Subcellular biochemistry and biology of filamentous entomopathogenic fungi

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 35 - 58

Published: Jan. 1, 2024

Language: Английский

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Effect of ultraviolet radiation on Beauveria bassiana virulence and development of protective formulations

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(4)

Published: March 10, 2023

Language: Английский

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Only one of three hydrophobins (Hyd1–3) contributes to conidial hydrophobicity and insect pathogenicity of Metarhizium robertsii

Journal of Invertebrate Pathology, Journal Year: 2023, Volume and Issue: 201, P. 108006 - 108006

Published: Oct. 14, 2023

Language: Английский

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SET1/KMT2‐governed histone H3K4 methylation coordinates the lifecycle in vivo and in vitro of the fungal insect pathogen Beauveria bassiana

Environmental Microbiology, Journal Year: 2021, Volume and Issue: 23(9), P. 5541 - 5554

Published: Aug. 14, 2021

Summary Biological control potential of insect‐pathogenic fungi against pests is an overall output various cellular processes regulated by signalling and epigenetic networks. In Beauveria bassiana , mono/di/trimethylation histone H3 Lys 4 (H3K4me1/me2/m3) was abolished inactivation the lysine methyltransferase SET1/KMT2, leading to marked virulence loss, reductions in conidial hydrophobicity adherence insect cuticle, impeded proliferation vivo severe defects growth conidiation, increased sensitivities cell wall perturbation, H 2 O heat shock. Such compromised phenotypes correlated well with transcriptional abolishment or repression carbon catabolite‐repressing transcription factor Cre1, classes I II hydrophobins Hyd1 Hyd2 required for hydrophobicity, key developmental regulators, stress‐responsive enzymes/proteins. Particularly, expression cre1 which upregulates hyd4 upon activation KMT2‐mediated H3K4me3 Metarhizium robertsii nearly Δ set1 mutant, hyd1 hyd2 as homologues . These data suggest that SET1‐Cre1‐Hyd1/2 pathway function B like KMT2‐Cre1‐Hyd4 elucidated mediate pathogenicity M Our findings unveil not only a regulatory role SET1‐cored fungal but also its novel mediating asexual cycle vitro stress responses

Language: Английский

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FluG and FluG-like FlrA Coregulate Manifold Gene Sets Vital for Fungal Insect-Pathogenic Lifestyle but Not Involved in Asexual Development

mSystems, Journal Year: 2022, Volume and Issue: 7(4)

Published: July 11, 2022

FluG is a core regulator upstream of central developmental pathway (CDP) in Aspergillus nidulans but multiple FluG-like regulators (FLRs) remain functionally uncharacterized ascomycetes. Our previous study revealed no role for the CDP activation and an existence sole FLR (FlrA) insect-pathogenic fungus.

Language: Английский

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Essential Role of WetA, but No Role of VosA, in Asexual Development, Conidial Maturation and Insect Pathogenicity of Metarhizium robertsii

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 14, 2023

Conidial maturation, which is crucial for conidial quality, controlled by the asexual development activator WetA and downstream, velvety protein VosA in Aspergillus. Their orthologs have proved functional quality control of Beauveria bassiana, as seen Aspergillus, but are functionally unexplored, Metarhizium robertsii, another hypocrealean insect pathogen. Here, prove essential nonessential M. robertsii's life cycle, respectively. Disruption wetA increased hyphal sensitivity to oxidative stress Congo red-induced cell wall stress, had little impact on radial growth. The ΔwetA mutant was severely compromised conidiation capacity featured slower germination, decreased UV resistance, reduced hydrophobicity, deformed hydrophobin rodlet bundles that were assembled onto coat. mutant's virulence greatly attenuated via normal infection due a blockage infection-required cellular processes. All examined phenotypes unaffected ΔvosA mutant. Intriguingly, mannitol much less accumulated 7- 15-day-old cultures than strains, while trehalose not detectable at all, revealing link intracellular polyol accumulation maturation. Transcriptomic analysis revealed differential regulation 160 genes (up/down ratio: 72:88) ΔwetA. These mostly involved component, biological process, molecular function rarely associated with development. Conclusively, plays relatively conserved role spore surface structure, also differentiated some other processes redundant robertsii unlike its ortholog B. bassiana. IMPORTANCE regulate maturation required cycle like they do remain unexplored pathogen considered evolved pathogenicity ~130 million years later This study reveals similar development, pathogenicity, distinctive mediating maturation-related events, has redundancy robertsii. process vital proves dependent assembly independent accumulation. function. Our unveils or different from those learned bassiana ascomycetes.

Language: Английский

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Two sirtuin proteins, Hst3 and Hst4, modulate asexual development, stress tolerance, and virulence by affecting global gene expression in Beauveria bassiana

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(2)

Published: Jan. 9, 2024

Beauveria bassiana is a widely used entomopathogenic fungus in insect biological control applications. In this study, we investigated the role of two sirtuin homologs, BbHst3 and BbHst4, activities pathogenicity B. bassiana. Our results showed that deletion and/or BbHst4 led to impaired sporulation, reduced (~50%) conidial production, decreased tolerance various stresses, including osmotic, oxidative, cell wall-disturbing agents. Moreover, plays dominant roles histone H3-K56 acetylation DNA damage response, while more responsible for maintaining wall integrity. Transcriptomic analyses revealed significant changes (>1,500 differentially expressed genes) gene expression patterns mutant strains, particularly genes related secondary metabolism, detoxification, transporters. Furthermore, ΔBbHst3, ΔBbHst4, ΔBbHst3ΔBbHst4 strains exhibited virulence bioassays, with (~20%) abilities kill hosts through topical application intra-hemocoel injection. These findings highlight crucial repair, integrity, fungal infection study provides new insights into regulatory mechanisms underlying emphasizes potential targeting sirtuins improving efficacy biocontrol agents.IMPORTANCESirtuins, as class deacetylases, have been shown play important cellular processes fungi, asexual development, stress pathogenicity. By investigating functions uncovered their critical contributions phenotypes Deletion these homologs yield, increased sensitivity osmotic oxidative repair processes, virulence. differential numerous involved transporters, virulence-related factors, potentially uncovering targets manipulation optimization also significance key regulators biology highlights promising development novel antifungal strategies.

Language: Английский

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Essential Role of COP9 Signalosome Subunit 5 (Csn5) in Insect Pathogenicity and Asexual Development of Beauveria bassiana

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(8), P. 642 - 642

Published: Aug. 7, 2021

Csn5 is a subunit ofthe COP9/signalosome complex in model fungi. Here, we report heavier accumulation of orthologous the nucleus than cytoplasm and its indispensability to insect pathogenicity virulence-related cellular events Beauveria bassiana. Deletion csn5 led 68% increase intracellular ubiquitin dysregulation 18 genes encoding ubiquitin-activating (E1), -conjugating (E2), -ligating (E3) enzymes ubiquitin-specific proteases, suggesting role balanced ubiquitination/deubiquitination. Consequently, deletion mutant displayed abolished pathogenicity, marked reductions conidial hydrophobicity adherence cuticle, secretion cuticle penetration-required enzymes, blocked haemocoel colonisation, reduced conidiation capacity despite unaffected biomass accumulation. These phenotypes correlated well with sharply repressed or expressions key hydrophobin required for biosynthesis/assembly developmental activator essential aerial submerged blastospore production. In mutant, increased sensitivities heat shock oxidative stress also expression levels several heat-responsive decreased activities antioxidant enzymes. Altogether, Csn5-reliant ubiquitination/deubiquitination balance coordinates those crucial quality control functionally important which are collectively fungal events, asexual development.

Language: Английский

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Genome-Wide Insight into Profound Effect of Carbon Catabolite Repressor (Cre1) on the Insect-Pathogenic Lifecycle of Beauveriabassiana

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(11), P. 895 - 895

Published: Oct. 23, 2021

Carbon catabolite repression (CCR) is critical for the preferential utilization of glucose derived from environmental carbon sources and regulated by repressor A (Cre1/CreA) in filamentous fungi. However, a role Cre1-mediated CCR insect-pathogenic fungal host nutrients during normal cuticle infection (NCI) hemocoel colonization remains explored insufficiently. Here, we report an indispensability Cre1 Beauveriabassiana's insect integument hemocoel. Deletion cre1 resulted severe defects radial growth on various media, hypersensitivity to oxidative stress, abolished pathogenicity via NCI or intrahemocoel injection (cuticle-bypassing infection) but no change conidial hydrophobicity adherence cuticle. Markedly reduced biomass accumulation Δcre1 cultures was directly causative defect aerial conidiation secretion cuticle-degrading enzymes. The majority (1117) 1881 dysregulated genes identified versus wild-type were significantly downregulated, leading substantial many enriched function terms pathways, particularly those involved nitrogen metabolisms, degradation, antioxidant response, cellular transport homeostasis, direct/indirect gene mediation. These findings offer novel insight into profound effect lifestyle B. bassiana.

Language: Английский

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Fungal insecticidal activity elevated by non‐risky markerless overexpression of an endogenous cysteine‐free protein gene in Beauveria bassiana

Pest Management Science, Journal Year: 2022, Volume and Issue: 78(7), P. 3164 - 3172

Published: April 26, 2022

Fungal insecticides are notorious for slow kill action, an intrinsic trait that can be improved by the genetic engineering of exogenous or endogenous virulence factor. However, transgenic expressing toxin and herbicide-resistant marker genes may cause unexpected ecological risks hardly permitted field release due to strict regulatory hurdles. It is necessary improve biotechnology speed up fungal insect-killing action exclude risk source.A markerless transformation system Beauveria bassiana, a main source wide-spectrum insecticides, was reconstructed based on uridine auxotrophy (Δura3). The applied overexpression small cysteine-free protein (120 amino acids) gene cfp previously characterized as regulator expression. Three cfp-overexpressed strains showed much faster Galleria mellonella larvae than parental wild-type via normal cuticle infection but no change in vegetative growth aerial condition. achieved not only significant increases conidial adherence insect total activity secreted cuticle-degrading Pr1 proteases antioxidant enzymes crucial collapse immune defense acceleration hemocoel localization, proliferation vivo host death from mummification.The free any foreign DNA fragment ecologic provides novel biotechnological approach enhancing insecticidal with non-risky striding over © 2022 Society Chemical Industry.

Language: Английский

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