The Bbotf1 Zn(Ⅱ)2Cys6 transcription factor contributes to antioxidant response, fatty acid assimilation, peroxisome proliferation and infection cycles in insect pathogenic fungus Beauveria bassiana

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: 204, P. 108083 - 108083

Published: March 7, 2024

Language: Английский

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Comparative roles of three adhesin genes (adh1–3) in insect-pathogenic lifecycle of Beauveria bassiana

Applied Microbiology and Biotechnology, Journal Year: 2021, Volume and Issue: 105(13), P. 5491 - 5502

Published: June 25, 2021

Language: Английский

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Circadian oscillations in Trichoderma atroviride and the role of core clock components in secondary metabolism, development, and mycoparasitism against the phytopathogen Botrytis cinerea

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Aug. 11, 2022

Circadian clocks are important for an individual’s fitness, and recent studies have underlined their role in the outcome of biological interactions. However, relevance circadian fungal–fungal interactions remains largely unexplored. We sought to characterize a functional clock biocontrol agent Trichoderma atroviride assess its importance mycoparasitic interaction against phytopathogen Botrytis cinerea . Thus, we confirmed existence rhythms T. , which temperature-compensated modulated by environmental cues such as light temperature. Nevertheless, presence molecular appears be highly dependent on nutritional composition media. Complementation null ( Δfrq ) Neurospora crassa strain with -negative component tafrq restored core function, same period observed latter fungus, confirming bona fide component. Confrontation assays between wild-type mutant strains B. constant or darkness, revealed inhibitory effect atroviride’s capabilities. Interestingly, when confrontation were performed under light/dark cycles, atroviride’ s overgrowth capacity was enhanced inoculations at dawn compared dusk. Deleting clock-negative element FRQ but not vital daily differential phenotype, suggesting that has more significant influence result this interaction. Additionally, components modulate development secondary metabolism including rhythmic production distinct volatile organic compounds (VOCs). study provides evidence how impact diverse aspects lifestyle changes fungal dynamics.

Language: Английский

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Circadian clock is critical for fungal pathogenesis by regulating zinc starvation response and secondary metabolism

Science Advances, Journal Year: 2025, Volume and Issue: 11(13)

Published: March 28, 2025

Circadian clocks are known to modulate host immune responses pathogen infections, yet their role in influencing pathogenesis remains unclear. Here, we investigated the of circadian regulating fungal Fusarium oxysporum , which has multiple genes homologous Neurospora crassa frq due gene duplication events, with Fofrq1 being primary clock gene. The F. plants is controlled by its clock, infections causing severe disease symptoms at dawn. Notably, disruption dramatically reduces pathogenicity. regulate rhythmic expression several transcription factors, including FoZafA, enables adapt zinc starvation within plant, and FoCzf1, governs production toxin fusaric acid. Together, our findings highlight critical roles pathogenicity response secondary metabolite production.

Language: Английский

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Hydrophobin CmHYD1 Is Involved in Conidiation, Infection and Primordium Formation, and Regulated by GATA Transcription Factor CmAreA in Edible Fungus, Cordyceps militaris

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(8), P. 674 - 674

Published: Aug. 20, 2021

Hydrophobins are a family of small proteins exclusively secreted by fungi, and play variety roles in the life cycle. Cmhyd1, one hydrophobin class II members Cordyceps militaris, has been shown to have high transcript level during fruiting body development. Here, deletion Cmhyd1 results reduction aerial mycelia, conidiation, hydrophobicity infection ability, complete inhibition pigmentation primordium differentiation. plays conidiation cuticle-bypassing regulating transcripts frequency clock protein, Cmfrq, velvet Cmvosa, as well formation via mitogen-activated protein kinase signaling pathway. also participates stress response, including tolerance mycelia osmotic oxidative stresses, conidia or low temperatures. CmAreA, transcription factor nitrogen regulatory, is recruited promoter activates with coactivator CmOTam using electrophoretic mobility shift assays transient luciferase expression tobacco. Furthermore, CmHYD1 proved regulate Cmarea at different developmental stages positive feedback loop. These reveal diverse regulation C. provide insights into regulatory mechanism mushrooms.

Language: Английский

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Differential Roles of Five Fluffy Genes (flbA–flbE) in the Lifecycle In Vitro and In Vivo of the Insect–Pathogenic Fungus Beauveria bassiana

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(4), P. 334 - 334

Published: March 23, 2022

The fluffy genes flbA-flbE are well-known players in the upstream developmental activation pathway that activates key gene brlA of central (CDP) to initiate conidiation Aspergillus nidulans. Here, we report insignificant roles their orthologs radial growth Beauveria bassiana under normal culture conditions and different stresses although flbA flbD were involved respective responses heat shock H2O2. Aerial level was lowered deletion mutants flbB flbE (~15%) less than flbC (~30%), which CDP abaA repressed consistently during incubation. CDP-controlled blastospore production submerged cultures mimicking insect hemolymph abolished mutant with being sharply repressed, decreased by 55% only downregulated. fungal virulence against a model attenuated absence more irrespective cuticle infection or cuticle-bypassing (intrahemocoel injection). These findings unravel important role flbC, but null flbB/D/E, B. bassiana's insect-pathogenic lifecycle scenario distinctive from A.nidulans.

Language: Английский

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Distinctive role of fluG in the adaptation of Beauveria bassiana to insect‐pathogenic lifecycle and environmental stresses

Environmental Microbiology, Journal Year: 2021, Volume and Issue: 23(9), P. 5184 - 5199

Published: April 5, 2021

Summary The upstream developmental activation (UDA) pathway comprises three fluG ‐cored cascades ( fluG‐flbA , fluG‐flbE/B/D and fluG‐flbC ) that activate the key gene brlA of central (CDP) to initiate conidiation in aspergilli. However, core role remains poorly understood other fungi. Here, we report distinctive insect‐pathogenic lifecycle Beauveria bassiana . Disruption resulted limited defect, which was mitigated with incubation time associated time‐course up‐regulation/down‐regulation all flb CDP genes another ‐like (BBA_06309). In Δ increased sensitivities various stresses correlated repression corresponding stress‐responsive genes. Its virulence through normal cuticle infection attenuated greatly due blocked secretion cuticle‐degrading enzymes delayed formation hyphal bodies (blastospores) accelerate proliferation vivo host death. submerged cultures mimicking insect haemolymph, largely blastospore production concurred drastic up‐regulation abaA earlier most cultures. Our results unveil an essentiality for fungal adaptation suggest act as alternative player UDA B

Language: Английский

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Comprehensive in silico analysis of the underutilized crop tef (Eragrostis tef (Zucc.) Trotter) genome reveals drought tolerance signatures

BMC Plant Biology, Journal Year: 2023, Volume and Issue: 23(1)

Published: Oct. 21, 2023

Abstract Background Tef ( Eragrostis tef ) is a C 4 plant known for its tiny, nutritious, and gluten-free grains. It contains higher levels of protein, vitamins, essential minerals like calcium (Ca), iron (Fe), copper (Cu), zinc (Zn) than common cereals. cultivated in diverse ecological zones under climatic conditions. Studies have shown that has great diversity withstanding environmental challenges such as drought. Drought major abiotic stress severely affecting crop productivity becoming bottleneck to global food security. Here, we used silico -based functional genomic analysis identify drought-responsive genes validated their expression using quantitative RT-PCR. Results We identified about 729 so far reported six plants, including rice, wheat, maize, barley, sorghum, pearl millet, the model Arabidopsis, 20 having high-level GO terms related drought, significantly enriched several biological molecular function categories. These were found play roles, water fluid transport, resistance high salt, cold, drought stress, abscisic acid (ABA) signaling, de novo DNA methylation, transcriptional regulation other crops. Our revealed substantial differences conserved domains some from well-studied rice orthologs. further analyzed sixteen orthologs RT-PCR response PEG-induced osmotic stress. Conclusions The findings showed differential shoots, roots, or both tissues. Hence, this study may be promising candidates trait improvement crops via transgenic gene-editing technologies.

Language: Английский

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DIM5/KMT1 controls fungal insect pathogenicity and genome stability by methylation of histone H3K4, H3K9 and H3K36

Virulence, Journal Year: 2021, Volume and Issue: 12(1), P. 1306 - 1322

Published: May 6, 2021

Mono-, di- and tri-methylation of histone H3 Lys 9, 4, 36 (H3K_me1/me2/me3) required for mediation DNA-based cellular events in eukaryotes usually rely upon the activities lysine methyltransferases (KMTs) classified to KMT1, KMT2, KMT3 families, respectively. Here, an H3K9-specific DIM5/KMT1 orthologue, which lacks a C-terminal post-SET domain localizes mainly nucleus, is reported have both conserved noncanonical roles methylating core lysines Beauveria bassiana, insect-pathogenic fungus serving as main source wide-spectrum fungal insecticides. Disruption dim5 led abolishment H3K9me3 marked attenuation H3K4me1/me2, H3K9me1/me2 H3K36me2. Consequently, Δdim5 mutant lost whole insect pathogenicity through normal cuticle infection, was compromised severely virulence cuticle-bypassing infection (hemocoel injection) also series critical lifecycle vivo vitro, including reduced hyphal growth, blocked conidiation, impeded proliferation vivo, altered carbohydrate epitopes, disturbed cell cycle, biosynthesis secretion cuticle-degrading enzymes, increased sensitivities various stresses. Among 1,201 dysregulated genes (up/down ratio: 712:489) associated with those phenotypic changes, 92 59:33) encode transcription factors proteins or enzymes involved posttranslational modifications, implying that DIM5-methylated could act preferential marks transcription-active crucial global gene regulation. These findings uncover novel scenario DIM5 its indispensability lifestyle genome stability B. bassiana.

Language: Английский

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The Essential and the Nonessential Roles of Four Clock Elements in the Circadian Rhythm of Metarhiziumrobertsii

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(6), P. 558 - 558

Published: May 25, 2022

FRQ (frequency protein), FRH (FRQ-interacting RNA helicase), and WC1 WC2 (white collar proteins) are major clock elements that govern the circadian rhythm in Neurosporacrassa. However, deletion of frh is lethal for viability N. crassa, making it elusive whether essential or nonessential rhythm. This needs clarification a fungus where not lethal. Here, nuclear ortholog proved Metarhiziumrobertsii. The nucleocytoplasmic shuttling M. robertsii FRQ, WC1, orthologs was light-dependent. Yeast two-hybrid assay validated interactions with instead WC2. well, shown as conidiation rings tint dark 15 d-old plate cultures grown at 25 °C light/dark cycle 12:12, abolished absence frq wc1, partially disturbed wc2, but unaffected frh. These results indicate requirement either fungal Further analyses mutants revealed dispensable limited roles insect-pathogenic lifecycle robertsii, respectively.

Language: Английский

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