Stress-Tolerant Endophytic Isolate Priestia aryabhattai BPR-9 Modulates Physio-Biochemical Mechanisms in Wheat (Triticum aestivum L.) for Enhanced Salt Tolerance

International Journal of Environmental Research and Public Health, Journal Year: 2022, Volume and Issue: 19(17), P. 10883 - 10883

Published: Sept. 1, 2022

In efforts to improve plant productivity and enhance defense mechanisms against biotic abiotic stresses, endophytic bacteria have been used as an alternative chemical fertilizers pesticides. the current study, 25 microbes recovered from organs of

Language: Английский

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Genomic analysis reveals genes that encode the synthesis of volatile compounds by a Bacillus velezensis-based biofungicide used in the treatment of grapes to control Aspergillus carbonarius

International Journal of Food Microbiology, Journal Year: 2024, Volume and Issue: 415, P. 110644 - 110644

Published: Feb. 27, 2024

Language: Английский

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Prospecting the Functional Potential of Bacillus altitudinis 1.4 Isolated from Sediment in Association with Bradyrhizobium japonicum

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(3)

Published: Feb. 10, 2025

Language: Английский

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Pangenome analysis of Bacillus velezensis exploring the probiotic potential and plant growth promotion traits of strains isolated from fish intestines

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: Feb. 18, 2025

Language: Английский

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Whole-Genome Sequence Insight into the Plant-Growth-Promoting Bacterium Priestia filamentosa Strain AZC66 Obtained from Zygophyllum coccineum Rhizosphere

Plants, Journal Year: 2023, Volume and Issue: 12(10), P. 1944 - 1944

Published: May 10, 2023

This study aimed to isolate, screen the plant-growth-enhancing features, and explore whole-genome sequence of AZC66 isolated from rhizosphere Zygophyllum coccineum determine its biostimulating effects on growth cowpea under greenhouse conditions. Salkowski reagent was used measure AZC66’s indole acetic acid production. inorganic phosphate solubility Pikovskaya agar evaluated using tricalcium phosphate. The results indicated ability fix nitrogen, produce IAA (66.33 ± 0.44 μg mL−1), solubilize phosphate, exhibit activity ACC deaminase (278.40 21 mol -ketobutyrate mg−1 h−1). Cowpea’s root shoot dry weights were also significantly increased after in vitro inoculation with AZC66. identity confirmed as Priestia filamentosa, 4840 genes predicted genome. gene sequences compared against Kyoto Encyclopedia Genes Genomes (KEGG) database, showed that top three pathways wherein maximum number are involved signaling cellular processes, genetic information processing, carbohydrate metabolism. genome sequencing strain revealed a implicated plant biostimulation activities such nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), solubilization (PhbCEF, pstABCS, phoU), siderophore formation (FbpA, feoAB, fetB). contained numerous metabolism, regulation, nitrate reduction pathway. phenazine biosynthetic demonstrated biocontrol soil survival properties. trehalose may help plants resist osmotic salt stress. discovery glycine betaine, cold shock, heat shock protein could withstand harsh might be create robust, sustainable biological fertilizers for future agricultural use Saudi Arabia. Furthermore, adaptable metabolic serve basis potential biotechnological applications agriculture industry.

Language: Английский

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The Complex GH32 Enzyme Orchestra from Priestia megaterium Holds the Key to Better Discriminate Sucrose-6-phosphate Hydrolases from Other β-Fructofuranosidases in Bacteria

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(2), P. 1302 - 1320

Published: Jan. 4, 2024

Inulin is widely used as a prebiotic and emerging priming compound to counteract plant diseases. We isolated inulin-degrading strains from the lettuce phyllosphere, identified Bacillus subtilis Priestia megaterium, species hosting well-known biocontrol organisms. To better understand their varying inulin degradation strategies, three intracellular β-fructofuranosidases P. megaterium NBRC15308 were characterized after expression in Escherichia coli: predicted sucrose-6-phosphate (Suc6P) hydrolase (SacAP1, supported by molecular docking), an exofructanase (SacAP2), invertase (SacAP3). Based on protein multiple sequence structure alignments of bacterial glycoside family 32 enzymes, we conserved residues be involved binding phosphorylated (Suc6P hydrolases) or nonphosphorylated substrates (invertases fructanases). Suc6P hydrolases feature positively charged near structural catalytic pocket (histidine, arginine, lysine), whereas other contain tryptophans. This correlates with our phylogenetic tree, grouping all clan associated genomic regions coding for transporters substrate phosphorylation. These results will help discriminate between future studies interaction B. endophytes sucrose and/or fructans, sugars naturally present plants exogenously applied context defense priming.

Language: Английский

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Significance of zinc-solubilizing plant growth-promoting rhizobacterial strains in nutrient acquisition, enhancement of growth, yield, and oil content of canola (Brassica napus L.)

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 27, 2024

The present study was conducted with the aim to isolate, characterize, and identify promising zinc-solubilizing rhizobacteria found naturally in rhizosphere of canola ( Brassica napus L.) plants. investigated roles these strains nutrient acquisition assimilation extracellular molecules such as hormones secondary metabolites. Ten isolated (CLS1, CLS2, CLS3, CLS6, CLS8, CLS9, CLS11, CLS12, CLS13, CLS15) were selected characterized biochemically. Almost all tested Gram-positive, could fix nitrogen, positive for indole acetic acid, HCN, exopolysaccharides, siderophore production. These effective identified through 16S rRNA gene sequencing. Based on amount solubilized zinc halo zone diameter, four potent CLS9) pot field evaluation. Among bacterial genera from same host plant at different sampling sites, Priestia aryabhattai most abundant three sites. megaterium , Staphylococcus succinus Bacillus cereus two subtilis only one site. have a number growth-stimulating characteristics well ability colonize roots successfully. results indicated that inoculation enhanced growth, oil contents, yield attributes compared non-inoculated control fertilizer levels. (CLS1) first reported solubilizer associated canola. (CLS2) (CLS9) be best strains, pronounced beneficial effect growth traits both conditions. site-specific dominance observed this may contribute toward decision-making development specific inocula Therefore, identification help providing adequate soluble along yield, content

Language: Английский

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Sporulation, Structure Assembly, and Germination in the Soil Bacterium Bacillus thuringiensis: Survival and Success in the Environment and the Insect Host

Microbiology Research, Journal Year: 2023, Volume and Issue: 14(2), P. 466 - 491

Published: April 4, 2023

Bacillus thuringiensis (Bt) is a rod-shaped, Gram-positive soil bacterium that belongs to the phylum Firmicutes and genus Bacillus. It spore-forming bacterium. During sporulation, it produces wide range of crystalline proteins are toxic different orders insects. Sporulation, structure assembly, germination essential stages in cell cycle B. thuringiensis. The majority studies on these issues have focused model organism subtilis, followed by cereus anthracis. machinery for sporulation extrapolated However, light recent findings concerning role (SPoVS), receptors (Gr), cortical enzymes Bt, theory strengthened conservation programs drive survival success environment insect host. In present minireview, latter pinpointed reviewed.

Language: Английский

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Plant growth-promoting and heavy metal-resistant Priestia and Bacillus strains associated with pioneer plants from mine tailings

Archives of Microbiology, Journal Year: 2023, Volume and Issue: 205(9)

Published: Aug. 24, 2023

Language: Английский

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Pan-genomic comparison of a potential solvent-tolerant alkaline protease-producing Exiguobacterium sp. TBG-PICH-001 isolated from a marine habitat

3 Biotech, Journal Year: 2023, Volume and Issue: 13(11)

Published: Oct. 16, 2023

Language: Английский

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