Filamentous fungi as cell factories for heterogeneous protein production DOI

Pallavi Meshram,

Meenakshi Murmu,

Sagar Barage

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 143 - 169

Published: Sept. 13, 2024

Language: Английский

Exploring the potential of Aspergillus wentii: secondary metabolites and biological properties DOI
Bayan E. Ainousah, Sabrin R. M. Ibrahim, Abdulrahim A. Alzain

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(5)

Published: April 15, 2024

Language: Английский

Citations

2
From lab to table: The path of recombinant milk proteins in transforming dairy production. DOI
Igor Negreiros Piazenski, João Pedro Manica Candelário, Vanete Thomaz‐Soccol

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 149, P. 104562 - 104562

Published: May 24, 2024

Language: Английский

Citations

2
Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products DOI Creative Commons
Xinran Xu, Yanhong Sun,

Anxin Zhang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract Microbial genetic circuits are vital for regulating gene expression and synthesizing bioactive compounds. However, assessing their strength timing, especially in multicellular fungi, remains challenging. Here, an advanced microfluidic platform is combined with a mathematical model enabling precise characterization of fungal regulatory (GRCs) at the single‐cell level. Utilizing this platform, intensity timing 30 transcription factor‐promoter combinations derived from two representative GRCs, using fungus Aspergillus nidulans determined. As proof concept, selected GRC combination utilized to successfully refactor biosynthetic pathways molecules, precisely control production, activate silenced clusters (BGCs). This study provides insights into microbial regulation highlights potential synthetic biology applications discovery novel natural products.

Language: Английский

Citations

2
An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system DOI Creative Commons
Yijian Wu,

Sai Feng,

Zeao Sun

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2023, Volume and Issue: 11

Published: Oct. 5, 2023

Saccharomyces cerevisiae is one of the most extensively used biosynthetic systems for production diverse bioproducts, especially biotherapeutics and recombinant proteins. Because expression insertion foreign genes are always impaired by endogenous factors nonproductive procedures, various technologies have been developed to enhance strength efficiency transcription facilitate gene editing procedures. Thus, limitations that block heterologous protein secretion overcome. Highly efficient promoters responsible initiation accurate regulation can be precisely regulated with synthetic double promoter systems. Appropriate codon optimization harmonization adaption genomic abundance S. expected further improve translation efficiency. Efficient translocation achieved fusing a specifically designed signal peptide an upstream newly synthesized In addition widely applied engineering technology clear mechanism endoplasmic reticulum secretory pathway, innovative genome technique CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated system) its derivative tools allow more precise disruption, site-directed mutation, insertion. This review focuses on sophisticated techniques emerging genetic metabolic system.

Language: Английский

Citations

6
Filamentous fungi as cell factories for heterogeneous protein production DOI

Pallavi Meshram,

Meenakshi Murmu,

Sagar Barage

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 143 - 169

Published: Sept. 13, 2024

Language: Английский

Citations

1