AoSte12 Is Required for Mycelial Development, Conidiation, Trap Morphogenesis, and Secondary Metabolism by Regulating Hyphal Fusion in Nematode-Trapping Fungus Arthrobotrys oligospora

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Feb. 14, 2023

Nematode-trapping (NT) fungi are a unique group of carnivorous microorganisms that can capture and digest nematodes by producing ingenious trapping devices (traps).

Language: Английский

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The cAMP‐PKA signalling pathway regulates hyphal growth, conidiation, trap morphogenesis, stress tolerance, and autophagy in Arthrobotrys oligospora

Environmental Microbiology, Journal Year: 2022, Volume and Issue: 24(12), P. 6524 - 6538

Published: Oct. 19, 2022

The cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signalling pathway is evolutionarily conserved in eukaryotes and plays a crucial role defending against external environmental challenges, which can modulate the cellular response to stimuli. Arthrobotrys oligospora typical nematode-trapping fungus that specializes adhesive networks kill nematodes. To elucidate biological roles of cAMP-PKA pathway, we characterized orthologous adenylate cyclase AoAcy, regulatory subunit (AoPkaR), two catalytic subunits (AoPkaC1 AoPkaC2) PKA A. by gene disruption, transcriptome, metabolome analyses. Deletion Aoacy significantly reduced levels cAMP arthrobotrisins. Results revealed Aoacy, AopkaR, AopkaC1 were involved hyphal growth, trap morphogenesis, sporulation, stress resistance, autophagy. In addition, regulation mitochondrial morphology, thereby affecting energy metabolism, whereas AopkaC2 affected nuclei, Multi-omics results showed regulated multiple metabolic processes. Collectively, these data highlight indispensable development, pathogenicity oligospora, provide insights into mechanisms pathways formation, lifestyle transition.

Language: Английский

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The antifungal peptide AnAFP from Aspergillus niger promotes nutrient mobilization through autophagic recycling during asexual development

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 24, 2025

Antifungal peptides are promising drug candidates to fight fungal infections in the clinics and agriculture. However, recent data suggest that antifungal might also play a role within their own producing organism survive nutrient limiting conditions. We have therefore studied function of AnAFP Aspergillus niger more detail. To achieve this, we established Tet-on controlled anafp expression system, which allowed us study null an overexpression phenotype same isolate. observed increased intracellular reduces growth A. prematurely activates autophagy. Comparative transcriptome analyses glucose-starving mycelium demonstrated strongly impacts genes important for cell wall integrity remodeling, as well with predicted metabolism transport carbohydrates, proteins, lipids. Notably, encoding regulators conidiophore development such flbC flbD became induced upon overexpression. Fluorescent driven AnAFP::eGFP fusion protein congruently unraveled localizes walls septa . Moreover, is spatially restricted selected compartments only affected cells displayed sudden reduction hyphal diameter. From these conclude drive vegetative sporulation during limitation through autophagic recycling. predict drives mobilization selective lysis ensure survival whole colony phases starvation.

Language: Английский

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Transcriptomic and proteomic insights into aerial conidiation of Metarhizium rileyi mediated by maltose and glucose

Pesticide Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 106467 - 106467

Published: May 1, 2025

Language: Английский

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Roles of BrlA and AbaA in Mediating Asexual and Insect Pathogenic Lifecycles of Metarhizium robertsii

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(10), P. 1110 - 1110

Published: Oct. 21, 2022

BrlA and AbaA are key activators of the central developmental pathway (CDP) that controls asexual development in Aspergillus but their roles remain insufficiently understood hypocerealean insect pathogens. Here, regulatory orthologs Metarhizium robertsii (Clavicipitaceae) were characterized for comparison to those elucidated previously Beauveria bassiana (Cordycipitaceae) at phenotypic transcriptomic levels. Time-course transcription profiles brlA, abaA, other CDP activator gene wetA revealed they not so sequentially activated M. as learned Aspergillus. Aerial conidiation essential fungal infection dispersal, submerged blastospore production mimicking yeast-like budding proliferation hemocoel, pathogenicity via cuticular penetration all abolished a consequence brlA or abaA disruption, which had little impact on normal hyphal growth. The disruptants severely compromised virulence cuticle-bypassing (intrahemocoel injection) differentially impaired cellular tolerance oxidative cell wall-perturbing stresses. ΔbrlA ΔabaA mutant shad 255 233 dysregulated genes (up/down ratios: 52:203 101:122) respectively, including 108 co-dysregulated. These counts small compared with 1513 2869 707:806 1513:1356) identified mutants B. bassiana. Results only conserved control also indispensable adaptation insect-pathogenic lifecycle host habitats. Intriguingly, BrlA- AbaA-controlled expression networks largely different between two pathogens, similar phenotypes absence either abaA.

Language: Английский

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Recovery of insect-pathogenic fungi from solar UV damage: Molecular mechanisms and prospects

Advances in applied microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 59 - 82

Published: Jan. 1, 2024

Language: Английский

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CRISPR-Cas9-mediated enhancement of Beauveria bassiana virulence with overproduction of oosporein

Fungal Biology and Biotechnology, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 21, 2024

Biocontrol agents play a pivotal role in managing pests and contribute to sustainable agriculture. Recent advancements genetic engineering can facilitate the development of entomopathogenic fungi with desired traits enhance biocontrol efficacy. In this study, CRISPR-Cas9 ribonucleoprotein system was utilized genetically improve virulence Beauveria bassiana, broad-spectrum insect pathogen used arthropod worldwide. CRISPR-Cas9-based disruption transcription factor-encoding gene Bbsmr1 led derepression oosporein biosynthetic cluster resulting overproduction red-pigmented dibenzoquinone involved host immune evasion, thus increasing fungal virulence. Mutants defective for displayed remarkable enhanced insecticidal activity by reducing lethal times concentrations, while concomitantly presenting negligible or minor pleiotropic effects. addition, these mutants faster germination on cuticle which correlated higher density free-floating blastospores hemolymph accelerated mortality host. These findings emphasize utility developing customized phenotypic traits, provide an efficient versatile transformation tool application other beneficial fungi.

Language: Английский

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Essential Role of WetA, but No Role of VosA, in Asexual Development, Conidial Maturation and Insect Pathogenicity of Metarhizium robertsii

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: March 14, 2023

Conidial maturation, which is crucial for conidial quality, controlled by the asexual development activator WetA and downstream, velvety protein VosA in Aspergillus. Their orthologs have proved functional quality control of Beauveria bassiana, as seen Aspergillus, but are functionally unexplored, Metarhizium robertsii, another hypocrealean insect pathogen. Here, prove essential nonessential M. robertsii's life cycle, respectively. Disruption wetA increased hyphal sensitivity to oxidative stress Congo red-induced cell wall stress, had little impact on radial growth. The ΔwetA mutant was severely compromised conidiation capacity featured slower germination, decreased UV resistance, reduced hydrophobicity, deformed hydrophobin rodlet bundles that were assembled onto coat. mutant's virulence greatly attenuated via normal infection due a blockage infection-required cellular processes. All examined phenotypes unaffected ΔvosA mutant. Intriguingly, mannitol much less accumulated 7- 15-day-old cultures than strains, while trehalose not detectable at all, revealing link intracellular polyol accumulation maturation. Transcriptomic analysis revealed differential regulation 160 genes (up/down ratio: 72:88) ΔwetA. These mostly involved component, biological process, molecular function rarely associated with development. Conclusively, plays relatively conserved role spore surface structure, also differentiated some other processes redundant robertsii unlike its ortholog B. bassiana. IMPORTANCE regulate maturation required cycle like they do remain unexplored pathogen considered evolved pathogenicity ~130 million years later This study reveals similar development, pathogenicity, distinctive mediating maturation-related events, has redundancy robertsii. process vital proves dependent assembly independent accumulation. function. Our unveils or different from those learned bassiana ascomycetes.

Language: Английский

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A novel fungal sensor (Ngs1) of N-acetylglucosamine (GlcNAc) mediates the fungal response to GlcNAc in the interaction between entomopathogenic Beauveria bassiana and insect host

Journal of Invertebrate Pathology, Journal Year: 2024, Volume and Issue: unknown, P. 108206 - 108206

Published: Sept. 1, 2024

Language: Английский

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N-acetylglucosamine sensor, Ngs1 contributes to Beauveria bassiana vegetative growth, oxidative phosphorylation, fungal development, and cell wall integrity during entomopathogen-insect interaction

Pesticide Biochemistry and Physiology, Journal Year: 2024, Volume and Issue: 208, P. 106273 - 106273

Published: Dec. 24, 2024

Language: Английский

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