BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: April 1, 2025
Chicory
is
a
unique
and
nutritious
vegetable
crop.
However,
the
molecular
mechanisms
underlying
anthocyanin
biosynthesis
in
chicory
remain
poorly
understood.
We
combined
transcriptomics
metabolomics
analyses
to
explore
basis
of
red-budded
(Z1)
yellow-budded
(Z7)
chicory.
Integrated
were
performed
investigate
A
total
26
key
structural
genes,
including
F3'H,
DFR,
CHS,
ANS,
identified
enriched
pathways
such
as
flavonoid
biosynthesis.
Additionally,
29
transcription
factors
identified,
11
MYB,
five
bHLH,
two
WD40
factors,
with
seven
MYB
genes
upregulated
four
downregulated,
indicating
their
roles
regulating
Notably,
factor,
CI35997,
which
homologous
RLL2A
lettuce,
was
predicted
positively
regulate
Other
AP2/ERF,
bZIP,
NAC,
Trihelix,
have
also
been
implicated.
Metabolomics
analysis
revealed
that
cyanidin
derivatives
main
contributors
red
coloration
buds,
cyanidin-3-O-(6-O-malonyl)-glucoside
being
most
abundant.
Furthermore,
competitive
relationship
between
lignin
observed,
wherein
downregulation
lignin-related
enhanced
accumulation.
This
study
offer
molecular-level
insights
into
These
findings
provide
valuable
guidance
for
genetic
improvement
other
crops
high
content.
ABSTRACT
Phytochemical
accumulation
plays
a
vital
role
in
plant
defense.
This
study
examines
the
effect
of
high‐pressure
(HP)
treatment
on
phenolic
biosynthesis
and
gene
expression
harvested
strawberries,
evaluating
HP
as
potential
enhancer
through
environmental
stress
responses.
Strawberries
were
subjected
to
at
pressures
ranging
from
10
40
MPa
two
or
three
cycles.
led
significant
increases
total
content,
up
1.59
times
higher
than
untreated
controls.
Gene
analysis
showed
upregulation
key
biosynthetic
genes—phenylalanine
ammonia‐lyase,
chalcone
synthase,
UDP‐glucose
flavonoid
3‐
O
‐glucosyl
transferase—with
peak
observed
after
cycles
MPa.
effectively
enhances
compound
synthesis
by
activating
pathways.
method
presents
practical,
non‐genetic
strategy
for
improving
nutritional
profiles
fruits.
Molecules,
Journal Year:
2025,
Volume and Issue:
30(5), P. 1184 - 1184
Published: March 6, 2025
Flavonoids
represent
a
class
of
natural
plant
secondary
metabolites
with
multiple
activities
including
antioxidant,
antitumor,
anti-inflammatory,
and
antimicrobial
properties.
However,
due
to
their
structural
characteristics,
they
often
exhibit
low
bioavailability
in
vivo.
In
this
review,
we
focus
on
the
vivo
study
flavonoids,
particularly
effects
gut
microbiome
common
modifications
such
as
methylation,
acetylation,
dehydroxylation,
etc.
These
aim
change
characteristics
original
substances
enhance
absorption
bioavailability.
order
improve
discuss
two
feasible
methods,
namely
dosage
form
modification
chemical
modification,
hope
that
these
approaches
will
offer
new
insights
into
application
flavonoids
for
human
health.
article,
also
introduce
types,
sources,
efficacy
flavonoids.
conclusion,
is
comprehensive
review
how
Physiologia Plantarum,
Journal Year:
2025,
Volume and Issue:
177(2)
Published: March 1, 2025
Anthocyanin
accumulation
plays
a
crucial
role
in
enhancing
Lilium
petal
colouration;
however,
breeding
efforts
are
hindered
by
our
lack
of
understanding
the
complex
molecular
mechanism
behind
pigment's
synthesis.
This
study
explores
potential
WRKY
family
gene
WRKY75
anthocyanin
synthesis
lilies.
Contrary
to
inhibitory
effect
observed
Arabidopsis
thaliana,
both
transient
silencing
and
overexpression
analyses
LvWRKY75
indicate
that
positively
regulates
The
was
found
cause
significant
upregulation
structural
genes
pivotal
for
biosynthesis
lilies,
including
Lv3GT,
LvDFR
LvANS,
as
well
regulatory
LvMYB5.
Further
in-depth
analyses,
yeast
one-hybrid,
electrophoretic
mobility
shift
assay,
dual-luciferase
assays,
demonstrated
binds
promoter
LvMYB5,
its
transcriptional
activity.
In
turn,
increased
expression
LvMYB5
upregulates
transcription
downstream
such
LvANS.
summary,
this
provides
deeper
mechanisms
contributing
improving
strategies
flowers'
ornamental
value
commercial
appeal.
BMC Plant Biology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: April 1, 2025
Chicory
is
a
unique
and
nutritious
vegetable
crop.
However,
the
molecular
mechanisms
underlying
anthocyanin
biosynthesis
in
chicory
remain
poorly
understood.
We
combined
transcriptomics
metabolomics
analyses
to
explore
basis
of
red-budded
(Z1)
yellow-budded
(Z7)
chicory.
Integrated
were
performed
investigate
A
total
26
key
structural
genes,
including
F3'H,
DFR,
CHS,
ANS,
identified
enriched
pathways
such
as
flavonoid
biosynthesis.
Additionally,
29
transcription
factors
identified,
11
MYB,
five
bHLH,
two
WD40
factors,
with
seven
MYB
genes
upregulated
four
downregulated,
indicating
their
roles
regulating
Notably,
factor,
CI35997,
which
homologous
RLL2A
lettuce,
was
predicted
positively
regulate
Other
AP2/ERF,
bZIP,
NAC,
Trihelix,
have
also
been
implicated.
Metabolomics
analysis
revealed
that
cyanidin
derivatives
main
contributors
red
coloration
buds,
cyanidin-3-O-(6-O-malonyl)-glucoside
being
most
abundant.
Furthermore,
competitive
relationship
between
lignin
observed,
wherein
downregulation
lignin-related
enhanced
accumulation.
This
study
offer
molecular-level
insights
into
These
findings
provide
valuable
guidance
for
genetic
improvement
other
crops
high
content.
