Epigallocatechin gallate protects mice from Salmonella enterica ser. Typhimurium infection by modulating bacterial virulence through quorum sensing inhibition DOI Creative Commons
Guoqiang Cheng,

Shanqiu Jian,

Wen Li

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Oct. 16, 2024

Salmonella enterica ser. Typhimurium is a common pathogen that poses considerable public health threat, contributing to severe gastrointestinal diseases and widespread foodborne illnesses. The virulence of S. regulated by quorum sensing (QS) the type III secretion system (T3SS). This study investigated inhibitory effects anti-QS activity epigallocatechin gallate (EGCG), which bioactive ingredient found in green tea, on Typhimurium. In vitro bacterial experiments demonstrated EGCG inhibited production autoinducers, biofilm formation, flagellar downregulating expression AI-1, AI-2, pathogenicity islands (SPI)-1, SPI-2, genes related flagella, fimbriae, curli fibers. mouse model Typhimurium-induced enteritis, considerably reduced intestinal colonization S . alleviated damage. conclusion, protects intestines mice infected with inhibiting QS-induced gene expression, demonstrating its potential as therapeutic agent for controlling infections.

Language: Английский

Critical review on plant-derived quorum sensing signaling inhibitors in pseudomonas aeruginosa DOI
Kayeen Vadakkan, Kuppusamy Sathishkumar,

Viji Ouseph Mapranathukaran

et al.

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 151, P. 107649 - 107649

Published: July 15, 2024

Language: Английский

Citations

4
Targeting quorum sensing in Pseudomonas aeruginosa with high-affinity inhibitors: A high-throughput screening and in-silico analysis DOI
Sidra Abbas, Shama Mujawar, Azra Yasmin

et al.

Computational Biology and Chemistry, Journal Year: 2025, Volume and Issue: 117, P. 108419 - 108419

Published: March 11, 2025

Language: Английский

Citations

0
Phytochemical Composition and Biological Properties of Macleania rupestris Fruit Extract: Insights into Its Antimicrobial and Antioxidant Activity DOI Creative Commons
Arianna Mayorga-Ramos, Johana Zúñiga-Miranda, Elena Coyago-Cruz

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 394 - 394

Published: March 27, 2025

Macleania rupestris, a fruit-bearing species of the Ericaceae family, has traditionally been used for its potential medicinal properties. Background/Objectives: This study investigates phytochemical composition and antimicrobial activity M. rupestris fruit extract, focusing on antibacterial, antibiofilm, antifungal effects. Methods: (Kunth) A.C.Sm. berries (code: 4456, Herbario QUPS-Ecuador) were collected from cloud forest Montano Alto, Cuenca-Ecuador, extract was obtained using an ethanolic-based extraction chemically characterized. The antibacterial assessed against seven multidrug-resistant bacteria strains four fungal microdilution method. biofilm inhibition evaluated microplate assay with crystal violet staining antioxidant DPPH ABTS assays. Results: bioactive compounds showed 853.9 mg phenols/100 g DW, 573.2 organic acid/100 21.5 C-3-gl/100 DW anthocyanins. assays demonstrated significant inhibitory Enterococcus faecalis, faecium, Escherichia coli, Staphylococcus epidermidis, MIC values ranging 1.25 to 5 mg/mL. Additionally, confirmed disrupt bacterial biofilms, particularly in S. aureus L. monocytogenes. Nevertheless, no observed Candida spp., suggesting selective Finally, strong (1.62 mmol TE/100 by 3.28 ABTS). Conclusions: These findings indicate that possesses promising properties, which may be attributed phenolic acid composition. Further fractionation targeted bioassays are required elucidate specific responsible these effects explore their applications formulations.

Language: Английский

Citations

0
ChangQing Compound Relieves Eimeria tenella Infection Symptoms by Modulating Intestinal Probiotic and Pathogenic Bacteria Balance DOI
Jiajia Yang, Xuan Wang, Ying Chen

et al.

The Veterinary Journal, Journal Year: 2025, Volume and Issue: unknown, P. 106343 - 106343

Published: April 1, 2025

Language: Английский

Citations

0
Dihydromyricetin alleviates ETEC K88-induced intestinal inflammatory injury by inhibiting quorum sensing-related virulence factors DOI Creative Commons
Yaqian Shi,

Jin Liu,

Hang Zhou

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 9, 2025

Language: Английский

Citations

0
Mechanisms of intestinal pharmacokinetic natural product-drug interactions DOI

Victoria O. Oyanna,

John D. Clarke

Drug Metabolism Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 17

Published: July 30, 2024

The growing co-consumption of botanical natural products with conventional medications has intensified the need to understand potential effects on drug safety and efficacy. This review delves into intricacies intestinal pharmacokinetic interactions between drugs, such as alterations in solubility, permeability, transporter activity, enzyme-mediated metabolism. It emphasizes importance understanding how dissolution, osmolality interplay constituents gastrointestinal tract, potentially altering absorption systemic exposure. Unlike reviews that focus primarily enzyme mechanisms, this article highlights lesser known but equally important mechanisms interaction. Applying Biopharmaceutics Drug Disposition Classification System (BDDCS) can serve a framework for predicting these interactions. Through comprehensive examination specific byakkokaninjinto, green tea catechins, goldenseal, spinach extract, quercetin, we illustrate diversity their dependence physicochemical properties involved. is vital healthcare professionals effectively anticipate manage product-drug interactions, ensuring optimal patient therapeutic outcomes. By exploring emerging aim broaden scope interaction research encourage studies better elucidate complex mechanisms.

Language: Английский

Citations

3
Multidrug resistance in Pseudomonas aeruginosa: genetic control mechanisms and therapeutic advances DOI Creative Commons

Yuanjing Zhao,

Haoran Xu,

Hui Wang

et al.

Molecular Biomedicine, Journal Year: 2024, Volume and Issue: 5(1)

Published: Nov. 27, 2024

Abstract Pseudomonas aeruginosa is a significant opportunistic pathogen, and its complex mechanisms of antibiotic resistance pose challenge to modern medicine. This literature review explores the advancements made from 1979 2024 in understanding regulatory networks genes , with particular focus on molecular underpinnings these mechanisms. The highlights four main pathways involved drug resistance: reducing outer membrane permeability, enhancing active efflux systems, producing antibiotic-inactivating enzymes, forming biofilms. These are intricately regulated by combination genetic regulation, transcriptional regulators, two-component signal transduction, DNA methylation, small RNA molecules. Through an in-depth analysis synthesis existing literature, we identify key elements mexT ampR argR as potential targets for novel antimicrobial strategies. A profound core control nodes offers new perspective therapeutic intervention, suggesting that modulating could potentially reverse restore bacterial susceptibility antibiotics. looks forward future research directions, proposing use gene editing systems biology further understand develop effective strategies against . expected provide innovative solutions problem infectious diseases.

Language: Английский

Citations

2
A Systematic Hierarchical Virtual Screening Model for RhlR Inhibitors Based on PCA, Pharmacophore, Docking, and Molecular Dynamics DOI Open Access
Jiarui Du, Jiahao Li,

Juqi Wen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 8000 - 8000

Published: July 22, 2024

RhlR plays a key role in the quorum sensing of

Language: Английский

Citations

1
Mutation of Pseudomonas aeruginosa lasI/rhlI diminishes its cytotoxicity, oxidative stress, inflammation, and apoptosis on THP-1 macrophages DOI Creative Commons

Yanying Ren,

Xiaojuan You, Rui Zhu

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(10)

Published: Aug. 20, 2024

ABSTRACT The management of Pseudomonas aeruginosa ( P. ) infections presents a substantial challenge to clinics and public health, emphasizing the urgent need for innovative strategies address this issue. Quorum sensing (QS) is an intercellular communication mechanism that coordinates bacterial activities involved in various virulence mechanisms, such as acquiring host nutrients, facilitating biofilm formation, enhancing motility, secreting factors, evading immune responses, all which play crucial role colonization infection . LasI/R RhlI/R sub-systems dominate QS system Macrophages pivotal host’s innate response invasion, particularly through phagocytosis initial defense mechanism. This study investigated effects ’s on THP-1 macrophages. Mutants PAO1 with lasI/rhlI deletion, well their corresponding complemented strains, were obtained, significant downregulation QS-related genes was observed mutants. Furthermore, ΔlasI ΔlasIΔrhlI mutants exhibited significantly attenuated terms extracellular polymeric substances synthesis, adhesion, factors production. When infected mutants, macrophages enhanced scavenging ability against demonstrated resistance cytotoxicity, oxidative stress, inflammatory response, apoptosis induced by culture supernatants these mutant strains. These findings offer novel insights into mechanisms underlying how mutation attenuates inflammation, IMPORTANCE classified one ESKAPE pathogens poses global health concern. versatile pathogen contributes broad spectrum virulence, thereby constraining therapeutic options serious infections. illustrated plays prominent attenuating affecting factors’ Notably, strains increased phagocytic activity eliminating intracellular bacteria apoptosis. suggest targeted intervention toward anticipated diminish pathogenicity

Language: Английский

Citations

1
Erythromycin disrupts Acinetobacter baumannii biofilms through destruction of the quorum sensing system DOI Creative Commons
Dong Hang, Jianguo Sun, Yiling Liu

et al.

Medicine, Journal Year: 2024, Volume and Issue: 103(36), P. e38341 - e38341

Published: Sept. 6, 2024

Background: This study was conducted to explore the effects of erythromycin on biofilms comprising Acinetobacter baumannii (A ). Methods: To clarify effect A , we collected pure Ab strains isolated and identified from a variety sample types extracted patients in microbiological laboratory our hospital April August 2023, divided them into an experimental group (treated with erythromycin) control (without erythromycin). The morphology quantity biofilm were observed at 24h, 48h, 72h, 5d post-treatment, respectively, expression quorum sensing (QS) system gene (abaI, abaR) mRNA detected by fluorescence quantitative PCR. Results: results showed that baumanniis are prone form multiple drug-resistant (MDR) bacteria, against which most commonly used clinical antibiotics ineffective. Overall, found number bacteria biofilm, formed gradually increased over time, statistical difference ( P < .05). After addition erythromycin, significant improvements formation achieved, indicating can destroy biofilms, inhibiting bacterial growth certain extent. levels abaI abaR role QS became more apparent time. Biofilm is related . treatment, downregulated group. Conclusion: Erythromycin disrupts destroying system.

Language: Английский

Citations

1