Essential Roles of Two FRQ Proteins (Frq1 and Frq2) in Beauveria bassiana’s Virulence, Infection Cycle, and Calcofluor-Specific Signaling

Applied and Environmental Microbiology, Journal Year: 2021, Volume and Issue: 87(6)

Published: Jan. 5, 2021

Two FRQ proteins (Frq1 and Frq2) distinct in molecular mass structure coexist Beauveria bassiana, an asexual insect-pathogenic fungus. Frq1 Frq2 have been proven to opposite nuclear rhythms that can persistently activate developmental activator genes hence orchestrate nonrhythmic conidiation vitro under light or darkness. Here, we report the essentiality of either FRQ, but being more important than Frq1, for fungal virulence infection cycle. The was attenuated significantly absence frq2 frq1 through normal cuticle cuticle-bypassing by intrahemocoel injection, accompanied differentially reduced secretion Pr1 proteases required delayed development hyphal bodies vivo, which usually propagate yeast-like budding host hemocoel accelerate insect death from mycosis. Despite insignificant changes radial growth normal, oxidative, hyperosmotic culture conditions, conidial yields Δfrq1 Δfrq2 mutants on cadavers were sharply reduced, reduction increased with shortening daylight length day 9 12 after death, indicating both are cycle habitats. Intriguingly, showed hypersensitivity high resistance cell wall-perturbing calcofluor white, coinciding respectively calcofluor-triggered cells' hypo- hyperphosphorylated signals Slt2, a mitogen-activated protein kinase (MAPK) mediation wall integrity. This finding offers novel insight into roles calcofluor-specific signal transduction via Slt2 cascade.IMPORTANCE Opposite two coexisting pathogen shown circadian independent photoperiod change. paper reports essential Frq2, greater role sustaining since deletion led marked delay lethal action against model drastic yield cadavers. Moreover, display perturbation MAPK/Slt2 white-triggered cells, respectively. These findings uncover requirement habitats provide their cascade.

Language: Английский

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Essential Role of WetA, but No Role of VosA, in Asexual Development, Conidial Maturation and Insect Pathogenicity of Metarhizium robertsii

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 14, 2023

Conidial maturation, which is crucial for conidial quality, controlled by the asexual development activator WetA and downstream, velvety protein VosA in Aspergillus. Their orthologs have proved functional quality control of Beauveria bassiana, as seen Aspergillus, but are functionally unexplored, Metarhizium robertsii, another hypocrealean insect pathogen. Here, prove essential nonessential M. robertsii's life cycle, respectively. Disruption wetA increased hyphal sensitivity to oxidative stress Congo red-induced cell wall stress, had little impact on radial growth. The ΔwetA mutant was severely compromised conidiation capacity featured slower germination, decreased UV resistance, reduced hydrophobicity, deformed hydrophobin rodlet bundles that were assembled onto coat. mutant's virulence greatly attenuated via normal infection due a blockage infection-required cellular processes. All examined phenotypes unaffected ΔvosA mutant. Intriguingly, mannitol much less accumulated 7- 15-day-old cultures than strains, while trehalose not detectable at all, revealing link intracellular polyol accumulation maturation. Transcriptomic analysis revealed differential regulation 160 genes (up/down ratio: 72:88) ΔwetA. These mostly involved component, biological process, molecular function rarely associated with development. Conclusively, plays relatively conserved role spore surface structure, also differentiated some other processes redundant robertsii unlike its ortholog B. bassiana. IMPORTANCE regulate maturation required cycle like they do remain unexplored pathogen considered evolved pathogenicity ~130 million years later This study reveals similar development, pathogenicity, distinctive mediating maturation-related events, has redundancy robertsii. process vital proves dependent assembly independent accumulation. function. Our unveils or different from those learned bassiana ascomycetes.

Language: Английский

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Monomeric streptavidin phage display allows efficient immobilization of bacteriophages on magnetic particles for the capture, separation, and detection of bacteria

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 27, 2023

Abstract Immobilization of bacteriophages onto solid supports such as magnetic particles has demonstrated ultralow detection limits biosensors for the separation and their host bacteria. While potential impact magnetized phages is high, current methods immobilization are either weak, costly, inefficient, or laborious making them less viable commercialization. In order to bridge this gap, we have developed a highly efficient, site-specific, low-cost method immobilize supports. streptavidin–biotin represents an ideal conjugation method, functionalization with streptavidin requires square meters coverage therefore not amenable assay. Here, genetically engineered allow synthesis monomeric during infection bacterial host. The was fused capsid protein (Hoc) site-specific self-assembly up 155 fusion proteins per capsid. Biotin coated nanoparticles were functionalized mSA-Hoc T4 phage in E. coli assay limit < 10 CFU 100 mLs water. This work highlights creation modified novel modification, expanding bacteriophage biotechnologies.

Language: Английский

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Ubr1-mediated ubiquitylation orchestrates asexual development, polar growth, and virulence-related cellular events in Beauveria bassiana

Applied Microbiology and Biotechnology, Journal Year: 2021, Volume and Issue: 105(7), P. 2747 - 2758

Published: March 8, 2021

Language: Английский

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Physiological response of Metarhizium rileyi with linoleic acid supplementation

Fungal Biology, Journal Year: 2024, Volume and Issue: 128(4), P. 1827 - 1835

Published: May 16, 2024

Language: Английский

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