Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(22), P. 8949 - 8955
Published: May 21, 2024
Here,
we
demonstrate
the
detection
of
nanoplastics
(NPLs)
in
flow
with
stimulated
Raman
scattering
(SRS)
for
first
time.
NPLs
(plastic
particles
<1000
nm)
have
recently
been
detected
different
environmental
samples
and
personal
care
products.
However,
their
characterization
is
still
an
analytical
challenge.
Multiple
parameters,
including
size,
chemical
composition,
concentration
(particle
number
mass),
need
to
be
determined.
In
earlier
paper,
online
field
fractionation
(FFF)-Raman
analysis
optical
trapping
was
shown
a
promising
tool
this
size
range.
SRS,
which
based
on
enhancement
vibrational
transition
by
matching
energy
difference
two
laser
beams,
would
allow
much
more
sensitive
and,
hence,
shorter
acquisition
times
compared
spontaneous
microspectroscopy
(RM).
show
applicability
SRS
flow-based
individual,
untrapped
NPLs.
It
possible
detect
polyethylene
(PE),
polystyrene
(PS),
poly(methyl
methacrylate)
(PMMA)
beads
diameters
100-5000
nm.
The
high
time
resolution
60.5
μs
allows
us
individual
signals
per
particle
correlate
injected
mass
concentration.
Furthermore,
due
resolution,
optically
trapped
could
distinguished
from
peak
shapes.
wavenumber
settings
add
selectivity
measurement.
Whereas
necessary
RM,
current
study
demonstrates
that
can
without
trapping.
Additionally,
mean
estimated
using
width
(duration)
intensity
signals.
Pharmaceutics,
Journal Year:
2024,
Volume and Issue:
16(6), P. 709 - 709
Published: May 24, 2024
Of
all
the
numerous
nanosized
extracellular
vesicles
released
by
a
cell,
endosomal-originated
exosomes
are
increasingly
recognized
as
potential
therapeutics,
owing
to
their
inherent
stability,
low
immunogenicity,
and
targeted
delivery
capabilities.
This
review
critically
evaluates
transformative
of
exosome-based
modalities
across
pharmaceutical
precision
medicine
landscapes.
Because
precise
biomolecular
cargo
delivery,
posited
ideal
candidates
in
drug
enhancing
regenerative
strategies,
advancing
diagnostic
technologies.
Despite
significant
market
growth
projections
exosome
therapy,
its
utilization
is
encumbered
substantial
scientific
regulatory
challenges.
These
include
lack
universally
accepted
protocols
for
isolation
complexities
associated
with
navigating
environment,
particularly
guidelines
set
forth
U.S.
Food
Drug
Administration
(FDA).
presents
comprehensive
overview
current
research
trajectories
aimed
at
addressing
these
impediments
discusses
prospective
advancements
that
could
substantiate
clinical
translation
exosomal
therapies.
By
providing
analysis
both
capabilities
hurdles
therapeutic
applications,
this
article
aims
inform
direct
future
paradigms,
thereby
fostering
integration
systems
into
mainstream
practice.
Scientific Reports,
Journal Year:
2023,
Volume and Issue:
13(1)
Published: Sept. 22, 2023
Abstract
We
present
a
generically
applicable
approach
to
determine
an
extensive
set
of
size-dependent
critical
quality
attributes
inside
nanoparticulate
pharmaceutical
products.
By
coupling
asymmetrical-flow
field-flow
fractionation
(AF4)
measurements
directly
in-line
with
solution
small
angle
X-ray
scattering
(SAXS),
vital
information
such
as
(i)
quantitative,
absolute
size
distribution
profiles,
(ii)
drug
loading,
(iii)
internal
structures,
and
(iv)
quantitative
on
free
is
obtained.
Here
the
validity
method
was
demonstrated
by
characterizing
complex
mRNA-based
lipid
nanoparticle
The
particularly
particles
in
range
100
nm
below,
which
highly
relevant
for
products—both
biologics
nanoparticles.
can
be
applied
well
other
fields,
including
structural
biology
environmental
sciences.
Environmental Science and Ecotechnology,
Journal Year:
2024,
Volume and Issue:
21, P. 100428 - 100428
Published: May 3, 2024
Micro/nanoplastics
(MNPs)
are
detected
in
human
liver,
and
pose
significant
risks
to
health.
Oral
exposure
MNPs
derived
from
non-biodegradable
plastics
can
induce
toxicity
mouse
liver.
Similarly,
nasal
cause
airway
dysbiosis
mice.
However,
the
hepatotoxicity
induced
by
foodborne
airborne
biodegradable
remains
poorly
understood.
Here
we
show
hepatotoxic
effects
of
polylactic
acid
(PLA)
through
multi-omics
analysis
various
biological
samples
mice,
including
gut,
fecal,
nasal,
lung,
blood
samples.
Our
results
that
both
PLA
compromise
liver
function,
disrupt
serum
antioxidant
activity,
pathology.
Specifically,
lead
gut
microbial
dysbiosis,
metabolic
alterations
serum,
transcriptomic
changes.
Airborne
affect
lung
microbiota,
alter
metabolites,
transcriptomics.
The
Lachnospiraceae_NK4A136_group
is
a
potential
biomarker
for
MNP
exposure,
while
unclassified
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(22), P. 8846 - 8854
Published: May 17, 2024
Despite
growing
ecological
concerns,
studies
on
microplastics
and
nanoplastics
are
still
in
their
initial
stages
owing
to
technical
hurdles
analytical
techniques,
especially
for
nanoplastics.
We
provide
an
overview
of
the
general
attributes
micro/nanoplastics
natural
environments
techniques
commonly
used
analysis.
After
demonstrating
challenges
associated
with
identification
due
distinctive
characteristics,
we
discuss
recent
technological
advancements
detecting
EFSA Supporting Publications,
Journal Year:
2024,
Volume and Issue:
21(9)
Published: Sept. 1, 2024
Abstract
New
Approach
Methodologies
(NAMs),
broadly
understood
to
include
in
silico,
chemico,
vitro
and
ex
vivo
methods,
show
great
potential
advancing
risk
assessment
albeit
their
regulatory
implementation
is
lagging.
The
EFSA
Guidance
on
of
nanomaterials
(EFSA
Nano‐RA)
suggests
nano‐specific
best
achieved
through
Integrated
Approaches
Testing
Assessment
(IATAs)
with
NAMs
as
the
first
choice
generate
new
information.
Integrating
promises
several
advantages
such
a
better
human
focus,
more
detailed
insights
into
molecular
mechanisms
higher
efficacy.
However,
applying
NMs
also
poses
considerable
challenges
issues
related
dispersion
stability,
dosimetry,
agglomeration,
dissolution,
transformations
or
assay
interferences.
Significant
efforts
are
being
undertaken
by
standardisation
organisations
research
projects
establish
various
for
NMs.
Here
thorough
review
provided
covering
that
will
be
potentially
useful
food
feed
sector.
It
follows
structure
Nano‐RA
expands
it,
where
needed,
support
decision‐making
selection
NM
assessment.
begins
an
overview
NAM‐frameworks,
followed
description
individual
including
those
relevant
physicochemical
characterisation,
exposure
hazard
toxicodynamics
toxicokinetics.
focus
concerning
degradation/dissolution,
genotoxicity,
cytotoxicity,
oxidative
stress,
(pro‐)inflammation,
barrier
integrity
important
endpoints
initial
screening
according
framework.
As
result,
total
267
nano‐relevant
NAMs,
mostly
“not
validated”
(with
few
notable
exceptions),
were
included
this
review.
Validation
notwithstanding,
could
already
prove
reliable
NMs,
especially
integrated
approaches.
Technologies,
Journal Year:
2025,
Volume and Issue:
13(3), P. 94 - 94
Published: March 1, 2025
This
paper
presents
a
comprehensive
review
of
quality
control
(QC)
and
reliability
research
in
micro–nano
technology,
which
is
vital
for
advancing
microelectronics,
biomedical
engineering,
manufacturing.
Micro-
nanotechnologies
operate
at
different
scales,
yet
both
require
precise
to
ensure
the
performance
durability
small-scale
systems.
synthesizes
key
methodologies,
including
statistical
methods,
machine
learning
AI-driven
advanced
techniques
emphasizing
their
relevance
nanotechnology
applications.
The
also
discusses
application
micro/nanotechnology
other
technological
areas.
discussion
extends
unique
challenges
posed
by
systems,
such
as
failure
modes
related
stiction,
material
fatigue,
environmental
factors.
Advanced
testing
modeling
approaches
are
highlighted
effectiveness
predicting
mitigating
risks.
Additionally,
explores
integration
emerging
technologies
enhance
improve
By
examining
established
novel
techniques,
this
underscores
evolving
nature
field.
It
identifies
areas
future
investigation,
particularly
adaptation
these
methods
increasing
complexity
concludes
proposing
directions
that
can
further
optimize
continued
advancement
industrial
technologies.
