Journal of Mathematical and Fundamental Sciences,
Journal Year:
2022,
Volume and Issue:
54(1), P. 190 - 210
Published: Oct. 18, 2022
Blister
beetles
are
an
important
object
of
study
in
the
field
agriculture
and
health.
Despite
being
known
to
be
for
host
animal,
gut
bacteria
blister
have
not
been
investigated
deeply.
This
aimed
provide
first
initial
description
bacterial
community
Mylabris
pustulata
as
one
species
beetles,
based
on
a
culture-independent
technique.
Adult
same
colony
were
sampled
confirmed
Thunberg.
The
content
was
used
high
throughput
sequencing,
targeting
V3-V4
regions
16S
rRNA
gene,
cultivation
resident
bacteria.
results
showed
that
higher
richness
present
communities
when
compared
grown
culture.
Proteobacteria
most
abundant
phylum
M.
pustulata,
whereby
reads
belonged
class
Gammaproteobacteria.
dominant
genera
determined
Enterobacter,
Acinetobacter,
Enterococcus,
Klebsiella,
Pseudomonas.
In
addition,
our
attempts
led
successful
isolation
members
Klebsiella
Enterococcus.
subject
this
could
explored
further
find
potential
roles
these
microbiota
specific
their
bioprospects.
Integrative Zoology,
Journal Year:
2024,
Volume and Issue:
19(6), P. 1092 - 1104
Published: Feb. 20, 2024
Herbicides
have
demonstrated
their
impact
on
insect
fitness
by
affecting
associated
microbiota
or
altering
the
virulence
of
entomopathogenic
fungi
toward
insects.
However,
limited
research
has
explored
implications
herbicide
stress
intricate
tripartite
interaction
among
insects,
bacterial
communities,
and
entomopathogens.
In
this
study,
we
initially
that
bacteria
confer
a
leaf
beetle,
Plagiodera
versicolora,
with
capability
to
resist
fungus
Aspergillus
nomius
infection,
sustained
even
under
glyphosate
stress.
Further
analysis
revealed
significant
alteration
in
abundance
composition
due
treatment.
The
dominant
bacterium,
post
A.
infection
following
combination
treatments,
exhibited
strong
suppressive
effects
fungal
growth.
Additionally,
markedly
inhibited
pathogenic
bacterium
Pseudomonas
though
it
P.
versicolora's
immunity,
ultimately
enhancing
beetle's
tolerance
nomius.
summary,
our
findings
suggest
bestow
an
augmented
resilience
against
dual
stressors
both
entomopathogen
glyphosate.
These
results
provide
insight
into
residues
interactions
bacteria,
fungi,
holding
for
pest
control
ecosystem
assessment.
Science,
Journal Year:
2024,
Volume and Issue:
384(6693)
Published: April 18, 2024
The
commensal
microbiota
of
the
mosquito
gut
plays
a
complex
role
in
determining
vector
competence
for
arboviruses.
In
this
study,
we
identified
bacterium
from
field
Aedes
albopictus
mosquitoes
named
Rosenbergiella
sp.
YN46
(
Rosenbergiella_
YN46)
that
rendered
refractory
to
infection
with
dengue
and
Zika
viruses.
Inoculation
1.6
×
10
3
colony
forming
units
(CFUs)
into
A.
effectively
prevents
viral
infection.
Mechanistically,
secretes
glucose
dehydrogenase
Ry
GDH),
which
acidifies
lumen
fed
mosquitoes,
causing
irreversible
conformational
changes
flavivirus
envelope
protein
prevent
entry
cells.
semifield
conditions,
exhibits
effective
transstadial
transmission
blocks
virus
by
newly
emerged
adult
mosquitoes.
prevalence
is
greater
low-dengue
areas
(52.9
~91.7%)
than
those
dengue-endemic
regions
(0
~6.7%).
may
offer
an
safe
lead
biocontrol.
Integrative Zoology,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 26, 2024
Agricultural
ecosystem
formation
and
evolution
depend
on
interactions
communication
between
multiple
organisms.
Within
this
context,
occurs
microbes,
plants,
insects,
often
involving
the
release
perception
of
a
wide
range
chemical
cues.
Unraveling
how
information
is
coded
interpreted
critical
to
expanding
our
understanding
agricultural
ecosystems
function
in
terms
competition
cooperation.
Investigations
examining
dual
(e.g.
plant-microbe,
insect-microbe,
insect-plant)
have
resolved
some
basic
components
communication.
However,
there
need
for
systematically
multitrophic
that
occur
simultaneously
microorganisms,
plants.
A
more
thorough
these
has
been
made
possible
by
recent
advancements
study
such
ecological
interactions,
which
are
based
variety
contemporary
technologies
as
artificial
intelligence
sensors,
multi-omics,
metabarcoding,
others.
Frequently,
developments
led
discovery
startling
examples
each
member
manipulating
other.
Here,
we
review
advances
bottom-up
their
consequences.
We
discuss
"chemo-languages"
they
modify
outcomes
multi-species
across
trophic
levels.
Further,
suggest
prospects
translating
current
into
strategies
could
be
applied
increase
food
safety
security.
International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(3), P. 2434 - 2434
Published: Jan. 26, 2023
Nutritional
symbionts
of
sap-sucking
auchenorrhynchan
insects
Hemiptera
are
usually
confined
to
the
bacteriomes
and/or
fat
bodies.
Knowledge
is
limited
about
distribution
microbial
in
other
organs.
We
investigated
obligate
salivary
glands,
gut
tissues,
reproductive
organs,
bacteriomes,
and
bodies
two
cicada
species,
Karenia
caelatata
Tanna
sp.,
using
integrated
methods,
including
a
modified
fluorescence
situ
hybridization
(FISH)
technique,
which
can
greatly
enhance
FISH
signal
intensity
related
symbionts.
revealed
that
Candidatus
Sulcia
muelleri
(Sulcia)
yeast-like
fungal
symbiont
(YLS)
were
harbored
bodies,
respectively.
Both
YLS
be
transmitted
offspring
via
ovaries,
forming
"symbiont
ball"
each
egg.
Neither
nor
tissues
testes.
Phylogenetic
trees
both
cicadas
confirm
K.
member
tribe
Dundubiini,
Leptopsaltriini
comprises
Ta.
sp.
not
monophyletic.
embedded
inside
lineage
whereas
closely
clade
comprising
cicada-parasitizing
fungi
Ophiocordyceps
Mogannia
conica
Meimuna
mongolica,
suggesting
an
evolutionary
replacement
from
fungus
another
fungus.
Our
results
provide
new
insights
into
symbiosis
between
Cicadidae
Modification
through
addition
helpers
heat
shock
enhanced
YLS,
may
guidelines
for
enhancement
symbiont(s)
experiments.
Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
12(5)
Published: March 18, 2024
ABSTRACT
In
this
study,
we
first
time
sequenced
and
analyzed
the
16S
rRNA
gene
data
of
predator
ladybird
beetles
Novius
pumilus
globally
distributed
invasive
pest
Icerya
aegyptiaca
at
different
stages,
combined
with
bacterial
genome
sequences
in
N.
to
explored
taxonomic
distribution,
alpha
beta
diversity,
differentially
abundant
bacteria,
co-occurrence
network,
putative
functions
their
microbial
community.
Our
finding
revealed
that
Candidatus
Walczuchella,
which
exhibited
a
higher
abundance
I.
,
possessed
several
genes
essential
amino
acid
biosynthesis
seemed
perform
roles
providing
nutrients
host,
similar
other
obligate
symbionts
scale
insects.
Lactococcus
Serratia
Pseudomonas
more
were
predicted
have
related
hydrocarbon,
fatty
acids,
chitin
degradation,
may
assist
hosts
digesting
wax
shell
covering
Notably,
our
result
showed
had
relatively
abundances
adults
eggs
compared
stages
indicating
potential
vertical
transmission.
Additionally,
found
Arsenophonus
known
influence
sex
ratios
whitefly
wasp,
also
function
probably
by
influencing
nutrient
metabolism
as
it
similarly
many
corresponding
vitamin
B
biosynthesis.
Also,
observed
horizontal
transfer
between
insect
its
predator,
high
ladybirds
bacteria
from
IMPORTANCE
The
composition
dynamic
changes
microbiome
developmental
prey
detected.
We
aegyptiaca,
provide
host
based
on
biosynthesis-related
genes.
Abundant
including
Pseudophonus
help
digest
insects
acid,
degrading-related
A
key
endosymbiont
play
metabolisms
determination
is
possibly
transferred
predator.
Insects,
Journal Year:
2024,
Volume and Issue:
15(3), P. 181 - 181
Published: March 7, 2024
The
blowfly,
Aldrichina
grahami
(Diptera:
Calliphoridae),
is
a
well-known
forensically
important
insect.
Basic
data
related
to
A.
have
been
well
documented;
but
despite
the
pivotal
role
of
gut
microbes
in
various
facets
insect
biology,
little
known
about
its
microbiome.
To
investigate
bacterial
community
and
explore
stability,
diet
developmental
stage
were
selected
as
two
variables.
Larvae
reared
on
bovine
liver,
swine
manure,
chicken
high-throughput
sequencing
1st,
2nd,
3rd
instar
larvae
newly
emerged
adults
was
performed.
According
our
results,
alpha
diversity
did
not
significantly
vary
among
different
diets
stages.
Principal
coordinate
analysis
revealed
that
microbiome
clustered
together
main
phyla
microbial
Firmicutes
Proteobacteria,
dominant
genera
Vagococcus,
Providencia,
Lactobacillus,
Morganella.
These
findings
characterized
demonstrated
fairly
stable.
Morganella
potential
serve
core
microbiomes
within
grahami.
Microbiology Spectrum,
Journal Year:
2022,
Volume and Issue:
10(5)
Published: Aug. 22, 2022
Fast-growing
bamboo
may
be
a
source
of
high-quality
cellulose
with
the
potential
to
contribute
energy
sustainability,
if
an
efficient
and
low-cost
solution
decomposition
can
developed.
This
study
compared
gut
microbiomes
rhinoceros
beetle
(Trypoxylus
dichotomus)
feeding
on
wood
fiber.
The
results
revealed
that
diet
has
distinctive
effect
microbial
composition
in
midgut,
including
its
most
abundant
microorganisms
fermentation
chemoheterotroph
pathways.
After
identifying
13
bacterial
isolates,
we
constructed
natural
system
based
relative
abundance
artificial
equal
proportions
each
isolate
catabolize
lignocellulose.
Enterobacter
sp.
AZA_4_5
showed
higher
degradation
efficiency
than
other
single
strains
or
system.
thus
serve
as
important
reference
for
further
research
development
synthetic
consortium
maximize
lignocellulolytic
ability.
IMPORTANCE
Bamboo
produces
great
yield
lignocellulosic
biomass
due
high
carbon
fixing.
microbiome
Trypoxylus
dichotomus
differed
between
fiber
diets.
pathways
were
enriched
bacteria
diet.
highly
isolates
identified
from
whereas
well
one
indicate
could
provide
effective
utilize
biomass.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2023,
Volume and Issue:
13
Published: July 6, 2023
The
red
imported
fire
ants
(RIFA,
Solenopsis
invicta)
have
become
a
well-known
invasive
species
that
poses
significant
ecological
and
economic
threats
globally.
As
of
recent
times,
the
geographic
scope
its
invasion
in
China
is
rapidly
expanding,
thereby
aggravating
extent
severity
detrimental
effects.
importance
soil
microorganisms
for
maintaining
health
ecosystem
function
has
been
widely
acknowledged.
However,
negative
impact
RIFAs
on
microbial
communities
their
functions
not
yet
fully
understood.
In
this
study,
we
sequenced
V3-V4
variable
region
bacterial
16S
rRNA
gene
samples
collected
from
three
types
RIFA
nests
to
investigate
diversity
composition.
results
alpha
analysis
showed
normal
without
exhibited
highest
level
diversity,
followed
by
RIFA-invaded
abandoned
nests.
Taxonomy
biological
annotation
analyses
revealed
differences
community
structure
among
different
samples.
Our
findings
demonstrate
can
significantly
alter
composition,
which
could
ultimately
affect
function.
Therefore,
effective
management
strategies
are
urgently
needed
mitigate
native
ecosystems.
