Mechanisms of probiotic Bacillus against enteric bacterial infections

One Health Advances, Journal Year: 2023, Volume and Issue: 1(1)

Published: Aug. 15, 2023

Abstract Gastrointestinal infection is a leading cause of gut diseases attracting global health concerns. The emerging antimicrobial resistance in enteric pathogens drives the search viable and renewable alternatives to antibiotics for both human beings animals. Spore-forming probiotic Bacillus have received extensively interests their multiple benefits, including restoration microbiota dysbiosis reduction drug-resistant pathogens. These promising benefits are mainly attributed activity structurally diverse -derived metabolites, such as antibacterial compounds, short-chain fatty acids, other small molecules. Such metabolites show capacity directly target either individual or community bacterial pathogens, potentiate host cells microbiota. better understanding mechanisms by which modulate metabolism hosts will advance screening development . In this review, we discuss interaction among , host, summarize Bacillus- derived that act key players interactions, shedding light on mechanistic against infections.

Language: Английский

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Optimization of the biosynthesis of silver nanoparticles using bacterial extracts and their antimicrobial potential

Biotechnology Reports, Journal Year: 2023, Volume and Issue: 40, P. e00816 - e00816

Published: Oct. 29, 2023

In the present study, silver nanoparticles (AgNPs) were biosynthesized using supernatant and intracellular extract of Cupriavidus necator, Bacillus megaterium, subtilis. The characterization AgNPs was carried out UV-Vis spectroscopy, FTIR, DLS TEM. Resazurin microtiter-plate assay used to determine antimicrobial action against Escherichia coli. UV-Visible spectra showed peaks between 414 460 nm. TEM analysis revealed that synthesized mostly spherical shapes. results determined sizes from 20.8 118.4 highest activity obtained with rather than those extract. Therefore, it bacterial species, temperature, pH, type (supernatant or intracellular) influence biosynthesis. This synthesis thus offers a simple, environmentally friendly, low-cost method for production AgNPs, which can be as antibacterial agents.

Language: Английский

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Pangenome analyses of Bacillus pumilus, Bacillus safensis, and Priestia megaterium exploring the plant-associated features of bacilli strains isolated from canola

Molecular Genetics and Genomics, Journal Year: 2022, Volume and Issue: 297(4), P. 1063 - 1079

Published: May 25, 2022

Language: Английский

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Antagonistic and growth-promoting potential of multifarious bacterial endophytes against Fusarium wilt of chickpea

Egyptian Journal of Biological Pest Control, Journal Year: 2022, Volume and Issue: 32(1)

Published: Feb. 22, 2022

Abstract Background Chickpea is the most important legume crop in India, and its productivity significantly lowered due to biotic constraints such as wilt caused by Fusarium oxysporum f. sp . ciceris Endophytes help plants thrive better under stress conditions regulating plant hormones inducing systemic resistance. The aim of present study was identify chickpea root bacterial endophytes having antagonistic ability for management wilt. Results Twenty endophytic strains were isolated from susceptible resistant cultivar, amongst which 35% gave positive results siderophore 15% isolates HCN producers, whereas 55% showed good growth on ACC-supplemented medium. Based upon 16S rRNA phylogenetic analysis, efficient CRBE1, CRBE3 CRBE7 identified Priestia megaterium, Brucella haematophila Microbacterium paraoxydans , respectively. Bacterial endophyte pronounced activity against sp. vitro conditions. Inoculation with alone combination through seed priming resulted significant biocontrol screenhouse Biopriming seeds consortium + upsurged phenol content varieties JG 62 HC 3, indicated role promising potential bio-agents vivo Conclusions megaterium (CRBE1), (CRBE3) (CRBE7) exhibiting could be incorporated integrated disease module chickpea.

Language: Английский

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Co-Inoculation of Endophytes Bacillus siamensis TUR07-02b and Priestia megaterium SMBH14-02 Promotes Growth in Rice with Low Doses of Nitrogen Fertilizer

Plants, Journal Year: 2023, Volume and Issue: 12(3), P. 524 - 524

Published: Jan. 23, 2023

Multiple biotic and abiotic factors influence rice cultivation. These limit productivity yield, as well an irrational use of agrochemicals in A sustainable alternative is using selected growth-promoting microorganisms to increase nutritional efficiency. In the present study, direct mechanisms growth promotion two strains Bacillus, three Priestia, Burkholderia endophytes were characterized. Bacillus siamensis TUR07-02b Priestia megaterium SMBH14-02 promote Oryza sativa var's growth. "Bellavista" was used at different doses (50, 75, 100%) mineral nitrogen (N) a randomized block design by quintuplicate. Both strains, TUR07-02b, presented outstanding promoter characteristics, including auxin production (123.17 335.65 μg mL-1, respectively) biological fixation capacity. Similarly, B. could solubilize phosphate-Ca (20.94 mL-1), cellulases, pectinases. Under greenhouse conditions, co-inoculated plants receiving 75% total dose showed increased agronomic parameters relation panicle length, grains per panicle, grain harvest index 25.0, 30.7, 39.5, 12.5%, respectively, compared fertilized treatment without inoculation. The P. are potential microbial resources formulation new inoculants reduce nitrogenous fertilizers. Thus, validation inoculant consortium field level will be essential step providing for management cultivation farmers San Martín region.

Language: Английский

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Microbial degradation of low-density polyethylene, polyethylene terephthalate, and polystyrene by novel isolates from plastic-polluted environment

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(4)

Published: March 22, 2024

Language: Английский

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Antimicrobial activity and comparative metabolomic analysis of Priestia megaterium strains derived from potato and dendrobium

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: March 31, 2023

Abstract The growth of endophytic bacteria is influenced by the host plants and their secondary metabolites activities. In this study, P. megaterium P-NA14 D-HT207 were isolated from potato tuber dendrobium stem respectively. They both identified as Priestia . antimicrobial activities strains explored. For activities, results showed that exhibited a stronger inhibition effect on pathogen dendrobium, while potato. supernatant an only Staphylococcus aureus , sediment Escherichia coli metabolomic analysis, content L -phenylalanine in was higher than D-HT207, several key downstream associated with S. including tyrosine, capsaicin, etc. Therefore, we speculated different between possibly related to its metabolites. This study preliminarily explored why same hosts exhibit perspective metabolomics.

Language: Английский

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Archaeal and Bacterial Response to a Severe Pulse Disturbance: A Shotgun Metagenomic Study

Published: Jan. 1, 2025

Language: Английский

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Anticancer and Antioxidant Activities of Rhizospheric Soil Bacteria of Lophocereus marginatus

International Journal of Microbiology, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Identification of new sources compounds with anticancer and antioxidant potential is essential for cancer treatment. Rhizospheric bacteria have emerged as a promising source these agents. This study aimed at investigating the properties isolated from rhizosphere Lophocereus marginatus. Six rhizobacteria L. marginatus were molecularly identified by sequencing 16S rRNA gene MALDI-TOF. We evaluated inhibitory activity methanol (MeOH) ethyl acetate (EtOAc) extracts obtained against murine lymphoma cell line L5178Y-R, well normal Vero monkey kidney cells peripheral blood mononuclear (PBMCs). Compared those cells, MeOH extract Paenibacillus sp. PMS-B023 showed selective lymphoma, 50% concentration (IC50) values 117.5 μg/mL selectivity index (SI) > 2.1, compared cells. L-Pro-L-Val cycle, gageostatin B, cinnamic acid, parvifloracin, daidzein, genistein, actinomycin D, Y4, surfactin C detected in extract, which may be responsible its antilymphoma activity. did not find significant any using 2,2-diphenyl-1-picrylhydrazyl technique (IC50 250 μg/mL). The results that has antitumor L5178Y-R presence was also demonstrated, previously described literature.

Language: Английский

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The Isolation of Free-Living Nitrogen-Fixing Bacteria and the Assessment of Their Potential to Enhance Plant Growth in Combination with a Commercial Biostimulant

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(3), P. 69 - 69

Published: March 18, 2025

The development of microbial-based biostimulants to enhance the growth crops and support a healthy sustainable soil requires isolation large-scale industrial culture effective microorganisms. In this study, work was undertaken isolate characterize free-living nitrogen-fixing bacteria capable acting as alone or by incorporation into and/or supplementation with current commercial crop biostimulant for farmers. Free-living were isolated from soil, sugar cane mulch, plant roots following preliminary in nitrogen-free media that targeted specific groups known diazotrophs. Following identification each 16S rDNA sequence analysis, isolates selected further study identified most closely related Priestia megaterium, Sphingobium yanoikuyae, Burkholderia paludis. Each investigated its capacity promote media. Wheat seedlings inoculated separately, together consortium, combination biostimulant, Great Land Plus®. Compared no-treatment control plants, fresh weights higher both shoots (183.2 mg vs. 330.6 mg; p < 0.05) (320.4 731.3 wheat P. megaterium. also (267.8 (610.3 = S. yanoikuyae. contrast, weight shoot root systems plants B. paludis significantly lower (p than plants. Moreover, when Plus® supplemented consortium megaterium no promotion observed.

Language: Английский

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