Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Jan. 17, 2024
Abstract
Cardiovascular
diseases
(CVDs)
encompass
various
conditions
affecting
the
heart
and
its
blood
vessels
are
often
linked
with
oral
microbes.
Our
data
analysis
aimed
to
identify
bacteria
from
other
non-oral
sites
(i.e.,
gut,
arterial
plaque
cultured
blood)
that
could
be
CVDs.
Taxonomic
profiling
identified
species
level
compared
Human
Oral
Microbiome
Database
(HOMD).
The
in
samples
were
catalogued,
their
average
frequency
calculated
for
each
sample.
Additionally,
filtered
by
comparison
Project
(HMP)
database.
We
17,243
microbial
species,
of
which
410
present
HOMD
database
further
denominated
as
“oral”,
found
at
least
one
gut
sample,
but
only
221
169
samples,
respectively.
Of
153
solely
oral-associated
environments
after
HMP
database,
irrespective
presence
body
sites.
results
suggest
a
potential
connection
between
specific
bacterial
occurrence
Detecting
these
patients
CVDs
help
uncover
link
health
general
health,
including
cardiovascular
via
translocation.
Environmental Microbiome,
Journal Year:
2024,
Volume and Issue:
19(1)
Published: May 6, 2024
Abstract
Background
Hot
spring
biofilms
provide
a
window
into
the
survival
strategies
of
microbial
communities
in
extreme
environments
and
offer
potential
for
biotechnological
applications.
This
study
focused
on
green
brown
thriving
submerged
plant
litter
within
Sungai
Klah
hot
Malaysia,
characterised
by
temperatures
58–74
°C.
Using
Illumina
shotgun
metagenomics
Nanopore
ligation
sequencing,
we
investigated
diversity
functional
metagenome-assembled
genomes
(MAGs)
with
specific
focus
biofilm
formation,
heat
stress
response,
carbohydrate
catabolism.
Results
Leveraging
power
both
short-reads
long-reads,
employed
an
Illumina-Nanopore
hybrid
assembly
approach
to
construct
MAGs
enhanced
quality.
The
dereplication
process,
facilitated
dRep
tool,
validated
efficiency
assembly,
yielding
that
reflected
intricate
these
ecosystems.
comprehensive
analysis
uncovered
intriguing
insights
thermophilic
taxa
biofilms.
Moreover,
examined
degradation
biofilms,
shedding
light
participation
diverse
breakdown
starch,
cellulose,
hemicellulose.
We
highlight
Chloroflexota
Armatimonadota
exhibited
wide
array
glycosyl
hydrolases
targeting
various
substrates,
underscoring
their
metabolic
versatility
utilisation
carbohydrates
at
elevated
temperatures.
Conclusions
advances
understanding
ecology
under
temperature
revealing
adaptation
from
In
addition,
our
findings
biotechnology
application
through
identification
lignocellulose-degrading
enzymes.
By
demonstrating
utilising
reads,
value
combining
multiple
sequencing
methods
more
thorough
exploration
complex
communities.
Applied Microbiology and Biotechnology,
Journal Year:
2024,
Volume and Issue:
108(1)
Published: May 10, 2024
Abstract
Xylanases
are
key
biocatalysts
in
the
degradation
of
β‐1,4‐glycosidic
linkages
xylan
backbone
hemicellulose.
These
enzymes
potentially
applied
a
wide
range
bioprocessing
industries
under
harsh
conditions.
Metagenomics
has
emerged
as
powerful
tools
for
bioprospection
and
discovery
interesting
bioactive
molecules
from
extreme
ecosystems
with
unique
features,
such
high
temperatures.
In
this
study,
an
innovative
combination
function-driven
screening
compost
metagenomic
library
automatic
extraction
halo
areas
in-house
MATLAB
functions
resulted
identification
promising
clone
xylanase
activity
(LP4).
The
LP4
proved
to
be
effective
producer
submerged
fermentation
Sequence
phylogenetic
analyses
revealed
that
xylanase,
Xyl4,
corresponded
endo-1,4-β-xylanase
belonging
glycosyl
hydrolase
family
10
(GH10).
When
xyl4
was
expressed
Escherichia
coli
BL21(DE3),
enzyme
increased
about
2-fold
compared
clone.
To
get
insight
on
interaction
substrate
establish
possible
strategies
improve
its
activity,
structure
Xyl4
predicted,
refined,
docked
xylohexaose.
Our
data
unveiled,
first
time,
relevance
amino
acids
Glu133
Glu238
catalysis,
close
inspection
catalytic
site
suggested
replacement
Phe316
by
bulkier
Trp
may
activity.
current
findings
contribute
enhancing
performance
towards
industrial
applications.
Key
points
•
A
GH10
(Xyl4)
isolated
MATLAB’s
were
developed
identify
xylanase-producing
clones
Computational
analysis
showed
crucial
residues
catalysis
Graphical
abstract
