Integrating Ultra‐High‐Performance Liquid Chromatography and Orbitrap High‐Resolution Mass Spectrometry, Feature‐Based Molecular Networking, and Network Medicine to Unlock Harvesting Strategies for Endangered Sinocalycanthus Chinensis

Journal of Separation Science, Journal Year: 2025, Volume and Issue: 48(1)

Published: Jan. 1, 2025

Evaluating the practical utility of endangered plant species is crucial for their conservation. Nevertheless, numerous plants, including Sinocalycanthus chinensis, lack historical usage data, leading to a paucity guidance in traditional pharmacological research. This gap impedes development and potential utilization. Ultra-high-performance liquid chromatography Orbitrap high-resolution mass spectrometry were employed analyze S. chinensis leaves collected at different harvesting times. Then, metabolites automatically annotated by self-built R script conjunction with characteristic fragment ions, neutral loss filtering, feature-based molecular networking. By integrating metabolomics network medicine analysis, optimal harvest times unlocked. A total 305 identified, 66.8% script. progressive increase metabolite disparities was observed from May August, followed relatively minor distinction August October. Notably diverse detected harvested during periods, implying variations efficacy. Network analysis indicated possible therapeutic implications lung cancer, diabetes, bladder Alzheimer's disease. Samples September demonstrated exceptional Harvesting strategically conducted these months based on sample characteristics content, tailored intended applications dietary or medicinal purposes. study developed an efficient methodology investigating exploring food herbal medicine. Consequently, it provides technical support sustainable conservation plants limited clinical application experience.

Language: Английский

Advances in Chromatographic and Mass Spectrometric Techniques for Analyzing Reducing Monosaccharides and Their Phosphates in Biological Samples

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 23

Published: June 10, 2024

Reducing monosaccharides and their phosphates are critical metabolites in the central carbon metabolism pathway of living organisms. Variations content can indicate abnormalities metabolic pathways onset certain diseases, necessitating analysis detection. exhibit significant variations within biological samples present many isomers, which makes accurate quantification reducing a challenging task. Various analytical methods such as spectroscopy, fluorescence detection, colorimetry, nuclear magnetic resonance sensor-based techniques, chromatography, mass spectrometry employed to detect phosphates. In comparison, chromatography highly favored for ability simultaneously analyze multiple components high sensitivity selectivity. This review thoroughly evaluates current chromatographic spectrometric used detecting from 2013 2023, highlighting efficacy advancements these technologies.

Language: Английский

Determination of double bond positions in unsaturated fatty acids by pre-column derivatization with dimethyl and dipyridyl disulfide followed by LC-SWATH-MS analysis

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 5, 2024

Language: Английский