible (UV–vis) Fourier-transform infrared (FTIR) spectroscopic methods. We demonstrated exposing foreign selected phage-displayed library may serve sustainable molecular factory both synthesis subsequent overnight ions at room temperature neutral pH absence strong reducing agents, such commonly NaBH4. Taken together, results suggest potential applicability new, vitro-identified gold-binding diverse biomimetic manipulations.

Language: Английский

Citations

0