Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi

Biotechnology Advances, Journal Year: 2025, Volume and Issue: 81, P. 108561 - 108561

Published: March 12, 2025

Language: Английский

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A Comprehensive Review of the Diversity of Fungal Secondary Metabolites and Their Emerging Applications in Healthcare and Environment

Mycobiology, Journal Year: 2024, Volume and Issue: 52(6), P. 335 - 387

Published: Nov. 1, 2024

Fungi and their natural products, like secondary metabolites, have gained a huge demand in the last decade due to increasing applications healthcare, environmental cleanup, biotechnology-based industries. The fungi produce these metabolites (SMs) during different phases of growth, which are categorized into terpenoids, alkaloids, polyketides, non-ribosomal peptides. These SMs exhibit significant biological activity, contributes formulation novel pharmaceuticals, biopesticides, bioremediation agents. Nowadays, fungal-derived widely used food beverages, for fermentation, preservatives, protein sources, dairy In it is being as an antimicrobial, anticancer, anti-inflammatory, immunosuppressive drug. usage modern tools biotechnology can achieve increase large-scale production. present review comprehensively analyses diversity fungal along with emerging agriculture, sustainability, nutraceuticals. Here, authors reviewed recent advancements genetic engineering, metabolic pathway manipulation, synthetic biology improve production yield SMs. Advancement fermentation techniques, bioprocessing, co-cultivation approaches Investigators further highlighted importance omics technologies understanding regulation biosynthesis SMs, offers drug discovery sustainable agriculture. Finally, addressed potential manipulation biotechnological innovations exploitation commercial benefits.

Language: Английский

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CRISPR/CasRx: A novel antiviral approach to combat largemouth bass (Micropterus salmoides) Rhabdovirus infections

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742189 - 742189

Published: Jan. 1, 2025

Language: Английский

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Expression of a Colletotrichum polyketide synthase gene in Aspergillus nidulans leads to unexpected conjugates with a host metabolite

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 6, 2025

Abstract Heterologous expression of the putative 1,3,6,8-tetrahydroxynaphthalene synthase gene ChPKS from Colletotrichum higginsianum in Aspergillus nidulans led to formation at least eight new compounds. LC-MS analysis proved them as coupling products with an intermediate cichorine biosynthetic pathway. Comprehensive NMR confirmed structures two predominant higginidulans A and B. Deletion backbone pathway host strain abolished higginidulans. alternative Penicillium crustosum resulted expected product 1,3,6,8-tetrahydroxynaphthalene, which was by acetylation structural elucidation. This study provides additional example unexpected natural crosstalk pathways derived different species. Moreover, it highlights importance using systems for expression.

Language: Английский

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Cupin-domain containing protein is not essential for the alkyl salicylaldehyde formation in Aspergillus ustus

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Previous studies demonstrated the requirement of four enzymes including a cupin-domain containing protein for formation alkyl salicylaldehydes and derivatives. Heterologous expression three biosynthetic genes from

Language: Английский

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Biosynthesis of Tetramate Derivatives in Penicillium crustosum Reveals the Involvement of ortho-Quinone Methide in Crosstalk of Multiple Pathways

Organic Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Genome mining and gene deletion experiments in

Language: Английский

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